MT5_MYXVL
ID MT5_MYXVL Reviewed; 483 AA.
AC Q83730;
DT 10-AUG-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 29-SEP-2021, entry version 97.
DE RecName: Full=Ankyrin repeat domain-containing protein M-T5;
DE AltName: Full=M-T5 protein;
DE Short=M5;
GN Name=m005R; Synonyms=m005L;
OS Myxoma virus (strain Lausanne) (MYXV).
OC Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC Chitovirales; Poxviridae; Chordopoxvirinae; Leporipoxvirus.
OX NCBI_TaxID=31530;
OH NCBI_TaxID=9986; Oryctolagus cuniculus (Rabbit).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8676463; DOI=10.1128/jvi.70.7.4394-4410.1996;
RA Mossman K., Lee S.F., Barry M., Boshkov L., McFadden G.;
RT "Disruption of M-T5, a novel myxoma virus gene member of poxvirus host
RT range superfamily, results in dramatic attenuation of myxomatosis in
RT infected European rabbits.";
RL J. Virol. 70:4394-4410(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Lausanne;
RX PubMed=10562494; DOI=10.1006/viro.1999.0001;
RA Cameron C., Hota-Mitchell S., Chen L., Barrett J.W., Cao J.-X.,
RA Macaulay C., Willer D.O., Evans D.H., McFadden G.;
RT "The complete DNA sequence of myxoma virus.";
RL Virology 264:298-318(1999).
RN [3]
RP FUNCTION.
RX PubMed=16051867; DOI=10.1128/jvi.79.16.10750-10763.2005;
RA Johnston J.B., Wang G., Barrett J.W., Nazarian S.H., Colwill K., Moran M.,
RA McFadden G.;
RT "Myxoma virus M-T5 protects infected cells from the stress of cell cycle
RT arrest through its interaction with host cell cullin-1.";
RL J. Virol. 79:10750-10763(2005).
RN [4]
RP INTERACTION WITH HOST SKP1 AND AKT1.
RX PubMed=19776120; DOI=10.1128/jvi.00963-09;
RA Werden S.J., Lanchbury J., Shattuck D., Neff C., Dufford M., McFadden G.;
RT "The myxoma virus m-t5 ankyrin repeat host range protein is a novel adaptor
RT that coordinately links the cellular signaling pathways mediated by Akt and
RT Skp1 in virus-infected cells.";
RL J. Virol. 83:12068-12083(2009).
CC -!- FUNCTION: Substrate-specific adapter of SKP1-containing E3 ubiquitin-
CC protein ligases which mediate the ubiquitination and subsequent
CC proteasomal degradation of host target proteins including CDKN1B.
CC Disappearance of host CDKN1B correlates with cell cycle progression
CC through the G0/G1 checkpoint. Therefore, viruses in infected cells are
CC protected from diverse innate host antiviral responses normally
CC triggered by G0/G1 cell cycle arrest. {ECO:0000269|PubMed:16051867}.
CC -!- SUBUNIT: Interacts (via PRANC/F-box-like domain) with the SKP1
CC component of the host SCF ubiquitin ligase complex. Interacts (via N-
CC terminus) with host AKT1. {ECO:0000269|PubMed:19776120}.
CC -!- INTERACTION:
CC Q83730; P31749: AKT1; Xeno; NbExp=3; IntAct=EBI-6859930, EBI-296087;
CC Q83730; Q13616: CUL1; Xeno; NbExp=5; IntAct=EBI-6859930, EBI-359390;
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DR EMBL; U43201; AAC55050.1; -; Genomic_DNA.
DR EMBL; AF170726; AAF14892.1; -; Genomic_DNA.
DR EMBL; AF170726; AAF15051.1; -; Genomic_DNA.
DR RefSeq; NP_051718.1; NC_001132.2.
DR RefSeq; NP_051874.1; NC_001132.2.
DR SMR; Q83730; -.
DR DIP; DIP-61134N; -.
DR IntAct; Q83730; 2.
DR GeneID; 932059; -.
DR GeneID; 932077; -.
DR KEGG; vg:932059; -.
DR KEGG; vg:932077; -.
DR Proteomes; UP000000867; Genome.
DR GO; GO:0039646; P:modulation by virus of host G0/G1 transition checkpoint; IEA:UniProtKB-KW.
DR GO; GO:0039648; P:modulation by virus of host protein ubiquitination; IEA:UniProtKB-KW.
DR GO; GO:0039649; P:modulation by virus of host ubiquitin-protein ligase activity; IEA:UniProtKB-KW.
DR GO; GO:0019050; P:suppression by virus of host apoptotic process; IMP:CACAO.
DR Gene3D; 1.25.40.20; -; 2.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR InterPro; IPR018272; PRANC_domain.
DR Pfam; PF09372; PRANC; 1.
DR SMART; SM00248; ANK; 7.
DR SUPFAM; SSF48403; SSF48403; 1.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
PE 1: Evidence at protein level;
KW ANK repeat; G0/G1 host cell cycle checkpoint dysregulation by virus;
KW Host-virus interaction; Modulation of host cell cycle by virus;
KW Modulation of host E3 ubiquitin ligases by virus;
KW Modulation of host ubiquitin pathway by virus; Reference proteome; Repeat;
KW Ubl conjugation pathway.
FT CHAIN 1..483
FT /note="Ankyrin repeat domain-containing protein M-T5"
FT /id="PRO_0000396527"
FT REPEAT 32..63
FT /note="ANK 1"
FT REPEAT 67..101
FT /note="ANK 2"
FT REPEAT 105..137
FT /note="ANK 3"
FT REPEAT 177..210
FT /note="ANK 4"
FT REPEAT 250..279
FT /note="ANK 5"
FT REPEAT 283..312
FT /note="ANK 6"
FT REGION 390..478
FT /note="PRANC/F-box-like"
SQ SEQUENCE 483 AA; 55541 MW; 769E3B7F7E52F586 CRC64;
MDLYGYVSCT PRIRHDVLDG LLNVYDPDEL CSRDTPFRLY LTRYDCTPEG LRLFLTRGAD
VNGVRGSRTS PLCTVLSNKD LGNEAEALAK QLIDAGADVN AMAPDGRYPL LCLLENDRIN
TARFVRYMID RGTSVYVRGT DGYGPVQTYI HSKNVVLDTL RELVRAGATV HDPDKTYGFN
VLQCYMIAHV RSSNVQILRF LLRHGVDSSR GLHATVMFNT LERKISHGVF NRKVLDFIFT
QISINEQNSL DFTPINYCVI HNDRRTFDYL LERGADPNVV NFLGNSCLDL AVLNGNKYMV
HRLLRKTITP DAYTRALNVV NSNIYSIKSY GMSEFVKRHG TLYKALIRSF VKDSDREIFT
YVHIYDYFRE FVDECIRERD AMKADVLDAV SVFDTAFGLV ARPRWKHVRI LSKYVRGVYG
DRVKKILRSL HKRRFKTDRL VRRIADLCGP DGLWTRLPVE VRYSVVDYLT DDEIHDLFVK
IHA
