MTA_NOTNE
ID MTA_NOTNE Reviewed; 60 AA.
AC P62339; O73914;
DT 05-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 66.
DE RecName: Full=Metallothionein A;
DE Short=MT-A;
DE AltName: Full=MT_nc;
GN Name=mta;
OS Notothenia neglecta (Yellowbelly rockcod) (Notothenia coriiceps neglecta).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Eupercaria; Perciformes; Notothenioidei; Nototheniidae; Notothenia.
OX NCBI_TaxID=202063;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Liver;
RX PubMed=10406107; DOI=10.1093/oxfordjournals.molbev.a026178;
RA Bargelloni L., Scudiero R., Parisi E., Carginale V., Capasso C.,
RA Patarnello T.;
RT "Metallothioneins in antarctic fish: evidence for independent duplication
RT and gene conversion.";
RL Mol. Biol. Evol. 16:885-897(1999).
RN [2] {ECO:0007744|PDB:1M0G, ECO:0007744|PDB:1M0J}
RP STRUCTURE BY NMR IN COMPLEX WITH CADMIUM IONS.
RX PubMed=12679021; DOI=10.1016/s0969-2126(03)00044-3;
RA Capasso C., Carginale V., Crescenzi O., Di Maro D., Parisi E.,
RA Spadaccini R., Temussi P.A.;
RT "Solution structure of MT_nc, a novel metallothionein from the Antarctic
RT fish Notothenia coriiceps.";
RL Structure 11:435-443(2003).
CC -!- FUNCTION: Metallothioneins have a high content of cysteine residues
CC that bind various heavy metals. {ECO:0000250}.
CC -!- DOMAIN: Class I metallothioneins contain 2 metal-binding domains: four
CC divalent ions are chelated within cluster A of the alpha domain and are
CC coordinated via cysteinyl thiolate bridges to 11 cysteine ligands.
CC Cluster B, the corresponding region within the beta domain, can ligate
CC three divalent ions to 9 cysteines.
CC -!- SIMILARITY: Belongs to the metallothionein superfamily. Type 1 family.
CC {ECO:0000305}.
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DR EMBL; AJ006484; CAA07063.1; -; mRNA.
DR PDB; 1M0G; NMR; -; A=31-60.
DR PDB; 1M0J; NMR; -; A=2-29.
DR PDBsum; 1M0G; -.
DR PDBsum; 1M0J; -.
DR AlphaFoldDB; P62339; -.
DR SMR; P62339; -.
DR EvolutionaryTrace; P62339; -.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR Gene3D; 4.10.10.10; -; 1.
DR InterPro; IPR003019; Metalthion.
DR InterPro; IPR017854; Metalthion_dom_sf.
DR InterPro; IPR023587; Metalthion_dom_sf_vert.
DR InterPro; IPR000006; Metalthion_vert.
DR InterPro; IPR018064; Metalthion_vert_metal_BS.
DR PANTHER; PTHR23299; PTHR23299; 1.
DR Pfam; PF00131; Metallothio; 1.
DR PRINTS; PR00860; MTVERTEBRATE.
DR SUPFAM; SSF57868; SSF57868; 1.
DR PROSITE; PS00203; METALLOTHIONEIN_VRT; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Metal-binding; Metal-thiolate cluster.
