MTD_METMA
ID MTD_METMA Reviewed; 279 AA.
AC Q8PXV8;
DT 28-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 25-MAY-2022, entry version 109.
DE RecName: Full=F420-dependent methylenetetrahydromethanopterin dehydrogenase;
DE Short=MTD;
DE EC=1.5.98.1;
DE AltName: Full=Coenzyme F420-dependent N5,N10-methylenetetrahydromethanopterin dehydrogenase;
GN Name=mtd; OrderedLocusNames=MM_1108;
OS Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM
OS 11833 / OCM 88) (Methanosarcina frisia).
OC Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX NCBI_TaxID=192952;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88;
RX PubMed=12125824;
RA Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A.,
RA Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C.,
RA Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S.,
RA Bhattacharyya A., Lykidis A., Overbeek R., Klenk H.-P., Gunsalus R.P.,
RA Fritz H.-J., Gottschalk G.;
RT "The genome of Methanosarcina mazei: evidence for lateral gene transfer
RT between Bacteria and Archaea.";
RL J. Mol. Microbiol. Biotechnol. 4:453-461(2002).
CC -!- FUNCTION: Catalyzes the reversible reduction of methenyl-H(4)MPT(+) to
CC methylene-H(4)MPT. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=5,10-methylenetetrahydromethanopterin + 2 H(+) + oxidized
CC coenzyme F420-(gamma-L-Glu)(n) = 5,10-methenyl-5,6,7,8-
CC tetrahydromethanopterin + reduced coenzyme F420-(gamma-L-Glu)(n);
CC Xref=Rhea:RHEA:16721, Rhea:RHEA-COMP:12939, Rhea:RHEA-COMP:14378,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57818, ChEBI:CHEBI:58337,
CC ChEBI:CHEBI:133980, ChEBI:CHEBI:139511; EC=1.5.98.1;
CC -!- PATHWAY: One-carbon metabolism; methanogenesis from CO(2); 5,10-
CC methylene-5,6,7,8-tetrahydromethanopterin from 5,10-methenyl-5,6,7,8-
CC tetrahydromethanopterin (coenzyme F420 route): step 1/1.
CC -!- SIMILARITY: Belongs to the MTD family. {ECO:0000305}.
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DR EMBL; AE008384; AAM30804.1; -; Genomic_DNA.
DR AlphaFoldDB; Q8PXV8; -.
DR SMR; Q8PXV8; -.
DR STRING; 192952.MM_1108; -.
DR EnsemblBacteria; AAM30804; AAM30804; MM_1108.
DR KEGG; mma:MM_1108; -.
DR PATRIC; fig|192952.21.peg.1297; -.
DR eggNOG; arCOG04382; Archaea.
DR HOGENOM; CLU_1006890_0_0_2; -.
DR OMA; KGCFMTK; -.
DR UniPathway; UPA00640; UER00695.
DR Proteomes; UP000000595; Chromosome.
DR GO; GO:0008901; F:ferredoxin hydrogenase activity; IEA:InterPro.
DR GO; GO:0030268; F:methylenetetrahydromethanopterin dehydrogenase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0019386; P:methanogenesis, from carbon dioxide; IEA:UniProtKB-UniPathway.
DR GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-UniRule.
DR HAMAP; MF_00058; MTD; 1.
DR InterPro; IPR002844; MTD.
DR InterPro; IPR036080; MTD_sf.
DR Pfam; PF01993; MTD; 1.
DR PIRSF; PIRSF005627; MTD; 1.
DR SUPFAM; SSF102324; SSF102324; 1.
PE 3: Inferred from homology;
KW Methanogenesis; One-carbon metabolism; Oxidoreductase; Reference proteome.
FT CHAIN 1..279
FT /note="F420-dependent methylenetetrahydromethanopterin
FT dehydrogenase"
FT /id="PRO_0000075040"
SQ SEQUENCE 279 AA; 30240 MW; F52747A8B98723DE CRC64;
MKKMVNIGFI KMGNLGMSQV INLIQDEIAA REGITVRVFG TGAKMAPADA ADTESFKQWN
ADFVVIISPN AAAPGPTAAR EIWKDVPCII VSDGPTKKEA REAFEQEGFG YIILPVDPLI
GAKREFLDSV EMASFNADAM KVLSICGVVR LIQEELDKVT EQVASGKSGK DLELPHIFAK
PEKCVEHAGF ANPYAKSKAL AALHMAEKVA QVNFPACFML KEIEQVCLTA AAGHEIMGAA
AQLANQAREI EKSNDTVSRM PHAKNGAVLK KVRLYEKPE
