ID   MTD_METAC               Reviewed;         279 AA.
AC   Q8THT2;
DT   28-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   25-MAY-2022, entry version 98.
DE   RecName: Full=F420-dependent methylenetetrahydromethanopterin dehydrogenase;
DE            Short=MTD;
DE            EC=1.5.98.1;
DE   AltName: Full=Coenzyme F420-dependent N5,N10-methylenetetrahydromethanopterin dehydrogenase;
GN   Name=mtd; OrderedLocusNames=MA_4430;
OS   Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 /
OS   C2A).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=188937;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35395 / DSM 2834 / JCM 12185 / C2A;
RX   PubMed=11932238; DOI=10.1101/gr.223902;
RA   Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W.,
RA   Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J.,
RA   Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P.,
RA   McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D.,
RA   Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R.,
RA   Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J.,
RA   Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A.,
RA   White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H.,
RA   Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V.,
RA   Zinder S.H., Lander E., Metcalf W.W., Birren B.;
RT   "The genome of Methanosarcina acetivorans reveals extensive metabolic and
RT   physiological diversity.";
RL   Genome Res. 12:532-542(2002).
CC   -!- FUNCTION: Catalyzes the reversible reduction of methenyl-H(4)MPT(+) to
CC       methylene-H(4)MPT. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5,10-methylenetetrahydromethanopterin + 2 H(+) + oxidized
CC         coenzyme F420-(gamma-L-Glu)(n) = 5,10-methenyl-5,6,7,8-
CC         tetrahydromethanopterin + reduced coenzyme F420-(gamma-L-Glu)(n);
CC         Xref=Rhea:RHEA:16721, Rhea:RHEA-COMP:12939, Rhea:RHEA-COMP:14378,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57818, ChEBI:CHEBI:58337,
CC         ChEBI:CHEBI:133980, ChEBI:CHEBI:139511; EC=1.5.98.1;
CC   -!- PATHWAY: One-carbon metabolism; methanogenesis from CO(2); 5,10-
CC       methylene-5,6,7,8-tetrahydromethanopterin from 5,10-methenyl-5,6,7,8-
CC       tetrahydromethanopterin (coenzyme F420 route): step 1/1.
CC   -!- SIMILARITY: Belongs to the MTD family. {ECO:0000305}.
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DR   EMBL; AE010299; AAM07771.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q8THT2; -.
DR   SMR; Q8THT2; -.
DR   STRING; 188937.MA_4430; -.
DR   EnsemblBacteria; AAM07771; AAM07771; MA_4430.
DR   KEGG; mac:MA_4430; -.
DR   HOGENOM; CLU_1006890_0_0_2; -.
DR   InParanoid; Q8THT2; -.
DR   OMA; KGCFMTK; -.
DR   PhylomeDB; Q8THT2; -.
DR   UniPathway; UPA00640; UER00695.
DR   Proteomes; UP000002487; Chromosome.
DR   GO; GO:0008901; F:ferredoxin hydrogenase activity; IEA:InterPro.
DR   GO; GO:0030268; F:methylenetetrahydromethanopterin dehydrogenase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0019386; P:methanogenesis, from carbon dioxide; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00058; MTD; 1.
DR   InterPro; IPR002844; MTD.
DR   InterPro; IPR036080; MTD_sf.
DR   Pfam; PF01993; MTD; 1.
DR   PIRSF; PIRSF005627; MTD; 1.
DR   SUPFAM; SSF102324; SSF102324; 1.
PE   3: Inferred from homology;
KW   Methanogenesis; One-carbon metabolism; Oxidoreductase; Reference proteome.
FT   CHAIN           1..279
FT                   /note="F420-dependent methylenetetrahydromethanopterin
FT                   dehydrogenase"
FT                   /id="PRO_0000075037"
SQ   SEQUENCE   279 AA;  30268 MW;  2AB2991081836567 CRC64;
     MRKMVNIGFI KMGNLGMSQV INLIQDEIAA REGITVRVFG TGAKMGPADA ADTESFKQWN
     ADFVVMISPN AAAPGPTAAR EIWKDVPCIV VSDGPTKKEA REALEQEGFG YIILPVDPLI
     GAKREFLDPV EMASFNSDAM KVLSSCGVVR LIQEELDRVT EQVASGKSGE DLELPHIFAK
     PEKCVEHAGF ANPYAKAKAL AALHMAEKVA QVNFPACFML KEVEQVCLTA AAGHEIMGAA
     AILATQAREI EKSNDTVFRQ PHAKNGTLLK KVKLYEKPE