MT3_ARATH
ID MT3_ARATH Reviewed; 69 AA.
AC O22433;
DT 14-OCT-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Metallothionein-like protein 3;
DE Short=MT-3;
GN Name=MT3 {ECO:0000312|EMBL:AAB67234.1};
GN OrderedLocusNames=At3g15353 {ECO:0000312|Araport:AT3G15353};
GN ORFNames=K7L4.21 {ECO:0000312|EMBL:BAB02170.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Columbia;
RA Bundithya W., Goldsbrough P.B.;
RT "Arabidopsis thaliana metallothionein-like protein (MT3) gene.";
RL Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP PROTEIN SEQUENCE OF 1-8; 42-45 AND 52-69.
RX PubMed=9112777; DOI=10.1104/pp.113.4.1293;
RA Murphy A., Zhou J., Goldsbrough P.B., Taiz L.;
RT "Purification and immunological identification of metallothioneins 1 and 2
RT from Arabidopsis thaliana.";
RL Plant Physiol. 113:1293-1301(1997).
RN [8]
RP TISSUE SPECIFICITY.
RX DOI=10.1046/j.1469-8137.2003.00813.x;
RA Guo W.J., Bundithya W., Goldsbrough P.B.;
RT "Characterization of the Arabidopsis metallothionein gene family: tissue-
RT specific expression and induction during senescence and in response to
RT copper.";
RL New Phytol. 159:369-381(2003).
RN [9]
RP FUNCTION.
RX PubMed=18287486; DOI=10.1104/pp.108.115782;
RA Guo W.J., Meetam M., Goldsbrough P.B.;
RT "Examining the specific contributions of individual Arabidopsis
RT metallothioneins to copper distribution and metal tolerance.";
RL Plant Physiol. 146:1697-1706(2008).
RN [10]
RP FUNCTION.
RX PubMed=24635746; DOI=10.1111/nph.12718;
RA Benatti M.R., Yookongkaew N., Meetam M., Guo W.J., Punyasuk N.,
RA Abuqamar S., Goldsbrough P.;
RT "Metallothionein deficiency impacts copper accumulation and redistribution
RT in leaves and seeds of Arabidopsis.";
RL New Phytol. 202:940-951(2014).
CC -!- FUNCTION: Metallothioneins have a high content of cysteine residues
CC that bind various heavy metals (Probable). Functions as metal chelator
CC of copper (Cu) and zinc (Zn) (PubMed:18287486). Plays a role in Cu
CC homeostasis, specifically in the remobilization of Cu from senescing
CC leaves. The mobilization of Cu from internal sources is important for
CC seed development (PubMed:24635746). {ECO:0000269|PubMed:18287486,
CC ECO:0000269|PubMed:24635746, ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences. {ECO:0000305};
CC Name=1;
CC IsoId=O22433-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Expressed in leaf mesophyll cells, root tips, and
CC at low levels in anthers. {ECO:0000269|Ref.8}.
CC -!- SIMILARITY: Belongs to the metallothionein superfamily. Type 15 family.
CC {ECO:0000305}.
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DR EMBL; AF013959; AAB67234.1; -; Genomic_DNA.
DR EMBL; AP000413; BAB02170.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE75655.1; -; Genomic_DNA.
DR EMBL; AY074340; AAL67036.1; -; mRNA.
DR EMBL; AY091386; AAM14325.1; -; mRNA.
DR EMBL; AK229545; BAF01398.1; -; mRNA.
DR EMBL; AY087213; AAM64769.1; -; mRNA.
DR RefSeq; NP_566509.1; NM_112401.2. [O22433-1]
DR AlphaFoldDB; O22433; -.
DR STRING; 3702.AT3G15353.1; -.
DR PaxDb; O22433; -.
DR PRIDE; O22433; -.
DR ProteomicsDB; 250899; -. [O22433-1]
DR EnsemblPlants; AT3G15353.1; AT3G15353.1; AT3G15353. [O22433-1]
DR GeneID; 820771; -.
DR Gramene; AT3G15353.1; AT3G15353.1; AT3G15353. [O22433-1]
DR KEGG; ath:AT3G15353; -.
DR Araport; AT3G15353; -.
DR TAIR; locus:505006351; AT3G15353.
DR eggNOG; ENOG502S7B3; Eukaryota.
DR HOGENOM; CLU_204176_0_0_1; -.
DR InParanoid; O22433; -.
DR OMA; DGNCKCG; -.
DR PhylomeDB; O22433; -.
DR PRO; PR:O22433; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; O22433; baseline and differential.
DR Genevisible; O22433; AT.
DR GO; GO:0005507; F:copper ion binding; IDA:TAIR.
DR GO; GO:0008270; F:zinc ion binding; IDA:UniProtKB.
DR GO; GO:0006878; P:cellular copper ion homeostasis; ISS:TAIR.
DR InterPro; IPR044671; MT3.
DR PANTHER; PTHR33357; PTHR33357; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Direct protein sequencing; Metal-binding;
KW Metal-thiolate cluster; Reference proteome.
FT CHAIN 1..69
FT /note="Metallothionein-like protein 3"
FT /id="PRO_0000434001"
SQ SEQUENCE 69 AA; 7373 MW; 703B9843078AF3EE CRC64;
MSSNCGSCDC ADKTQCVKKG TSYTFDIVET QESYKEAMIM DVGAEENNAN CKCKCGSSCS
CVNCTCCPN
