MTAA_DICDI
ID MTAA_DICDI Reviewed; 2013 AA.
AC Q1ZXQ8; Q9U5Y1;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 02-MAY-2006, sequence version 1.
DT 25-MAY-2022, entry version 96.
DE RecName: Full=Centrosomal protein 224;
DE Short=CP224;
GN Name=mtaA; ORFNames=DDB_G0268616;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL
RP STAGE, AND DISRUPTION PHENOTYPE.
RC STRAIN=AX2;
RX PubMed=10769206; DOI=10.1242/jcs.113.10.1747;
RA Graef R., Daunderer C., Schliwa M.;
RT "Dictyostelium DdCP224 is a microtubule-associated protein and a permanent
RT centrosomal resident involved in centrosome duplication.";
RL J. Cell Sci. 113:1747-1758(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP FUNCTION, AND SUBUNIT.
RX PubMed=12952061; DOI=10.1023/a:1024450922609;
RA Hestermann A., Rehberg M., Graef R.;
RT "Centrosomal microtubule plus end tracking proteins and their role in
RT Dictyostelium cell dynamics.";
RL J. Muscle Res. Cell Motil. 23:621-630(2002).
RN [4]
RP SUBUNIT, AND SUBCELLULAR LOCATION.
RX PubMed=12134070; DOI=10.1091/mbc.e02-01-0054;
RA Rehberg M., Graef R.;
RT "Dictyostelium EB1 is a genuine centrosomal component required for proper
RT spindle formation.";
RL Mol. Biol. Cell 13:2301-2310(2002).
RN [5]
RP FUNCTION.
RX PubMed=14517319; DOI=10.1091/mbc.e03-04-0242;
RA Graef R., Euteneuer U., Ho T.H., Rehberg M.;
RT "Regulated expression of the centrosomal protein DdCP224 affects
RT microtubule dynamics and reveals mechanisms for the control of
RT supernumerary centrosome number.";
RL Mol. Biol. Cell 14:4067-4074(2003).
RN [6]
RP FUNCTION.
RX PubMed=15186508; DOI=10.1186/1471-2121-5-24;
RA Hestermann A., Graef R.;
RT "The XMAP215-family protein DdCP224 is required for cortical interactions
RT of microtubules.";
RL BMC Cell Biol. 5:24-24(2004).
RN [7]
RP SUBUNIT.
RX PubMed=15800059; DOI=10.1091/mbc.e05-01-0069;
RA Rehberg M., Kleylein-Sohn J., Faix J., Ho T.-H., Schulz I., Graef R.;
RT "Dictyostelium LIS1 is a centrosomal protein required for microtubule/cell
RT cortex interactions, nucleus/centrosome linkage, and actin dynamics.";
RL Mol. Biol. Cell 16:2759-2771(2005).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=AX2;
RX PubMed=16782229; DOI=10.1016/j.ejcb.2006.05.008;
RA Koch K.V., Reinders Y., Ho T.-H., Sickmann A., Graef R.;
RT "Identification and isolation of Dictyostelium microtubule-associated
RT protein interactors by tandem affinity purification.";
RL Eur. J. Cell Biol. 85:1079-1090(2006).
CC -!- FUNCTION: Involved in regulation of microtubule dynamics. Regulates the
CC interaction of microtubules tips with the centrosome and cell cortex.
CC {ECO:0000269|PubMed:10769206, ECO:0000269|PubMed:12952061,
CC ECO:0000269|PubMed:14517319, ECO:0000269|PubMed:15186508}.
CC -!- SUBUNIT: Interacts with eb1 at the microtubule tip, centrosome and
CC kinetochore. Interacts with lis1 in the cortical attachment of
CC microtubules. {ECO:0000269|PubMed:12134070,
CC ECO:0000269|PubMed:12952061, ECO:0000269|PubMed:15800059}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC center, centrosome. Cytoplasm, cytoskeleton. Chromosome, centromere,
CC kinetochore. Note=Associated with the microtubule network. Accumulates
CC at the plus end of microtubules.
CC -!- DEVELOPMENTAL STAGE: Expressed throughout the cell cycle (at protein
CC level). {ECO:0000269|PubMed:10769206}.
CC -!- DISRUPTION PHENOTYPE: Mutants exhibit a disappearance of supernumerary
CC centrosomes and multinuclear cells, and increased sensitivity to the
CC microtubule depolymerizing drug nocodazole. Mutants overexpressing mtaA
CC exhibit supernumerary centrosomes and a cytokinesis defect. Mutants
CC underexpressing mtaA display inhibited growth and a reduction in the
CC number and length of astral microtubes. {ECO:0000269|PubMed:10769206}.
CC -!- SIMILARITY: Belongs to the TOG/XMAP215 family. {ECO:0000305}.
