ID   MTD_ARCFU               Reviewed;         273 AA.
AC   O29544; Q9UWM0;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   03-AUG-2022, entry version 119.
DE   RecName: Full=F420-dependent methylenetetrahydromethanopterin dehydrogenase;
DE            Short=MTD;
DE            EC=1.5.98.1;
DE   AltName: Full=Coenzyme F420-dependent N5,N10-methylenetetrahydromethanopterin dehydrogenase;
GN   Name=mtd; OrderedLocusNames=AF_0714;
OS   Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC
OS   100126 / VC-16).
OC   Archaea; Euryarchaeota; Archaeoglobi; Archaeoglobales; Archaeoglobaceae;
OC   Archaeoglobus.
OX   NCBI_TaxID=224325;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16;
RX   PubMed=9389475; DOI=10.1038/37052;
RA   Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A.,
RA   Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L.,
RA   Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D.,
RA   Quackenbush J., Lee N.H., Sutton G.G., Gill S.R., Kirkness E.F.,
RA   Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N.,
RA   Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R.,
RA   Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D.,
RA   Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P.,
RA   Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M.,
RA   Smith H.O., Woese C.R., Venter J.C.;
RT   "The complete genome sequence of the hyperthermophilic, sulphate-reducing
RT   archaeon Archaeoglobus fulgidus.";
RL   Nature 390:364-370(1997).
RN   [2]
RP   PROTEIN SEQUENCE OF 2-34, AND CHARACTERIZATION.
RC   STRAIN=ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16;
RX   PubMed=8481089; DOI=10.1007/bf00248476;
RA   Schworer B., Breitung J., Klein A.R., Stetter K.O., Thauer R.K.;
RT   "Formylmethanofuran: tetrahydromethanopterin formyltransferase and N5,N10-
RT   methylenetetrahydromethanopterin dehydrogenase from the sulfate-reducing
RT   Archaeoglobus fulgidus: similarities with the enzymes from methanogenic
RT   Archaea.";
RL   Arch. Microbiol. 159:225-232(1993).
CC   -!- FUNCTION: Catalyzes the oxidation of methylene-H(4)MPT to methenyl-
CC       H(4)MPT(+).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5,10-methylenetetrahydromethanopterin + 2 H(+) + oxidized
CC         coenzyme F420-(gamma-L-Glu)(n) = 5,10-methenyl-5,6,7,8-
CC         tetrahydromethanopterin + reduced coenzyme F420-(gamma-L-Glu)(n);
CC         Xref=Rhea:RHEA:16721, Rhea:RHEA-COMP:12939, Rhea:RHEA-COMP:14378,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57818, ChEBI:CHEBI:58337,
CC         ChEBI:CHEBI:133980, ChEBI:CHEBI:139511; EC=1.5.98.1;
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Optimum pH is about 5.5.;
CC       Temperature dependence:
CC         Optimum temperature is 70 degrees Celsius. Thermostable up to 90
CC         degrees Celsius, however, only in the presence of salts.;
CC   -!- PATHWAY: Metabolic intermediate metabolism; lactate oxidation.
CC   -!- SIMILARITY: Belongs to the MTD family. {ECO:0000305}.
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DR   EMBL; AE000782; AAB90526.1; -; Genomic_DNA.
DR   PIR; B69339; B69339.
DR   RefSeq; WP_010878217.1; NC_000917.1.
DR   AlphaFoldDB; O29544; -.
DR   SMR; O29544; -.
DR   STRING; 224325.AF_0714; -.
DR   PRIDE; O29544; -.
DR   EnsemblBacteria; AAB90526; AAB90526; AF_0714.
DR   GeneID; 24794314; -.
DR   KEGG; afu:AF_0714; -.
DR   eggNOG; arCOG04382; Archaea.
DR   HOGENOM; CLU_1006890_0_0_2; -.
DR   OMA; KGCFMTK; -.
DR   OrthoDB; 46875at2157; -.
DR   PhylomeDB; O29544; -.
DR   BioCyc; MetaCyc:AF_RS03625-MON; -.
DR   UniPathway; UPA00701; -.
DR   Proteomes; UP000002199; Chromosome.
DR   GO; GO:0008901; F:ferredoxin hydrogenase activity; IEA:InterPro.
DR   GO; GO:0030268; F:methylenetetrahydromethanopterin dehydrogenase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0019516; P:lactate oxidation; IEA:UniProtKB-UniPathway.
DR   GO; GO:0015948; P:methanogenesis; IEA:InterPro.
DR   GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00058; MTD; 1.
DR   InterPro; IPR002844; MTD.
DR   InterPro; IPR036080; MTD_sf.
DR   Pfam; PF01993; MTD; 1.
DR   PIRSF; PIRSF005627; MTD; 1.
DR   SUPFAM; SSF102324; SSF102324; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; One-carbon metabolism; Oxidoreductase;
KW   Reference proteome.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:8481089"
FT   CHAIN           2..273
FT                   /note="F420-dependent methylenetetrahydromethanopterin
FT                   dehydrogenase"
FT                   /id="PRO_0000075036"
SQ   SEQUENCE   273 AA;  29645 MW;  335B2432D5FE7B7E CRC64;
     MVVKVGVLKM GAIGTALLVE YLLDERADRE DIEVRVVTSG AKMQPEEAVV AEKLKEFDPD
     VVIVVSPNAA LPGPKAAREA FEGKPVIVIS DAPAKKAKDE LKEKGFGYIL INADSMIGAR
     REFLDPTEMA LFNADVVKVL AATGAFRLVQ EAIDKVIEDI KAGKQPELPQ IVVTAEKAVE
     AGKFSNPYAK AKAMAAFYIA EKVADIDVKG CFIEKDPEKY IPLVASAHEM MRIAAILADQ
     AREIEKSNDT VFRNPHAKDG KILGKTQLMA KPE