ID   MTB12_MYCTU             Reviewed;         168 AA.
AC   P9WIN7; L0T9F9; O05822; P0A5P8;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 33.
DE   RecName: Full=Low molecular weight antigen MTB12;
DE   AltName: Full=CFP-2;
DE   AltName: Full=Low molecular weight protein antigen 2;
DE   Flags: Precursor;
GN   Name=mtb12; Synonyms=cfp2; OrderedLocusNames=Rv2376c; ORFNames=MTCY27.04;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 49-77.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9712769; DOI=10.1128/iai.66.9.4208-4214.1998;
RA   Webb J.R., Vedvick T.S., Alderson M.R., Guderian J.A., Jen S.S.,
RA   Ovendale P.J., Johnson S.M., Reed S.G., Skeiky Y.A.W.;
RT   "Molecular cloning, expression, and immunogenicity of MTB12, a novel low-
RT   molecular-weight antigen secreted by Mycobacterium tuberculosis.";
RL   Infect. Immun. 66:4208-4214(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [3]
RP   PROTEIN SEQUENCE OF 49-63.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=10812229; DOI=10.1016/s0264-410x(00)00049-9;
RA   Bhaskar S., Khanna S.P., Mukherjee R.;
RT   "Isolation, purification and immunological characterization of novel low
RT   molecular weight protein antigen CFP 6 from culture filtrate of M.
RT   tuberculosis.";
RL   Vaccine 18:2856-2866(2000).
RN   [4]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC   -!- FUNCTION: May play a role in the development of protective immune
CC       responses.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the MTB12 family. {ECO:0000305}.
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DR   EMBL; AF062036; AAC35793.1; -; Genomic_DNA.
DR   EMBL; AL123456; CCP45164.1; -; Genomic_DNA.
DR   PIR; G70587; G70587.
DR   RefSeq; NP_216892.1; NC_000962.3.
DR   RefSeq; WP_003412264.1; NZ_NVQJ01000029.1.
DR   AlphaFoldDB; P9WIN7; -.
DR   STRING; 83332.Rv2376c; -.
DR   PaxDb; P9WIN7; -.
DR   DNASU; 885515; -.
DR   GeneID; 885515; -.
DR   KEGG; mtu:Rv2376c; -.
DR   TubercuList; Rv2376c; -.
DR   eggNOG; ENOG5030PBH; Bacteria.
DR   OMA; FVNQGSW; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005576; C:extracellular region; HDA:MTBBASE.
DR   GO; GO:0009274; C:peptidoglycan-based cell wall; HDA:MTBBASE.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   PROPEP          23..48
FT                   /evidence="ECO:0000269|PubMed:10812229,
FT                   ECO:0000269|PubMed:9712769"
FT                   /id="PRO_0000021777"
FT   CHAIN           49..168
FT                   /note="Low molecular weight antigen MTB12"
FT                   /id="PRO_0000021778"
SQ   SEQUENCE   168 AA;  16635 MW;  A809937458009BFD CRC64;
     MKMVKSIAAG LTAAAAIGAA AAGVTSIMAG GPVVYQMQPV VFGAPLPLDP ASAPDVPTAA
     QLTSLLNSLA DPNVSFANKG SLVEGGIGGT EARIADHKLK KAAEHGDLPL SFSVTNIQPA
     AAGSATADVS VSGPKLSSPV TQNVTFVNQG GWMLSRASAM ELLQAAGN