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DR EMBL; AJ012088; CAB56504.1; -; mRNA.
DR EMBL; AAFI02000004; EAS66944.1; -; Genomic_DNA.
DR RefSeq; XP_001134481.1; XM_001134481.1.
DR AlphaFoldDB; Q1ZXQ8; -.
DR STRING; 44689.DDB0232951; -.
DR PaxDb; Q1ZXQ8; -.
DR EnsemblProtists; EAS66944; EAS66944; DDB_G0268616.
DR GeneID; 8616510; -.
DR KEGG; ddi:DDB_G0268616; -.
DR dictyBase; DDB_G0268616; cepJ.
DR eggNOG; KOG1820; Eukaryota.
DR HOGENOM; CLU_000539_2_1_1; -.
DR InParanoid; Q1ZXQ8; -.
DR OMA; EMFRWIG; -.
DR PhylomeDB; Q1ZXQ8; -.
DR PRO; PR:Q1ZXQ8; -.
DR Proteomes; UP000002195; Chromosome 1.
DR GO; GO:0005938; C:cell cortex; IDA:dictyBase.
DR GO; GO:0005813; C:centrosome; IDA:dictyBase.
DR GO; GO:0030139; C:endocytic vesicle; IDA:dictyBase.
DR GO; GO:0000776; C:kinetochore; IDA:dictyBase.
DR GO; GO:0005874; C:microtubule; IDA:dictyBase.
DR GO; GO:0000922; C:spindle pole; IDA:dictyBase.
DR GO; GO:0070840; F:dynein complex binding; IDA:dictyBase.
DR GO; GO:0008017; F:microtubule binding; IDA:dictyBase.
DR GO; GO:0061863; F:microtubule plus end polymerase; IBA:GO_Central.
DR GO; GO:0051010; F:microtubule plus-end binding; IEA:InterPro.
DR GO; GO:0051298; P:centrosome duplication; IMP:dictyBase.
DR GO; GO:0030951; P:establishment or maintenance of microtubule cytoskeleton polarity; IBA:GO_Central.
DR GO; GO:0007030; P:Golgi organization; IMP:dictyBase.
DR GO; GO:0000226; P:microtubule cytoskeleton organization; IMP:dictyBase.
DR GO; GO:0046785; P:microtubule polymerization; IMP:dictyBase.
DR GO; GO:0000281; P:mitotic cytokinesis; IMP:dictyBase.
DR GO; GO:0007052; P:mitotic spindle organization; IBA:GO_Central.
DR Gene3D; 1.25.10.10; -; 5.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR024395; CLASP_N_dom.
DR InterPro; IPR021133; HEAT_type_2.
DR InterPro; IPR034085; TOG.
DR InterPro; IPR045110; XMAP215.
DR PANTHER; PTHR12609; PTHR12609; 1.
DR Pfam; PF12348; CLASP_N; 1.
DR SMART; SM01349; TOG; 5.
DR SUPFAM; SSF48371; SSF48371; 2.
DR PROSITE; PS50077; HEAT_REPEAT; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Centromere; Chromosome; Cytoplasm; Cytoskeleton;
KW Kinetochore; Microtubule; Mitosis; Reference proteome; Repeat.
FT CHAIN 1..2013
FT /note="Centrosomal protein 224"
FT /id="PRO_0000328618"
FT REPEAT 115..153
FT /note="HEAT 1"
FT REPEAT 158..196
FT /note="HEAT 2"
FT REPEAT 200..238
FT /note="HEAT 3"
FT REPEAT 348..386
FT /note="HEAT 4"
FT REPEAT 427..465
FT /note="HEAT 5"
FT REPEAT 724..762
FT /note="HEAT 6"
FT REPEAT 816..854
FT /note="HEAT 7"
FT REPEAT 857..895
FT /note="HEAT 8"
FT REPEAT 899..937
FT /note="HEAT 9"
FT REPEAT 977..1015
FT /note="HEAT 10"
FT REPEAT 1240..1279
FT /note="HEAT 11"
FT REPEAT 1281..1314
FT /note="HEAT 12"
FT REPEAT 1317..1353
FT /note="HEAT 13"
FT REGION 512..557
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1043..1109
