ID   MTBB1_METBF             Reviewed;         467 AA.
AC   P0C0W5;
DT   10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT   26-FEB-2008, sequence version 2.
DT   25-MAY-2022, entry version 54.
DE   RecName: Full=Dimethylamine methyltransferase MtbB1;
DE            Short=DMA methyltransferase 1;
DE            Short=DMAMT 1;
DE            EC=2.1.1.249;
DE   AltName: Full=Dimethylamine--corrinoid protein methyltransferase 1;
GN   Name=mtbB1; OrderedLocusNames=Mbar_A1506;
OS   Methanosarcina barkeri (strain Fusaro / DSM 804).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=269797;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Fusaro / DSM 804;
RX   PubMed=16980466; DOI=10.1128/jb.00810-06;
RA   Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S.,
RA   Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.;
RT   "The Methanosarcina barkeri genome: comparative analysis with
RT   Methanosarcina acetivorans and Methanosarcina mazei reveals extensive
RT   rearrangement within methanosarcinal genomes.";
RL   J. Bacteriol. 188:7922-7931(2006).
RN   [2]
RP   PROTEIN SEQUENCE OF 2-17, FUNCTION, CATALYTIC ACTIVITY, AND
RP   BIOPHYSICOCHEMICAL PROPERTIES.
RC   STRAIN=Fusaro / DSM 804;
RX   PubMed=9654067; DOI=10.1046/j.1432-1327.1998.2530692.x;
RA   Wassenaar R.W., Keltjens J.T., van der Drift C., Vogels G.D.;
RT   "Purification and characterization of dimethylamine:5-
RT   hydroxybenzimidazolylcobamide methyltransferase from Methanosarcina barkeri
RT   Fusaro.";
RL   Eur. J. Biochem. 253:692-697(1998).
CC   -!- FUNCTION: Catalyzes the transfer of a methyl group from dimethylamine
CC       to the corrinoid cofactor of MtbC. {ECO:0000269|PubMed:9654067}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Co(I)-[dimethylamine-specific corrinoid protein] +
CC         dimethylamine + H(+) = methyl-Co(III)-[dimethylamine-specific
CC         corrinoid protein] + methylamine; Xref=Rhea:RHEA:41175, Rhea:RHEA-
CC         COMP:11122, Rhea:RHEA-COMP:11123, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:58040, ChEBI:CHEBI:59338, ChEBI:CHEBI:85033,
CC         ChEBI:CHEBI:85035; EC=2.1.1.249;
CC         Evidence={ECO:0000269|PubMed:9654067};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=0.45 mM for dimethylamine {ECO:0000269|PubMed:9654067};
CC         KM=4.5 mM for monomethylamine {ECO:0000269|PubMed:9654067};
CC   -!- PATHWAY: One-carbon metabolism; methanogenesis from dimethylamine.
CC   -!- SIMILARITY: Belongs to the dimethylamine methyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; CP000099; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   UniPathway; UPA00644; -.
DR   GO; GO:0043791; F:dimethylamine methyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0015948; P:methanogenesis; IEA:UniProtKB-KW.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   InterPro; IPR012653; Dimeth_MeTrfase_MtbB.
DR   Pfam; PF09505; Dimeth_Pyl; 1.
DR   TIGRFAMs; TIGR02368; dimeth_PyL; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Methanogenesis; Methyltransferase; Pyrrolysine;
KW   Transferase.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:9654067"
FT   CHAIN           2..467
FT                   /note="Dimethylamine methyltransferase MtbB1"
FT                   /id="PRO_0000216561"
FT   NON_STD         356
FT                   /note="Pyrrolysine"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        16
FT                   /note="Y -> K (in Ref. 2; AA sequence)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   467 AA;  50311 MW;  AD08B1C0AF6F1BDF CRC64;
     MATEYALRMG DGKRIYLTKE KIIAEIEDGT ANAADLGEIP ALNANEMEKL AEILMMPGKT
     VSVEQGMEVP VTHDIGTIRL DGDQGNSGVG IPSSRLVGCM THERAFGADT MELGHIDYSF
     KPVKPVVSNE CQAMEVCQQN MIIPLFYGAM PNMGLYYTPD GPFENPGDLM KAFKIPEAWE
     SMEHAAEHLT RDTVWVMQKL FASGADGVNF DTTGAAGDGD MYGTLHAIEA LRKEFPDMYI
     EAGMAGECVL GMHGNLQYDG VTLAGLWPHQ QAPLVAKAGA NVFGPVCNTN TSKTSAWNLA
     RAVTFMKAAV EASPIPCHVD MGMGVGGIPM LETPPIDAVT RASKAMVEIA GVDGIOIGVG
     DPMGMPIAHI MASGMTGMRA AGDLVARMEF SKNMRIGEAK EYVAKKLGVD QMDLVDEHVM
     RELREELDIG IITSVPGAAK GIAAKMNIEK LLDIKINSCN LFRKQIA