ID   MTD_METMJ               Reviewed;         280 AA.
AC   A3CSZ5;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   20-MAR-2007, sequence version 1.
DT   25-MAY-2022, entry version 73.
DE   RecName: Full=F420-dependent methylenetetrahydromethanopterin dehydrogenase {ECO:0000255|HAMAP-Rule:MF_00058};
DE            Short=MTD {ECO:0000255|HAMAP-Rule:MF_00058};
DE            EC=1.5.98.1 {ECO:0000255|HAMAP-Rule:MF_00058};
DE   AltName: Full=Coenzyme F420-dependent N5,N10-methylenetetrahydromethanopterin dehydrogenase {ECO:0000255|HAMAP-Rule:MF_00058};
GN   Name=mtd {ECO:0000255|HAMAP-Rule:MF_00058}; OrderedLocusNames=Memar_0562;
OS   Methanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
OX   NCBI_TaxID=368407;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35101 / DSM 1498 / JR1;
RX   PubMed=21304656; DOI=10.4056/sigs.32535;
RA   Anderson I.J., Sieprawska-Lupa M., Lapidus A., Nolan M., Copeland A.,
RA   Glavina Del Rio T., Tice H., Dalin E., Barry K., Saunders E., Han C.,
RA   Brettin T., Detter J.C., Bruce D., Mikhailova N., Pitluck S., Hauser L.,
RA   Land M., Lucas S., Richardson P., Whitman W.B., Kyrpides N.C.;
RT   "Complete genome sequence of Methanoculleus marisnigri Romesser et al. 1981
RT   type strain JR1.";
RL   Stand. Genomic Sci. 1:189-196(2009).
CC   -!- FUNCTION: Catalyzes the reversible reduction of methenyl-H(4)MPT(+) to
CC       methylene-H(4)MPT. {ECO:0000255|HAMAP-Rule:MF_00058}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5,10-methylenetetrahydromethanopterin + 2 H(+) + oxidized
CC         coenzyme F420-(gamma-L-Glu)(n) = 5,10-methenyl-5,6,7,8-
CC         tetrahydromethanopterin + reduced coenzyme F420-(gamma-L-Glu)(n);
CC         Xref=Rhea:RHEA:16721, Rhea:RHEA-COMP:12939, Rhea:RHEA-COMP:14378,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57818, ChEBI:CHEBI:58337,
CC         ChEBI:CHEBI:133980, ChEBI:CHEBI:139511; EC=1.5.98.1;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00058};
CC   -!- PATHWAY: One-carbon metabolism; methanogenesis from CO(2); 5,10-
CC       methylene-5,6,7,8-tetrahydromethanopterin from 5,10-methenyl-5,6,7,8-
CC       tetrahydromethanopterin (coenzyme F420 route): step 1/1.
CC       {ECO:0000255|HAMAP-Rule:MF_00058}.
CC   -!- SIMILARITY: Belongs to the MTD family. {ECO:0000255|HAMAP-
CC       Rule:MF_00058}.
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DR   EMBL; CP000562; ABN56495.1; -; Genomic_DNA.
DR   RefSeq; WP_011843405.1; NC_009051.1.
DR   AlphaFoldDB; A3CSZ5; -.
DR   SMR; A3CSZ5; -.
DR   STRING; 368407.Memar_0562; -.
DR   EnsemblBacteria; ABN56495; ABN56495; Memar_0562.
DR   GeneID; 4848258; -.
DR   KEGG; mem:Memar_0562; -.
DR   eggNOG; arCOG04382; Archaea.
DR   HOGENOM; CLU_1006890_0_0_2; -.
DR   OMA; KGCFMTK; -.
DR   OrthoDB; 46875at2157; -.
DR   UniPathway; UPA00640; UER00695.
DR   Proteomes; UP000002146; Chromosome.
DR   GO; GO:0008901; F:ferredoxin hydrogenase activity; IEA:InterPro.
DR   GO; GO:0030268; F:methylenetetrahydromethanopterin dehydrogenase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0019386; P:methanogenesis, from carbon dioxide; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00058; MTD; 1.
DR   InterPro; IPR002844; MTD.
DR   InterPro; IPR036080; MTD_sf.
DR   Pfam; PF01993; MTD; 1.
DR   PIRSF; PIRSF005627; MTD; 1.
DR   SUPFAM; SSF102324; SSF102324; 1.
PE   3: Inferred from homology;
KW   Methanogenesis; One-carbon metabolism; Oxidoreductase.
FT   CHAIN           1..280
FT                   /note="F420-dependent methylenetetrahydromethanopterin
FT                   dehydrogenase"
FT                   /id="PRO_1000117812"
SQ   SEQUENCE   280 AA;  30454 MW;  9E05A290FF5C2815 CRC64;
     MVVKVGIAKL GNIASGVMGE LLLDERADRE DMITFMATSG TKLQPEDIER VVSNMKAWGP
     DFCIVVSPNG VLPGPTQARE DLAAAGIPCI VITDDVTTKK EQFEALKASN FGYIIMKADA
     MIGARREFLD PIEMADYNGN LVKVLALTGA FRKMQMELDK VIDQVKAGKK GADLALPKVV
     MTSDKAVDGE FSNPYALAKA RAAYEIAQSV AGVNVKGCFM TKEWEKYIPI VASAHEMMRQ
     AMLLCEEART LEKGVDAVIR KPHKKTGEIV SKTALISKPE