ID   MTC3_PBCVC              Reviewed;         377 AA.
AC   P10835;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=Modification methylase CviBIII;
DE            Short=M.CviBIII {ECO:0000303|PubMed:3320956};
DE            EC=2.1.1.72;
DE   AltName: Full=Adenine-specific methyltransferase CviBIII;
DE   AltName: Full=Orphan methyltransferase M.CviBIII {ECO:0000303|PubMed:12654995};
GN   Name=CVIBIIIM;
OS   Paramecium bursaria Chlorella virus NC1A (PBCV-NC1A).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes;
OC   Algavirales; Phycodnaviridae; Chlorovirus.
OX   NCBI_TaxID=46020;
OH   NCBI_TaxID=3071; Chlorella.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=3320956; DOI=10.1093/nar/15.23.9807;
RA   Narva K.E., Wendell D.L., Skrdla M.P., van Etten J.L.;
RT   "Molecular cloning and characterization of the gene encoding the DNA
RT   methyltransferase, M.CviBIII, from Chlorella virus NC-1A.";
RL   Nucleic Acids Res. 15:9807-9823(1987).
RN   [2]
RP   NOMENCLATURE, AND SUBTYPE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: A gamma subtype methylase that recognizes the double-stranded
CC       sequence 5'-TCGA-3' and methylates A-4 on both strands.
CC       {ECO:0000269|PubMed:3320956, ECO:0000303|PubMed:12654995}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC         N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC         homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC         COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC   -!- DISRUPTION PHENOTYPE: Not essential for viral replication it can be
CC       deleted. {ECO:0000269|PubMed:3320956}.
CC   -!- SIMILARITY: Belongs to the N(4)/N(6)-methyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; X06618; CAA29835.1; -; Genomic_DNA.
DR   SMR; P10835; -.
DR   REBASE; 752; M.CviBIII.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR   GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR003356; DNA_methylase_A-5.
DR   InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   Pfam; PF02384; N6_Mtase; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   PROSITE; PS00092; N6_MTASE; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Methyltransferase; S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..377
FT                   /note="Modification methylase CviBIII"
FT                   /id="PRO_0000087948"
SQ   SEQUENCE   377 AA;  42895 MW;  B22D471367D61BBC CRC64;
     MNVEQYTQVT TDFEKTLTKE KKSKQGIFFT PKTVREKLFG FTEHFQNTPG FSILEPSCGT
     GEIISECVER FPLASIKGVE LDNDMSTICS KKYAEYNVDI VNEDFLLWKG GKFDFIVGNP
     PYVVRPSGYK NDNRIAKGRS NLYVEFLYKC ITEHLKEDGI LAFIIPSTIG NSSFYEPIRK
     LIITLDILSF EILDKHDFCD TNTRLCSIVI KNSPGTGKYT YRDYICDKDI PHHGNSYIGS
     LDLKFKTGFA WANVNKFFTD KSEIPFFTSS NIKLNEIHIG DKMKYLTQDT TKFFTGKALL
     IKTASAGKRG GRFEFGFSLY ENDKWAVDND IIVIQGPDTV LSIVQDVLMK DVTNEFINIL
     VNNGHISMKL LKSIPLF