ID   MTA1_NOSS1              Reviewed;         482 AA.
AC   P0A461; P70802;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 86.
DE   RecName: Full=Type II methyltransferase M.AvaI {ECO:0000303|PubMed:12654995};
DE            Short=M.AvaI {ECO:0000303|PubMed:12654995};
DE            EC=2.1.1.113;
DE   AltName: Full=Modification methylase AvaI;
DE   AltName: Full=N(4) cytosine-specific methyltransferase AvaI;
GN   Name=avaIM; OrderedLocusNames=all3632;
OS   Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576).
OC   Bacteria; Cyanobacteria; Nostocales; Nostocaceae; Nostoc.
OX   NCBI_TaxID=103690;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PCC 7120 / SAG 25.82 / UTEX 2576;
RX   PubMed=11759840; DOI=10.1093/dnares/8.5.205;
RA   Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A.,
RA   Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M.,
RA   Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M.,
RA   Takazawa M., Yamada M., Yasuda M., Tabata S.;
RT   "Complete genomic sequence of the filamentous nitrogen-fixing
RT   cyanobacterium Anabaena sp. strain PCC 7120.";
RL   DNA Res. 8:205-213(2001).
RN   [2]
RP   NOMENCLATURE, AND SUBTYPE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: An alpha subtype methylase that recognizes the double-
CC       stranded sequence 5'-CYCGRG-3', methylates C-1 on both strands, and
CC       protects the DNA from cleavage by the AvaI endonuclease.
CC       {ECO:0000250|UniProtKB:P0A462, ECO:0000303|PubMed:12654995}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = an N(4)-
CC         methyl-2'-deoxycytidine in DNA + H(+) + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:16857, Rhea:RHEA-COMP:11369, Rhea:RHEA-COMP:13674,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:85452, ChEBI:CHEBI:137933; EC=2.1.1.113;
CC   -!- SIMILARITY: Belongs to the N(4)/N(6)-methyltransferase family. N(4)
CC       subfamily. {ECO:0000305}.
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DR   EMBL; BA000019; BAB75331.1; -; Genomic_DNA.
DR   PIR; AI2259; AI2259.
DR   RefSeq; WP_010997777.1; NZ_RSCN01000070.1.
DR   AlphaFoldDB; P0A461; -.
DR   SMR; P0A461; -.
DR   STRING; 103690.17132765; -.
DR   REBASE; 3287; M.AvaI.
DR   PRIDE; P0A461; -.
DR   DNASU; 1107230; -.
DR   EnsemblBacteria; BAB75331; BAB75331; BAB75331.
DR   KEGG; ana:all3632; -.
DR   eggNOG; COG0863; Bacteria.
DR   OMA; WDKRAFR; -.
DR   OrthoDB; 1071106at2; -.
DR   PRO; PR:P0A461; -.
DR   Proteomes; UP000002483; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0015667; F:site-specific DNA-methyltransferase (cytosine-N4-specific) activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.150; -; 2.
DR   InterPro; IPR017985; MeTrfase_CN4_CS.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   SUPFAM; SSF53335; SSF53335; 2.
DR   PROSITE; PS00093; N4_MTASE; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Methyltransferase; Reference proteome; Restriction system;
KW   S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..482
FT                   /note="Type II methyltransferase M.AvaI"
FT                   /id="PRO_0000087918"
SQ   SEQUENCE   482 AA;  56579 MW;  795ED0B445EA9E0C CRC64;
     MTSFELESPI EIKTDPTDLD QESDSFVQEI SRFNKALEQR FRDKMRLHES LSRKIVSFQA
     NKSKPQYRWF KYKEAFSVDL VNQLIFEYEK KSFERILDPF AGAGTMLFAC SDAGIQADGI
     EVLPIGQEII EVRKIIQRQF RREDFLRLIE WYKQKPWNQH NNRKYLNRLR ITDGAYPPET
     EASIERFLFS IEKENILVKQ VLRFALLCIL ESISYTRKDG QYLRWDKRAF RKSGSDKFDK
     GKILDFDEAI TEQIKLILND SFDLISNTLF CYGTQRSGIN LFNASCLKIL PEFEQDFYDC
     IITSPPYCNR YDYTRTYALE LALLGVGERD IVQLRQDMLS CTVENKEKSL IHNWQEALRI
     LDKQELLQSI LRFLERELER KKLNNNGIPR MIKGYFYEMA CVIIECFRVL KNGSPLFMVN
     DNVRYAGIDI SVDLILSNIA EEIGFNVEKI LVLPTGKGNS SQQMGTHGRK TLRKCVYVWR
     KP