ID   MTDTR_PSEAE             Reviewed;         110 AA.
AC   Q9HUH5;
DT   19-MAR-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 105.
DE   RecName: Full=Multidrug transporter PA4990;
GN   OrderedLocusNames=PA4990;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
RN   [2]
RP   FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, AND SUBCELLULAR LOCATION.
RX   PubMed=11574548; DOI=10.1074/jbc.m108231200;
RA   Ninio S., Rotem D., Schuldiner S.;
RT   "Functional analysis of novel multidrug transporters from human
RT   pathogens.";
RL   J. Biol. Chem. 276:48250-48256(2001).
CC   -!- FUNCTION: Confers resistance to ethidium bromide, acriflavine and
CC       methyl viologen. {ECO:0000269|PubMed:11574548}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=2426 uM for methyl viologen {ECO:0000269|PubMed:11574548};
CC         Vmax=4800 nmol/min/mg enzyme with methyl viologen as substrate
CC         {ECO:0000269|PubMed:11574548};
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:11574548};
CC       Multi-pass membrane protein {ECO:0000269|PubMed:11574548}.
CC   -!- SIMILARITY: Belongs to the drug/metabolite transporter (DMT)
CC       superfamily. Small multidrug resistance (SMR) (TC 2.A.7.1) family.
CC       {ECO:0000305}.
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DR   EMBL; AE004091; AAG08375.1; -; Genomic_DNA.
DR   PIR; C83021; C83021.
DR   RefSeq; NP_253677.1; NC_002516.2.
DR   RefSeq; WP_003095735.1; NZ_QZGE01000002.1.
DR   AlphaFoldDB; Q9HUH5; -.
DR   SMR; Q9HUH5; -.
DR   STRING; 287.DR97_2344; -.
DR   PaxDb; Q9HUH5; -.
DR   DNASU; 880147; -.
DR   EnsemblBacteria; AAG08375; AAG08375; PA4990.
DR   GeneID; 880147; -.
DR   KEGG; pae:PA4990; -.
DR   PATRIC; fig|208964.12.peg.5230; -.
DR   PseudoCAP; PA4990; -.
DR   HOGENOM; CLU_133067_0_2_6; -.
DR   InParanoid; Q9HUH5; -.
DR   OMA; ATTAMKY; -.
DR   PhylomeDB; Q9HUH5; -.
DR   BioCyc; PAER208964:G1FZ6-5106-MON; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR   GO; GO:0015199; F:amino-acid betaine transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0015297; F:antiporter activity; IBA:GO_Central.
DR   GO; GO:0015220; F:choline transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0015871; P:choline transport; IBA:GO_Central.
DR   GO; GO:0031460; P:glycine betaine transport; IBA:GO_Central.
DR   GO; GO:0042221; P:response to chemical; IEA:UniProt.
DR   GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR   InterPro; IPR000390; Small_drug/metabolite_transptr.
DR   InterPro; IPR045324; Small_multidrug_res.
DR   PANTHER; PTHR30561; PTHR30561; 1.
DR   Pfam; PF00893; Multi_Drug_Res; 1.
PE   1: Evidence at protein level;
KW   Antiport; Cell membrane; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..110
FT                   /note="Multidrug transporter PA4990"
FT                   /id="PRO_0000425956"
FT   TRANSMEM        7..27
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        31..51
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        58..78
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        85..105
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   110 AA;  11489 MW;  95ED027B31DF52FE CRC64;
     MTNYLYLAIA IAAEVVATTS LKAVAGFSKP LPLLLVVGGY VLAFSMLVLV MRTLPVGVVY
     AIWSGLGIVL VSLVAMFVYG QRLDPAALLG IGLIIAGVLV IQLFSRASGH