FT CHAIN 1..60
FT /note="Metallothionein A"
FT /id="PRO_0000197293"
FT REGION 1..28
FT /note="Beta"
FT REGION 29..60
FT /note="Alpha"
FT BINDING 4
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="1"
FT /ligand_note="in cluster B"
FT /evidence="ECO:0000269|PubMed:12679021,
FT ECO:0007744|PDB:1M0J"
FT BINDING 6
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="1"
FT /ligand_note="in cluster B"
FT /evidence="ECO:0000269|PubMed:12679021,
FT ECO:0007744|PDB:1M0J"
FT BINDING 6
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="2"
FT /ligand_note="in cluster B"
FT /evidence="ECO:0000269|PubMed:12679021,
FT ECO:0007744|PDB:1M0J"
FT BINDING 12
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="2"
FT /ligand_note="in cluster B"
FT /evidence="ECO:0000269|PubMed:12679021,
FT ECO:0007744|PDB:1M0J"
FT BINDING 14
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="2"
FT /ligand_note="in cluster B"
FT /evidence="ECO:0000269|PubMed:12679021,
FT ECO:0007744|PDB:1M0J"
FT BINDING 14
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="3"
FT /ligand_note="in cluster B"
FT /evidence="ECO:0000269|PubMed:12679021,
FT ECO:0007744|PDB:1M0J"
FT BINDING 18
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="3"
FT /ligand_note="in cluster B"
FT /evidence="ECO:0000269|PubMed:12679021,
FT ECO:0007744|PDB:1M0J"
FT BINDING 20
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="1"
FT /ligand_note="in cluster B"
FT /evidence="ECO:0000269|PubMed:12679021,
FT ECO:0007744|PDB:1M0J"
FT BINDING 23
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="1"
FT /ligand_note="in cluster B"
FT /evidence="ECO:0000269|PubMed:12679021,
FT ECO:0007744|PDB:1M0J"
FT BINDING 23
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="3"
FT /ligand_note="in cluster B"
FT /evidence="ECO:0000269|PubMed:12679021,
FT ECO:0007744|PDB:1M0J"
FT BINDING 25
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="2"
FT /ligand_note="in cluster B"
FT /evidence="ECO:0000269|PubMed:12679021,
FT ECO:0007744|PDB:1M0J"
FT BINDING 28
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="3"
FT /ligand_note="in cluster B"
FT /evidence="ECO:0000269|PubMed:12679021,
FT ECO:0007744|PDB:1M0J"
FT BINDING 32
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="4"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:12679021,
FT ECO:0007744|PDB:1M0G"
FT BINDING 33
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="4"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:12679021,
FT ECO:0007744|PDB:1M0G"
FT BINDING 33
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="5"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:12679021,
FT ECO:0007744|PDB:1M0G"
FT BINDING 35
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="5"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:12679021,
FT ECO:0007744|PDB:1M0G"
FT BINDING 36
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="5"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:12679021,
FT ECO:0007744|PDB:1M0G"
FT BINDING 36
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="6"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:12679021,
FT ECO:0007744|PDB:1M0G"
FT BINDING 40
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="6"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:12679021,
FT ECO:0007744|PDB:1M0G"
FT BINDING 43
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="4"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:12679021,
FT ECO:0007744|PDB:1M0G"
FT BINDING 43
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="6"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:12679021,
FT ECO:0007744|PDB:1M0G"
FT BINDING 47
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="4"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:12679021,
FT ECO:0007744|PDB:1M0G"
FT BINDING 49
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="5"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:12679021,
FT ECO:0007744|PDB:1M0G"
FT BINDING 49
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="7"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:12679021,
FT ECO:0007744|PDB:1M0G"
FT BINDING 54
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="7"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:12679021,
FT ECO:0007744|PDB:1M0G"
FT BINDING 58
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="7"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:12679021,
FT ECO:0007744|PDB:1M0G"
FT BINDING 59
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="6"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:12679021,
FT ECO:0007744|PDB:1M0G"
FT BINDING 59
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="7"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:12679021,
FT ECO:0007744|PDB:1M0G"
FT HELIX 5..9
FT /evidence="ECO:0007829|PDB:1M0J"
FT STRAND 15..17
FT /evidence="ECO:0007829|PDB:1M0J"
FT STRAND 20..22
FT /evidence="ECO:0007829|PDB:1M0J"
FT TURN 26..28
FT /evidence="ECO:0007829|PDB:1M0J"
FT HELIX 41..44
FT /evidence="ECO:0007829|PDB:1M0G"
FT HELIX 48..50
FT /evidence="ECO:0007829|PDB:1M0G"
FT STRAND 52..55
FT /evidence="ECO:0007829|PDB:1M0G"
FT TURN 56..59
FT /evidence="ECO:0007829|PDB:1M0G"
SQ SEQUENCE 60 AA; 6019 MW; E866E7155A2C424A CRC64;
MDPCECSKSG TCNCGGSCTC TNCSCKSCKK SCCPCCPSGC TKCASGCVCK GKTCDTSCCQ
