ID   MTAL2_KLULA             Reviewed;         223 AA.
AC   Q9HDS5; Q6CV28;
DT   26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   25-MAY-2022, entry version 87.
DE   RecName: Full=Mating-type protein ALPHA2;
DE            Short=MATalpha2 protein;
GN   Name=HMLALPHA2; Synonyms=alpha2; OrderedLocusNames=KLLA0C00374g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=10978277; DOI=10.1093/genetics/156.1.81;
RA   Aastroem S.U., Kegel A., Sjoestrand J.O.O., Rine J.;
RT   "Kluyveromyces lactis Sir2p regulates cation sensitivity and maintains a
RT   specialized chromatin structure at the cryptic alpha-locus.";
RL   Genetics 156:81-91(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Mating type proteins are sequence specific DNA-binding
CC       proteins that act as master switches in yeast differentiation by
CC       controlling gene expression in a cell type-specific fashion.
CC       Transcriptional corepressor that acts in conjunction with A1 to repress
CC       transcription of haploid-specific genes (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Forms a heterodimer with A1. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108}.
CC   -!- MISCELLANEOUS: There are three genetic loci for mating type genes in
CC       K.lactis. MAT is the expression locus that determines the mating type
CC       of the cell, whereas HML (containing HMLALPHA1, HMLALPHA2 and
CC       HMLALPHA3) and HMR (containing HMRA1 and HMRA2) represent silenced
CC       repositories of mating type information. The mating type is determined
CC       by the MAT locus, which contains either a copy of HML or of HMR.
CC       Diploid cells are usually heterozygous for the MAT locus.
CC   -!- SIMILARITY: Belongs to the TALE/M-ATYP homeobox family. {ECO:0000305}.
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DR   EMBL; AF195066; AAG21091.1; -; Genomic_DNA.
DR   EMBL; CR382123; CAH01061.1; -; Genomic_DNA.
DR   RefSeq; XP_452211.1; XM_452211.1.
DR   AlphaFoldDB; Q9HDS5; -.
DR   SMR; Q9HDS5; -.
DR   STRING; 28985.XP_452211.1; -.
DR   PRIDE; Q9HDS5; -.
DR   EnsemblFungi; CAH01061; CAH01061; KLLA0_C00374g.
DR   GeneID; 2892167; -.
DR   KEGG; kla:KLLA0_C00374g; -.
DR   eggNOG; KOG0773; Eukaryota.
DR   HOGENOM; CLU_091806_1_0_1; -.
DR   InParanoid; Q9HDS5; -.
DR   Proteomes; UP000000598; Chromosome C.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   CDD; cd00086; homeodomain; 1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR001356; Homeobox_dom.
DR   Pfam; PF00046; Homeodomain; 1.
DR   SMART; SM00389; HOX; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Homeobox; Nucleus; Reference proteome; Repressor;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..223
FT                   /note="Mating-type protein ALPHA2"
FT                   /id="PRO_0000049180"
FT   DNA_BIND        151..213
FT                   /note="Homeobox; TALE-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT   CONFLICT        159
FT                   /note="L -> P (in Ref. 2; CAH01061)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   223 AA;  26508 MW;  70C5E9998BC39BCC CRC64;
     MSRIPIHSLL NPSESCKSIS NVPSNYRDLS TFNKERAKVI TTFQEMFYSM LENNDDYNKI
     ESLIRNFQPK LTWSHKCESL TFKQKAYLTA IIQKSIKSLL VLLKEKGKMR EIEFSRKEVR
     KINKYRQSSK NFESVNIKIL TQDLMHSNNN EFKKGKRFLK SHIQLLENWY SMNRRNPYLA
     ENDLAYISKN TTLTKTQIKN WLANRRRKDK ITEVSSDIRN ILN