MTCA1_MYCBO
ID MTCA1_MYCBO Reviewed; 163 AA.
AC P64798; A0A1R3XXW3; Q10612; X2BHA3;
DT 11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 84.
DE RecName: Full=Beta-carbonic anhydrase 1 {ECO:0000305};
DE Short=Beta-CA 1 {ECO:0000305};
DE EC=4.2.1.1 {ECO:0000250|UniProtKB:P9WPJ7};
DE AltName: Full=Carbonate dehydratase 1 {ECO:0000305};
DE AltName: Full=mtCA 1 {ECO:0000305};
GN OrderedLocusNames=BQ2027_MB1315;
OS Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=233413;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-935 / AF2122/97;
RX PubMed=12788972; DOI=10.1073/pnas.1130426100;
RA Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M.,
RA Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B.,
RA Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J.,
RA Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G.;
RT "The complete genome sequence of Mycobacterium bovis.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=ATCC BAA-935 / AF2122/97;
RX PubMed=28385856; DOI=10.1128/genomea.00157-17;
RA Malone K.M., Farrell D., Stuber T.P., Schubert O.T., Aebersold R.,
RA Robbe-Austerman S., Gordon S.V.;
RT "Updated reference genome sequence and annotation of Mycobacterium bovis
RT AF2122/97.";
RL Genome Announc. 5:E00157-E00157(2017).
CC -!- FUNCTION: Catalyzes the reversible hydration of carbon dioxide to form
CC bicarbonate. {ECO:0000250|UniProtKB:P9WPJ7}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H(+) + hydrogencarbonate = CO2 + H2O; Xref=Rhea:RHEA:10748,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526,
CC ChEBI:CHEBI:17544; EC=4.2.1.1;
CC Evidence={ECO:0000250|UniProtKB:P9WPJ7};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000250|UniProtKB:P9WPJ7};
CC Note=Binds 1 zinc ion per subunit. {ECO:0000250|UniProtKB:P9WPJ7};
CC -!- SUBUNIT: Homotetramer. {ECO:0000250|UniProtKB:P9WPJ7}.
CC -!- SIMILARITY: Belongs to the beta-class carbonic anhydrase family.
CC {ECO:0000305}.
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DR EMBL; LT708304; SIT99918.1; -; Genomic_DNA.
DR RefSeq; NP_854969.1; NC_002945.3.
DR RefSeq; WP_003406616.1; NC_002945.4.
DR AlphaFoldDB; P64798; -.
DR SMR; P64798; -.
DR EnsemblBacteria; SIT99918; SIT99918; BQ2027_MB1315.
DR GeneID; 45425256; -.
DR PATRIC; fig|233413.5.peg.1441; -.
DR OMA; AIFTCMD; -.
DR Proteomes; UP000001419; Chromosome.
DR GO; GO:0004089; F:carbonate dehydratase activity; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR Gene3D; 3.40.1050.10; -; 1.
DR InterPro; IPR001765; Carbonic_anhydrase.
DR InterPro; IPR036874; Carbonic_anhydrase_sf.
DR PANTHER; PTHR43175; PTHR43175; 1.
DR Pfam; PF00484; Pro_CA; 1.
DR SMART; SM00947; Pro_CA; 1.
DR SUPFAM; SSF53056; SSF53056; 1.
PE 3: Inferred from homology;
KW Lyase; Metal-binding; Zinc.
FT CHAIN 1..163
FT /note="Beta-carbonic anhydrase 1"
FT /id="PRO_0000103785"
FT BINDING 35
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:P9WPJ7"
FT BINDING 37
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:P9WPJ7"
FT BINDING 88
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:P9WPJ7"
FT BINDING 91
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:P9WPJ7"
SQ SEQUENCE 163 AA; 18189 MW; DDD342B7DBFE5AA0 CRC64;
MTVTDDYLAN NVDYASGFKG PLPMPPSKHI AIVACMDARL DVYRMLGIKE GEAHVIRNAG
CVVTDDVIRS LAISQRLLGT REIILLHHTD CGMLTFTDDD FKRAIQDETG IRPTWSPESY
PDAVEDVRQS LRRIEVNPFV TKHTSLRGFV FDVATGKLNE VTP
