MTC1_PBCVI
ID MTC1_PBCVI Reviewed; 367 AA.
AC P36216;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1994, sequence version 1.
DT 03-AUG-2022, entry version 81.
DE RecName: Full=Type II methyltransferase M.CviJI {ECO:0000303|PubMed:12654995};
DE Short=M.CviJI {ECO:0000303|PubMed:2158687};
DE EC=2.1.1.37;
DE AltName: Full=Cytosine-specific methyltransferase CviJI;
DE AltName: Full=Modification methylase CviJI;
GN Name=CVIJIM {ECO:0000303|PubMed:2158687};
OS Paramecium bursaria Chlorella virus IL3A (PBCV-IL3A).
OC Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes;
OC Algavirales; Phycodnaviridae; Chlorovirus.
OX NCBI_TaxID=46019;
OH NCBI_TaxID=3071; Chlorella.
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2158687; DOI=10.1016/0042-6822(90)90225-g;
RA Shields S.L., Burbank D.E., Grabherr R., van Etten J.L.;
RT "Cloning and sequencing the cytosine methyltransferase gene M. CviJI from
RT Chlorella virus IL-3A.";
RL Virology 176:16-24(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 357-367, AND FUNCTION.
RX PubMed=8692682; DOI=10.1093/nar/24.13.2463;
RA Swaminathan N., Mead D.A., McMaster K., George D., van Etten J.L.,
RA Skowron P.M.;
RT "Molecular cloning of the three base restriction endonuclease R.CviJI from
RT eukaryotic Chlorella virus IL-3A.";
RL Nucleic Acids Res. 24:2463-2469(1996).
RN [3]
RP NOMENCLATURE.
RX PubMed=12654995; DOI=10.1093/nar/gkg274;
RA Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT endonucleases and their genes.";
RL Nucleic Acids Res. 31:1805-1812(2003).
CC -!- FUNCTION: A methylase that recognizes the double-stranded sequence 5'-
CC RGCY-3', methylates C-3 on both strands, and protects the DNA from
CC cleavage by the CviJI endonuclease. {ECO:0000269|PubMed:8692682,
CC ECO:0000303|PubMed:12654995}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = a 5-
CC methyl-2'-deoxycytidine in DNA + H(+) + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:13681, Rhea:RHEA-COMP:11369, Rhea:RHEA-COMP:11370,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:85452, ChEBI:CHEBI:85454; EC=2.1.1.37;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10018};
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. C5-methyltransferase family. {ECO:0000255|PROSITE-
CC ProRule:PRU01016}.
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DR EMBL; M27265; AAA88826.1; -; Genomic_DNA.
DR EMBL; U09001; AAC55063.1; -; Genomic_DNA.
DR PIR; A46355; A46355.
DR SMR; P36216; -.
DR PRO; PR:P36216; -.
DR GO; GO:0003886; F:DNA (cytosine-5-)-methyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.150; -; 1.
DR InterPro; IPR018117; C5_DNA_meth_AS.
DR InterPro; IPR001525; C5_MeTfrase.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR Pfam; PF00145; DNA_methylase; 1.
DR PRINTS; PR00105; C5METTRFRASE.
DR SUPFAM; SSF53335; SSF53335; 1.
DR TIGRFAMs; TIGR00675; dcm; 1.
DR PROSITE; PS00094; C5_MTASE_1; 1.
DR PROSITE; PS51679; SAM_MT_C5; 1.
PE 3: Inferred from homology;
KW DNA-binding; Methyltransferase; Restriction system;
KW S-adenosyl-L-methionine; Transferase.
FT CHAIN 1..367
FT /note="Type II methyltransferase M.CviJI"
FT /id="PRO_0000087871"
FT DOMAIN 3..367
FT /note="SAM-dependent MTase C5-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01016"
FT ACT_SITE 73
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01016,
FT ECO:0000255|PROSITE-ProRule:PRU10018"
SQ SEQUENCE 367 AA; 41861 MW; 15BD56B0354E2ECC CRC64;
MSFRTLELFA GIAGISHGLR GISTPVAFVE INEDAQKFLK TKFSDASVFN DVTKFTKSDF
PEDIDMITAG FPCTGFSIAG SRTGFEHKES GLFADVVRIT EEYKPKIVFL ENSHMLSHTY
NLDVVVKKMD EIGYFCKWVT CRASIIGAHH QRHRWFCLAI RKDYEPEEII VSVNATKFDW
ENNEPPCQVD NKSYENSTLV RLAGYSVVPD QIRYAFTGLF TGDFESSWKT TLTPGTIIGT
EHKKMKGTYD KVINGYYEND VYYSFSRKEV HRAPLNISVK PRDIPEKHNG KTLVDREMIK
KYWCTPCASY GTATAGCNVL TDRQSHALPT QVRFSYRGVC GRHLSGIWCA WLMGYDQEYL
GYLVQYD
