ID   MTBB2_METAC             Reviewed;         468 AA.
AC   Q8TS72;
DT   26-JUL-2002, integrated into UniProtKB/Swiss-Prot.
DT   26-FEB-2008, sequence version 3.
DT   23-FEB-2022, entry version 84.
DE   RecName: Full=Dimethylamine methyltransferase MtbB2;
DE            Short=DMA methyltransferase 2;
DE            Short=DMAMT 2;
DE            EC=2.1.1.249;
DE   AltName: Full=Dimethylamine--corrinoid protein methyltransferase 2;
GN   Name=mtbB2; OrderedLocusNames=MA_0933;
OS   Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 /
OS   C2A).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=188937;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35395 / DSM 2834 / JCM 12185 / C2A;
RX   PubMed=11932238; DOI=10.1101/gr.223902;
RA   Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W.,
RA   Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J.,
RA   Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P.,
RA   McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D.,
RA   Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R.,
RA   Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J.,
RA   Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A.,
RA   White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H.,
RA   Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V.,
RA   Zinder S.H., Lander E., Metcalf W.W., Birren B.;
RT   "The genome of Methanosarcina acetivorans reveals extensive metabolic and
RT   physiological diversity.";
RL   Genome Res. 12:532-542(2002).
CC   -!- FUNCTION: Catalyzes the transfer of a methyl group from dimethylamine
CC       to the corrinoid cofactor of MtbC. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Co(I)-[dimethylamine-specific corrinoid protein] +
CC         dimethylamine + H(+) = methyl-Co(III)-[dimethylamine-specific
CC         corrinoid protein] + methylamine; Xref=Rhea:RHEA:41175, Rhea:RHEA-
CC         COMP:11122, Rhea:RHEA-COMP:11123, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:58040, ChEBI:CHEBI:59338, ChEBI:CHEBI:85033,
CC         ChEBI:CHEBI:85035; EC=2.1.1.249;
CC   -!- PATHWAY: One-carbon metabolism; methanogenesis from dimethylamine.
CC   -!- SIMILARITY: Belongs to the dimethylamine methyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; AE010299; AAM04366.1; -; Genomic_DNA.
DR   STRING; 188937.MA_0933; -.
DR   KEGG; mac:MA_0933; -.
DR   HOGENOM; CLU_046512_0_0_2; -.
DR   OMA; VVLTYDI; -.
DR   PhylomeDB; Q8TS72; -.
DR   UniPathway; UPA00644; -.
DR   Proteomes; UP000002487; Chromosome.
DR   GO; GO:0043791; F:dimethylamine methyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0015948; P:methanogenesis; IEA:UniProtKB-KW.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   InterPro; IPR012653; Dimeth_MeTrfase_MtbB.
DR   Pfam; PF09505; Dimeth_Pyl; 1.
DR   TIGRFAMs; TIGR02368; dimeth_PyL; 1.
PE   3: Inferred from homology;
KW   Methanogenesis; Methyltransferase; Pyrrolysine; Reference proteome;
KW   Transferase.
FT   CHAIN           1..468
FT                   /note="Dimethylamine methyltransferase MtbB2"
FT                   /id="PRO_0000216564"
FT   NON_STD         356
FT                   /note="Pyrrolysine"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   468 AA;  50575 MW;  4A9D40A6CBF19477 CRC64;
     MATEYALRMG DGKRIFLTKE KIREELEAGA ANAADLGEIP ALSGDEIDRL AEILMMPGKA
     VSVEQGMEVP VTHDIGTIRL DGDQGNSGVG IPSSRLVGCM MHERAFGADT MELGHIDYSF
     KPVKPVVSNE CQAMEVCQQN MIIPLFYGAM PNMGLYYTPD GPFENPGDLM KAFKIQEAWE
     SMEHAADHLT RDTVWIMQKL FASGADGVNF DTTAAAGDGD FYGTLHAVEA LRKEFPAMHI
     EVGMAGEMVL GMHGNLQYEG VTLAGLWPHQ QASLVAKAGA NVFGPVVNTN TSKTSPWNLA
     RAVTFIKEAV KVSSLPCHVD MGMGVGGIPM LETPPIDAVT RASKAMVEVA GVDGIOIGVG
     DPLGMPISHI MASGMTGIRA AGDLVARMQF SKNMRIKEAK KYVAKKLDVD VMDLADEHVM
     RELREELDIG VITSVPGAAK GIAAKMNIEK LLDVRINSCE LFRKQTGR