ID   MTB1_GEOSE              Reviewed;         509 AA.
AC   P70986;
DT   08-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 2.
DT   03-AUG-2022, entry version 78.
DE   RecName: Full=Type II methyltransferase M.BsoBI {ECO:0000303|PubMed:12654995};
DE            Short=M.BsoBI {ECO:0000303|PubMed:12654995};
DE            EC=2.1.1.113 {ECO:0000250|UniProtKB:Q04845};
DE   AltName: Full=Modification methylase BsoBI;
DE   AltName: Full=N(4)- cytosine-specific methyltransferase BsoBI;
GN   Name=bsoBIM {ECO:0000303|PubMed:8917312};
OS   Geobacillus stearothermophilus (Bacillus stearothermophilus).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus.
OX   NCBI_TaxID=1422;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=JN209;
RX   PubMed=8917312; DOI=10.1007/bf02173975;
RA   Ruan H., Lunnen K.D., Scott M.E., Moran L.S., Slatko B.E., Pelletier J.J.,
RA   Hess E.J., Benner J. II, Wilson G.G., Xu S.-Y.;
RT   "Cloning and sequence comparison of AvaI and BsoBI restriction-modification
RT   systems.";
RL   Mol. Gen. Genet. 252:695-699(1996).
RN   [2]
RP   NOMENCLATURE, AND SUBTYPE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: An alpha subtype methylase that recognizes the double-
CC       stranded sequence 5'-CYCGRG-3', methylates C-1 on both strands, and
CC       protects the DNA from cleavage by the BsoBI endonuclease.
CC       {ECO:0000303|PubMed:12654995, ECO:0000305|PubMed:8917312}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = an N(4)-
CC         methyl-2'-deoxycytidine in DNA + H(+) + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:16857, Rhea:RHEA-COMP:11369, Rhea:RHEA-COMP:13674,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:85452, ChEBI:CHEBI:137933; EC=2.1.1.113;
CC         Evidence={ECO:0000250|UniProtKB:Q04845};
CC   -!- SIMILARITY: Belongs to the N(4)/N(6)-methyltransferase family. N(4)
CC       subfamily. {ECO:0000305}.
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DR   EMBL; X98287; CAA66933.1; -; Genomic_DNA.
DR   AlphaFoldDB; P70986; -.
DR   SMR; P70986; -.
DR   REBASE; 3311; M.BsoBI.
DR   BRENDA; 2.1.1.113; 623.
DR   PRO; PR:P70986; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR   GO; GO:0015667; F:site-specific DNA-methyltransferase (cytosine-N4-specific) activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.150; -; 2.
DR   InterPro; IPR002941; DNA_methylase_N4/N6.
DR   InterPro; IPR017985; MeTrfase_CN4_CS.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   Pfam; PF01555; N6_N4_Mtase; 2.
DR   SUPFAM; SSF53335; SSF53335; 2.
DR   PROSITE; PS00093; N4_MTASE; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Methyltransferase; Restriction system;
KW   S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..509
FT                   /note="Type II methyltransferase M.BsoBI"
FT                   /id="PRO_0000087925"
SQ   SEQUENCE   509 AA;  59144 MW;  3C472905C3276D2D CRC64;
     METVQMSLDL QPEDTKANIE CSSPDYEIKC FSHNQFAPQI ERLKIEKKYI HIVEETETFN
     RKLVSYQANK NQSIHNWIRY KEGFSSELVQ NLIEEFGLSK GDTILDPFLG SGTTSLTAKM
     LGINSIGIDI LPISHIAFEP KSFIFEYNLE ELDRAYKEIY EISPTKIEQK FNHLSITEGA
     FPEETENDLL FFTHWDNNSQ YSYQTKTLIK LILVSILEEI SYTRKDGQYL RWDYRSQKVI
     ETNKKRLEQG KEPIKTILDK GELPTVKESL LNTLLTIKED IKEIQQKCLP NESVHELIKD
     SALNALPKIN DNTFDAVITS PPYCNRYDYT RTYALELAYL GVDEKKIREL RQAQLSCTVE
     NKSKLKQLKD YYHSLFLESR YAEIERLVTG NEVLNEINYA LRKRWENGEV NNKGILSMVD
     GYFTELTFIF YELFRTCKPG AKVAFVNDNV RYAGEIIPVD FLSTKIAEDI GFKPIKIYTL
     KQRKGNSSQQ MGKYGRVALR KSITIWEKP