ID   MTCD_TETTH              Reviewed;         162 AA.
AC   Q8WSW3; Q5XQF6; Q86BU0; Q8IT78;
DT   16-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   25-MAY-2022, entry version 62.
DE   RecName: Full=Cadmium metallothionein;
DE   AltName: Full=Cd-MT;
DE   AltName: Full=MT-Cd;
DE   Flags: Precursor;
GN   Name=MTT1;
OS   Tetrahymena thermophila.
OC   Eukaryota; Sar; Alveolata; Ciliophora; Intramacronucleata;
OC   Oligohymenophorea; Hymenostomatida; Tetrahymenina; Tetrahymenidae;
OC   Tetrahymena.
OX   NCBI_TaxID=5911;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND INDUCTION.
RC   STRAIN=CU428;
RX   PubMed=11891286; DOI=10.1073/pnas.052016199;
RA   Shang Y., Song X., Bowen J., Corstanje R., Gao Y., Gaertig J.,
RA   Gorovsky M.A.;
RT   "A robust inducible-repressible promoter greatly facilitates gene
RT   knockouts, conditional expression, and overexpression of homologous and
RT   heterologous genes in Tetrahymena thermophila.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:3734-3739(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC   STRAIN=Inbred strain B, and SB1969;
RX   PubMed=17356700; DOI=10.1371/journal.pone.0000291;
RA   Diaz S., Amaro F., Rico D., Campos V., Benitez L., Martin-Gonzalez A.,
RA   Hamilton E.P., Orias E., Gutierrez J.C.;
RT   "Tetrahymena metallothioneins fall into two discrete subfamilies.";
RL   PLoS ONE 2:E291-E291(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Inbred B;
RA   Formigari A., Santovito G., Boldrin F., Irato P., Piccinni E.;
RT   "Evolution of metallothioneins in Tetrahymena thermophila.";
RL   Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 2-162.
RA   Miao W., Wan M., Song Y.;
RT   "Tetrahymena thermophila cadmium metallothionein gene.";
RL   Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 7-162, PROTEIN SEQUENCE OF 3-17, INDUCTION,
RP   AND MASS SPECTROMETRY.
RC   STRAIN=CU428;
RX   PubMed=15305793; DOI=10.1078/143446104774199565;
RA   Dondero F., Cavaletto M., Ghezzi A.R., La Terza A., Banni M., Viarengo A.;
RT   "Biochemical characterization and quantitative gene expression analysis of
RT   the multi-stress inducible metallothionein from Tetrahymena thermophila.";
RL   Protist 155:157-168(2004).
CC   -!- FUNCTION: The metallothioneins are involved in the cellular
CC       sequestration of toxic metal ions.
CC   -!- INDUCTION: By cadmium, and to a lesser extent by mercury, copper, heat,
CC       hydrogen peroxide and IL-6. {ECO:0000269|PubMed:11891286,
CC       ECO:0000269|PubMed:15305793}.
CC   -!- MASS SPECTROMETRY: Mass=16763; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:15305793};
CC   -!- SIMILARITY: Belongs to the metallothionein superfamily. Type 7 family.
CC       {ECO:0000305}.
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DR   EMBL; AY061892; AAL31645.1; -; Genomic_DNA.
DR   EMBL; AY740525; AAU87360.1; -; Genomic_DNA.
DR   EMBL; DQ517937; ABF68755.1; -; mRNA.
DR   EMBL; EF195745; ABN42648.1; -; mRNA.
DR   EMBL; DQ022186; AAY42865.1; -; mRNA.
DR   EMBL; AY273793; AAP33500.1; -; mRNA.
DR   EMBL; AF537326; AAN06822.1; -; mRNA.
DR   AlphaFoldDB; Q8WSW3; -.
DR   OMA; CCSQTCC; -.
DR   GO; GO:0046870; F:cadmium ion binding; IEA:InterPro.
DR   InterPro; IPR012484; Metalthion_7.
DR   Pfam; PF07846; Metallothio_Cad; 3.
PE   1: Evidence at protein level;
KW   Cadmium; Direct protein sequencing; Metal-binding; Metal-thiolate cluster.
FT   PROPEP          1..2
FT                   /evidence="ECO:0000269|PubMed:15305793"
FT                   /id="PRO_0000018673"
FT   CHAIN           3..162
FT                   /note="Cadmium metallothionein"
FT                   /id="PRO_0000018674"
FT   CONFLICT        6
FT                   /note="S -> NN (in Ref. 4; AAP33500)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        156
FT                   /note="Q -> K (in Ref. 4; AAP33500)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        159
FT                   /note="Q -> E (in Ref. 4; AAP33500)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   162 AA;  16757 MW;  9B2DB3C95981FFCD CRC64;
     MDKVNSCCCG VNAKPCCTDP NSGCCCVSKT DNCCKSDTKE CCTGTGEGCK CVNCKCCKPQ
     ANCCCGVNAK PCCFDPNSGC CCVSKTNNCC KSDTKECCTG TGEGCKCTSC QCCKPVQQGC
     CCGDKAKACC TDPNSGCCCS NKANKCCDAT SKQECQTCQC CK