ID   MTD_METLZ               Reviewed;         280 AA.
AC   A2SPK1;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   06-MAR-2007, sequence version 1.
DT   25-MAY-2022, entry version 74.
DE   RecName: Full=F420-dependent methylenetetrahydromethanopterin dehydrogenase {ECO:0000255|HAMAP-Rule:MF_00058};
DE            Short=MTD {ECO:0000255|HAMAP-Rule:MF_00058};
DE            EC=1.5.98.1 {ECO:0000255|HAMAP-Rule:MF_00058};
DE   AltName: Full=Coenzyme F420-dependent N5,N10-methylenetetrahydromethanopterin dehydrogenase {ECO:0000255|HAMAP-Rule:MF_00058};
GN   Name=mtd {ECO:0000255|HAMAP-Rule:MF_00058}; OrderedLocusNames=Mlab_0079;
OS   Methanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
OX   NCBI_TaxID=410358;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43576 / DSM 4855 / Z;
RX   PubMed=21304657; DOI=10.4056/sigs.35575;
RA   Anderson I.J., Sieprawska-Lupa M., Goltsman E., Lapidus A., Copeland A.,
RA   Glavina Del Rio T., Tice H., Dalin E., Barry K., Pitluck S., Hauser L.,
RA   Land M., Lucas S., Richardson P., Whitman W.B., Kyrpides N.C.;
RT   "Complete genome sequence of Methanocorpusculum labreanum type strain Z.";
RL   Stand. Genomic Sci. 1:197-203(2009).
CC   -!- FUNCTION: Catalyzes the reversible reduction of methenyl-H(4)MPT(+) to
CC       methylene-H(4)MPT. {ECO:0000255|HAMAP-Rule:MF_00058}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5,10-methylenetetrahydromethanopterin + 2 H(+) + oxidized
CC         coenzyme F420-(gamma-L-Glu)(n) = 5,10-methenyl-5,6,7,8-
CC         tetrahydromethanopterin + reduced coenzyme F420-(gamma-L-Glu)(n);
CC         Xref=Rhea:RHEA:16721, Rhea:RHEA-COMP:12939, Rhea:RHEA-COMP:14378,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57818, ChEBI:CHEBI:58337,
CC         ChEBI:CHEBI:133980, ChEBI:CHEBI:139511; EC=1.5.98.1;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00058};
CC   -!- PATHWAY: One-carbon metabolism; methanogenesis from CO(2); 5,10-
CC       methylene-5,6,7,8-tetrahydromethanopterin from 5,10-methenyl-5,6,7,8-
CC       tetrahydromethanopterin (coenzyme F420 route): step 1/1.
CC       {ECO:0000255|HAMAP-Rule:MF_00058}.
CC   -!- SIMILARITY: Belongs to the MTD family. {ECO:0000255|HAMAP-
CC       Rule:MF_00058}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CP000559; ABN06257.1; -; Genomic_DNA.
DR   RefSeq; WP_011832458.1; NC_008942.1.
DR   AlphaFoldDB; A2SPK1; -.
DR   SMR; A2SPK1; -.
DR   STRING; 410358.Mlab_0079; -.
DR   EnsemblBacteria; ABN06257; ABN06257; Mlab_0079.
DR   GeneID; 4794919; -.
DR   KEGG; mla:Mlab_0079; -.
DR   eggNOG; arCOG04382; Archaea.
DR   HOGENOM; CLU_1006890_0_0_2; -.
DR   OMA; KGCFMTK; -.
DR   OrthoDB; 46875at2157; -.
DR   UniPathway; UPA00640; UER00695.
DR   Proteomes; UP000000365; Chromosome.
DR   GO; GO:0008901; F:ferredoxin hydrogenase activity; IEA:InterPro.
DR   GO; GO:0030268; F:methylenetetrahydromethanopterin dehydrogenase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0019386; P:methanogenesis, from carbon dioxide; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00058; MTD; 1.
DR   InterPro; IPR002844; MTD.
DR   InterPro; IPR036080; MTD_sf.
DR   Pfam; PF01993; MTD; 1.
DR   SUPFAM; SSF102324; SSF102324; 1.
PE   3: Inferred from homology;
KW   Methanogenesis; One-carbon metabolism; Oxidoreductase; Reference proteome.
FT   CHAIN           1..280
FT                   /note="F420-dependent methylenetetrahydromethanopterin
FT                   dehydrogenase"
FT                   /id="PRO_1000117811"
SQ   SEQUENCE   280 AA;  30248 MW;  F5110D817376E765 CRC64;
     MVVKVGVAKL GNIAAGVMAE LLLDERADRE DMMTFMATSG TKLQKEDVDR VVSNLKAWQP
     DFAIVVSPNG VLEGPVGARE DLKAAGIPTI VITDDVTTKK EGWEALKASG FGYIIVKADA
     MIGARREFLD PVEMADYNGN LVKVLALTGA FRKLQLELDK VIDQVKAGKK GAELELPKLV
     LNSDNSTKGE FTNPYALAKA RAAYEIAQAV AGVNVKGCFM TKEWTDYVPI VASAHEMMRA
     AANLCDQARE LEKGCDGVIR KPHKKTGEIV SKVALISKPE