ID   MTBB3_METAC             Reviewed;         467 AA.
AC   Q8TN68;
DT   26-JUL-2002, integrated into UniProtKB/Swiss-Prot.
DT   26-FEB-2008, sequence version 3.
DT   23-FEB-2022, entry version 81.
DE   RecName: Full=Dimethylamine methyltransferase MtbB3;
DE            Short=DMA methyltransferase 3;
DE            Short=DMAMT 3;
DE            EC=2.1.1.249;
DE   AltName: Full=Dimethylamine--corrinoid protein methyltransferase 3;
GN   Name=mtbB3; OrderedLocusNames=MA_2425;
OS   Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 /
OS   C2A).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=188937;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35395 / DSM 2834 / JCM 12185 / C2A;
RX   PubMed=11932238; DOI=10.1101/gr.223902;
RA   Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W.,
RA   Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J.,
RA   Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P.,
RA   McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D.,
RA   Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R.,
RA   Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J.,
RA   Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A.,
RA   White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H.,
RA   Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V.,
RA   Zinder S.H., Lander E., Metcalf W.W., Birren B.;
RT   "The genome of Methanosarcina acetivorans reveals extensive metabolic and
RT   physiological diversity.";
RL   Genome Res. 12:532-542(2002).
CC   -!- FUNCTION: Catalyzes the transfer of a methyl group from dimethylamine
CC       to the corrinoid cofactor of MtbC. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Co(I)-[dimethylamine-specific corrinoid protein] +
CC         dimethylamine + H(+) = methyl-Co(III)-[dimethylamine-specific
CC         corrinoid protein] + methylamine; Xref=Rhea:RHEA:41175, Rhea:RHEA-
CC         COMP:11122, Rhea:RHEA-COMP:11123, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:58040, ChEBI:CHEBI:59338, ChEBI:CHEBI:85033,
CC         ChEBI:CHEBI:85035; EC=2.1.1.249;
CC   -!- PATHWAY: One-carbon metabolism; methanogenesis from dimethylamine.
CC   -!- SIMILARITY: Belongs to the dimethylamine methyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; AE010299; AAM05811.1; -; Genomic_DNA.
DR   STRING; 188937.MA_2425; -.
DR   KEGG; mac:MA_2425; -.
DR   HOGENOM; CLU_046512_0_0_2; -.
DR   OMA; KINSCET; -.
DR   PhylomeDB; Q8TN68; -.
DR   UniPathway; UPA00644; -.
DR   Proteomes; UP000002487; Chromosome.
DR   GO; GO:0043791; F:dimethylamine methyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0015948; P:methanogenesis; IEA:UniProtKB-KW.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   InterPro; IPR012653; Dimeth_MeTrfase_MtbB.
DR   Pfam; PF09505; Dimeth_Pyl; 1.
DR   TIGRFAMs; TIGR02368; dimeth_PyL; 1.
PE   3: Inferred from homology;
KW   Methanogenesis; Methyltransferase; Pyrrolysine; Reference proteome;
KW   Transferase.
FT   CHAIN           1..467
FT                   /note="Dimethylamine methyltransferase MtbB3"
FT                   /id="PRO_0000216565"
FT   NON_STD         356
FT                   /note="Pyrrolysine"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   467 AA;  50414 MW;  0419882C4214052B CRC64;
     MATEYALRMG DGKRIYLTKE KILSEIEAGS SNAADLGDIP DLSTDEMKKL AEILMMPGKA
     VSVEQGMEVP VTHDIGTIRL DGDQGNSGVG IPSSRLVGCM THERAFGADT MELGHIDYSF
     KPVKPVVSNE CQAMEVCQQN MIIPLFYGAM PNMGLYYTPD GPFENPGDLM KMFKIDKARE
     SMEHAADHLT RDTVWVMQKL FASGADGVNF DTTAAAGDAD MYGTLRAVEV LRAQFPEMYI
     EVGMAGEMVL GMHGELEYDE VRLAGLWPHE QAPLIAKAGA NVFGPVVNTN TSKTSAWNLA
     RAVTFIKEAV KASPIPCHVN MGMGVGGIPM LETPPVDAVT RASKAMVEVA GVDGIOIGVG
     DPLGMPISHI MASGMTGIRA AGDLVARMEF SKNMRIGEAK EYVAKKLNVD TMDLADEHVM
     RELREELDIG VITSVPGAAK GIAAKMNIEK LLGIKINSCE TFRAQLA