ID   MTAL1_KLULA             Reviewed;         261 AA.
AC   Q08398; Q6CV29;
DT   01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 96.
DE   RecName: Full=Mating-type protein ALPHA1;
DE   AltName: Full=MATalpha1 protein;
DE   AltName: Full=Transcription activator Alpha1p;
GN   Name=MATALPHA1; Synonyms=alpha-1, alpha1;
GN   and
GN   Name=HMLAPLHA1; OrderedLocusNames=KLLA0C00352g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=8339934; DOI=10.1101/gad.7.8.1584;
RA   Yuan Y.-L.O., Stroke I., Fields S.;
RT   "Coupling of cell identity to signal response in yeast: interaction between
RT   the alpha-1 and STE12 proteins.";
RL   Genes Dev. 7:1584-1597(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=10978277; DOI=10.1093/genetics/156.1.81;
RA   Aastroem S.U., Kegel A., Sjoestrand J.O.O., Rine J.;
RT   "Kluyveromyces lactis Sir2p regulates cation sensitivity and maintains a
RT   specialized chromatin structure at the cryptic alpha-locus.";
RL   Genetics 156:81-91(2000).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Mating type proteins are sequence specific DNA-binding
CC       proteins that act as master switches in yeast differentiation by
CC       controlling gene expression in a cell type-specific fashion.
CC       Transcriptional activator that induces the transcription of alpha-
CC       specific genes (By similarity). Required for mating as an alpha-cell.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00655}.
CC   -!- MISCELLANEOUS: There are three genetic loci for mating type genes in
CC       K.lactis. MAT is the expression locus that determines the mating type
CC       of the cell, whereas HML (containing HMLALPHA1, HMLALPHA2 and
CC       HMLALPHA3) and HMR (containing HMRA1 and HMRA2) represent silenced
CC       repositories of mating type information. The mating type is determined
CC       by the MAT locus, which contains either a copy of HML or of HMR.
CC       Diploid cells are usually heterozygous for the MAT locus.
CC   -!- SIMILARITY: Belongs to the MATALPHA1 family. {ECO:0000255|PROSITE-
CC       ProRule:PRU00655}.
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DR   EMBL; L21155; AAA35252.1; -; Genomic_DNA.
DR   EMBL; AF195066; AAG21092.1; -; Genomic_DNA.
DR   EMBL; CR382123; CAH01060.1; -; Genomic_DNA.
DR   PIR; B47650; B47650.
DR   RefSeq; XP_452210.1; XM_452210.1.
DR   AlphaFoldDB; Q08398; -.
DR   DIP; DIP-329N; -.
DR   STRING; 28985.XP_452210.1; -.
DR   EnsemblFungi; CAH01060; CAH01060; KLLA0_C00352g.
DR   GeneID; 2892594; -.
DR   KEGG; kla:KLLA0_C00352g; -.
DR   eggNOG; ENOG502S4ZK; Eukaryota.
DR   HOGENOM; CLU_1115917_0_0_1; -.
DR   InParanoid; Q08398; -.
DR   OMA; DSECMAK; -.
DR   Proteomes; UP000000598; Chromosome C.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0008301; F:DNA binding, bending; IEA:InterPro.
DR   GO; GO:0045895; P:positive regulation of mating-type specific transcription, DNA-templated; IEA:InterPro.
DR   InterPro; IPR006856; MATalpha_HMGbox.
DR   Pfam; PF04769; MATalpha_HMGbox; 1.
DR   PROSITE; PS51325; ALPHA_BOX; 1.
PE   3: Inferred from homology;
KW   Activator; DNA-binding; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..261
FT                   /note="Mating-type protein ALPHA1"
FT                   /id="PRO_0000206017"
FT   DNA_BIND        101..158
FT                   /note="Alpha box"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00655"
SQ   SEQUENCE   261 AA;  30125 MW;  2203D1496D031161 CRC64;
     MKSNAPTFKV AVSKRSCSSV RKTSKKIRSG MIRKPSVSSR YRKHEGVNLY MSKVTPTSIP
     APPQVLVAYI KEKVKTLNKS EVLMSLGNSN QLSSRDVKTK TKKKQINDFI AFRSYYSRLL
     NGILTQTELS TIISKHWTVD KQTRKNWELI AQEYNCDASG KHFFNWLEVN YGIDKQWLYE
     IVQYEECLTP TTKKPYVENI YNSGFKQRID SPNQKDITFS QSDNSADWLV DPIFFDTEFL
     ATSFDRTNLI ISDCYSLMNS V