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1372..1413
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1695..1809
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1905..1949
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1966..1995
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 517..540
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1695..1800
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 1391
FT /note="N -> NN (in Ref. 1; CAB56504)"
FT /evidence="ECO:0000305"
FT CONFLICT 1929
FT /note="N -> NN (in Ref. 1; CAB56504)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 2013 AA; 223812 MW; D0F9FD82D89EC2C4 CRC64;
MGDEEAPSGS IEDRINHKNW KWRVSGLEEL TTKFRNSIEG SGPLFNEWGP QFKKILADIN
PMSQERALEP LSAFIDRCDC VNKFAASYVG VLVEKLFAST RPRAKEKTIE CLLLTIEADS
AEPVVEALLK GTSSTSPKIL LASLAALTQA LKTFGPKQIP VKLILKQFSP WFENRDKGIR
DQASELFIEI YRWIGKALIP LISEALTPIQ LKALQDQFEK LPTDPAVPLK YTRSEAAKAL
ANASKGIQAK PEVVEEIDPY SLMTAVNILP KLTSEFYEGL QAKKWQERSE QMDKLVTILT
NTPKIETADF SELCKALKKI LADVNVMIVQ KAVVSIGLLA DSLRGGFTSY VKPFITPILE
KFKEKKTSVL QSVHTTMDSL VGKSISLSDI IDELTATMQS KVPQIKQEVL VFICNSITNT
KKPADITKVT KQLTKIFMEA LNDTDSNIRD NASKAFAALG GIIGERAMTP YLNQIDPIKA
KKIKDNMPAV ATPVTITPQP LAPVDLKDID LPVSSSNKKP AAATGNSKSS STTTPTGRSS
NSSPLPPPPS SSDDIKNKLI GAGIVNNEII EGLGKTQWKD RLQAVDDILE NVKGLTADSI
NGMSESIIQL LCDKPSLKES NFQVLSSIFS IFIQCCKNDS NFTQQRCANS YLTTCIEKLT
DVKLKEISSE LLFSTGESIT PHAVFTSIYQ FTSNHKNPKI IADSLVWIQQ AIDEFGIGCC
SNGIQQLKPL LDYTKQCLES TNPDVKKSAI KLLCTIKINI GATLTDFLGD VKKPTMEVLD
REFQKIRDQK PPVPNRQWKG MPPPGSAPVQ IEFPRVDISV KLTPAIITNL SDANWKTRSD
ALDEIERIII DANRKIQPKL GGLIPALKNR LTDNNQKCTI TTLNIIGMLS QAMGGQSFEK
HARLLIPGIL LLLGDSKKPV RDAVISCMNV IVQSDLGFDV FIGSLAAPMI QESAFTRKES
LAWTIVNVTN MKAAPIPSEI NTLAKGIISC LQDKSAEIRS LADNLLSILC TQIPLIEFKK
ELKHVKPASQ PSIQTILDKY YQKTGQPIPP PSKTKQSTSS SSSSSSTTSQ QSSTPSSPQP
IRQQQQQQQQ QPTQPQQQQQ QQQRRSILQS NNGASNCEFI IFDINGKMNR QKTNQIPSWH
FIEPTEEVVE ILQDQVLQCF TEEFANLMFS SLPSNSQHMS DLMIGMIEQN PEAIISVLDI
LFRWITFKLF DTGLASQKRV LKILEILLNK LIDSEYSIGE YEASCLVPIL LEKSGSATNE
QIKQIFKQSI QQLEELCLPN VLFRFAIEMV TSQNWRTRVE VLNVMASIID KNGASVCGNL
KVVIPLITQN LNDSQSKQSS LLCLNKLYSH IKDECFKYSN ISQQDKILII GNNNNNNNNN
NNNNNNNNNN NVQQQQQQQQ QQQQQQPRKS LSTDEMSTQL IGCLELLKNY SITKENIEHT
VEALKQFSGL MANGKLDDVF VNFAEEYFLV LTSILADTFP QVSKDATILR LCKYLIHTII
SILSNKVVAK QCNVRCLEIV LNETIKLYSL AESNSSKQGT ESELSKAFNQ ILLRILQNCN
STILFSTLLQ MMSRTDNDQS IQHPGKYNDL LLRCLLRATK SLTTPSILEE LNVETVLSEI
NSFLKSNPSL DEITRKTTKT LTSELYQNRT NQVVKFTKDI ISKGQQQKYQ YLLNLLIELV
PKQFEDLING NGNGNRISSG GSNNNNNNGG RVSSGGNNNN NSGRENINNI NINSSLDSPI
NDHHNSNNTT TTTTRSRTST GSSNSQQQQL PTTSANTTTT TTTTTTTGKT QSTLSTLKIN
KEPRDYSGKT DSQKKELLIE IFKKIGNKDL TLDGIHDLYF FIREYPDYDI TPNLNSSSQQ
FQAYITRNLK KIKDSMDAPK DKDDEVVNYQ ERLKVIQNTF YNQHNQPSSQ VVNNNNNNNN
NNNNNNNNNI NNNNNNNNNS GGNENIAPQL SNTASMASNT LQRLRTLNPD QNSGSNNNNS
HQNSPSTSSS NDLNSTVASL RQRLAQLTSK SNE
