ID   MTB2_BACAM              Reviewed;         265 AA.
AC   P18051;
DT   01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1990, sequence version 1.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=Orphan methyltransferase M.BamHII {ECO:0000303|PubMed:12654995};
DE            Short=M.BamHII {ECO:0000303|PubMed:12654995};
DE            EC=2.1.1.113;
DE   AltName: Full=Modification methylase BamHII;
DE   AltName: Full=N(4)-cytosine-specific methyltransferase BamHII;
GN   Name=bamHIIM;
OS   Bacillus amyloliquefaciens (Bacillus velezensis).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus amyloliquefaciens group.
OX   NCBI_TaxID=1390;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=H;
RX   PubMed=2374727; DOI=10.1093/nar/18.13.4002;
RA   Connaughton J.F., Kaloss W.D., Vanek P.G., Nardone G.A., Chirikjian J.G.;
RT   "The complete sequence of the Bacillus amyloliquefaciens proviral H2, BamHI
RT   methylase gene.";
RL   Nucleic Acids Res. 18:4002-4002(1990).
RN   [2]
RP   NOMENCLATURE, AND SUBTYPE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: A beta subtype methylase, recognizes the double-stranded
CC       sequence 5'-GGATCC-3', methylates C-? on both strands. No endonuclease
CC       has been identified for this methylase, although it is speculated it
CC       might protect against BamHI. {ECO:0000303|PubMed:12654995,
CC       ECO:0000305|PubMed:2374727}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = an N(4)-
CC         methyl-2'-deoxycytidine in DNA + H(+) + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:16857, Rhea:RHEA-COMP:11369, Rhea:RHEA-COMP:13674,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:85452, ChEBI:CHEBI:137933; EC=2.1.1.113;
CC   -!- MISCELLANEOUS: This methylase is encoded on the proviral phage H2.
CC       {ECO:0000269|PubMed:2374727}.
CC   -!- SIMILARITY: Belongs to the N(4)/N(6)-methyltransferase family. N(4)
CC       subfamily. {ECO:0000305}.
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DR   EMBL; X53032; CAA37205.1; -; Genomic_DNA.
DR   PIR; S10316; S10316.
DR   AlphaFoldDB; P18051; -.
DR   SMR; P18051; -.
DR   REBASE; 157607; M2.Rso10709ORF7P.
DR   REBASE; 186; M.BamHII.
DR   REBASE; 204726; M.Bso1395ORF2761P.
DR   BRENDA; 2.1.1.113; 630.
DR   PRO; PR:P18051; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR   GO; GO:0015667; F:site-specific DNA-methyltransferase (cytosine-N4-specific) activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR002941; DNA_methylase_N4/N6.
DR   InterPro; IPR017985; MeTrfase_CN4_CS.
DR   InterPro; IPR001091; RM_Methyltransferase.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   Pfam; PF01555; N6_N4_Mtase; 1.
DR   PRINTS; PR00508; S21N4MTFRASE.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   PROSITE; PS00093; N4_MTASE; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Methyltransferase; Restriction system;
KW   S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..265
FT                   /note="Orphan methyltransferase M.BamHII"
FT                   /id="PRO_0000087921"
SQ   SEQUENCE   265 AA;  30981 MW;  895A51217A814820 CRC64;
     MKENIGDCTI DLTVTSPPYD DLRNYNGYSF NFEETAQELY RVTKEGGVVV WVVGDKTHKG
     SETGSSFRQA LYFKELGFNL HDTMIYEKDS ISFPDKNRYY QIFEYMFIFS KGKPKTINLL
     ADRKNKWYNG KKHIKGHYRK MDGEKVRHHK QNLLKEFGVR FNIWRIPNGH QKSTLDKIAF
     QHPAIFPEKL AEDHILSWSN EGDIVFDPFM GSGTTAKMAA LNNRKYIGTE ISKEYCDIAN
     ERLKNYIILH KRMEGKGYRL PQVHS