MTD_METPE
ID MTD_METPE Reviewed; 280 AA.
AC B8GJQ7;
DT 28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 03-MAR-2009, sequence version 1.
DT 25-MAY-2022, entry version 58.
DE RecName: Full=F420-dependent methylenetetrahydromethanopterin dehydrogenase {ECO:0000255|HAMAP-Rule:MF_00058};
DE Short=MTD {ECO:0000255|HAMAP-Rule:MF_00058};
DE EC=1.5.98.1 {ECO:0000255|HAMAP-Rule:MF_00058};
DE AltName: Full=Coenzyme F420-dependent N5,N10-methylenetetrahydromethanopterin dehydrogenase {ECO:0000255|HAMAP-Rule:MF_00058};
GN Name=mtd {ECO:0000255|HAMAP-Rule:MF_00058}; OrderedLocusNames=Mpal_0329;
OS Methanosphaerula palustris (strain ATCC BAA-1556 / DSM 19958 / E1-9c).
OC Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC Methanomicrobiales; Methanoregulaceae; Methanosphaerula.
OX NCBI_TaxID=521011;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-1556 / DSM 19958 / E1-9c;
RX PubMed=26543115; DOI=10.1128/genomea.01280-15;
RA Cadillo-Quiroz H., Browne P., Kyrpides N., Woyke T., Goodwin L., Detter C.,
RA Yavitt J.B., Zinder S.H.;
RT "Complete Genome Sequence of Methanosphaerula palustris E1-9CT, a
RT Hydrogenotrophic Methanogen Isolated from a Minerotrophic Fen Peatland.";
RL Genome Announc. 3:0-0(2015).
CC -!- FUNCTION: Catalyzes the reversible reduction of methenyl-H(4)MPT(+) to
CC methylene-H(4)MPT. {ECO:0000255|HAMAP-Rule:MF_00058}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=5,10-methylenetetrahydromethanopterin + 2 H(+) + oxidized
CC coenzyme F420-(gamma-L-Glu)(n) = 5,10-methenyl-5,6,7,8-
CC tetrahydromethanopterin + reduced coenzyme F420-(gamma-L-Glu)(n);
CC Xref=Rhea:RHEA:16721, Rhea:RHEA-COMP:12939, Rhea:RHEA-COMP:14378,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57818, ChEBI:CHEBI:58337,
CC ChEBI:CHEBI:133980, ChEBI:CHEBI:139511; EC=1.5.98.1;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00058};
CC -!- PATHWAY: One-carbon metabolism; methanogenesis from CO(2); 5,10-
CC methylene-5,6,7,8-tetrahydromethanopterin from 5,10-methenyl-5,6,7,8-
CC tetrahydromethanopterin (coenzyme F420 route): step 1/1.
CC {ECO:0000255|HAMAP-Rule:MF_00058}.
CC -!- SIMILARITY: Belongs to the MTD family. {ECO:0000255|HAMAP-
CC Rule:MF_00058}.
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DR EMBL; CP001338; ACL15711.1; -; Genomic_DNA.
DR RefSeq; WP_012617030.1; NC_011832.1.
DR AlphaFoldDB; B8GJQ7; -.
DR SMR; B8GJQ7; -.
DR STRING; 521011.Mpal_0329; -.
DR EnsemblBacteria; ACL15711; ACL15711; Mpal_0329.
DR GeneID; 7272630; -.
DR KEGG; mpl:Mpal_0329; -.
DR eggNOG; arCOG04382; Archaea.
DR HOGENOM; CLU_1006890_0_0_2; -.
DR OMA; KGCFMTK; -.
DR OrthoDB; 46875at2157; -.
DR UniPathway; UPA00640; UER00695.
DR Proteomes; UP000002457; Chromosome.
DR GO; GO:0008901; F:ferredoxin hydrogenase activity; IEA:InterPro.
DR GO; GO:0030268; F:methylenetetrahydromethanopterin dehydrogenase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0019386; P:methanogenesis, from carbon dioxide; IEA:UniProtKB-UniPathway.
DR GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-UniRule.
DR HAMAP; MF_00058; MTD; 1.
DR InterPro; IPR002844; MTD.
DR InterPro; IPR036080; MTD_sf.
DR Pfam; PF01993; MTD; 1.
DR PIRSF; PIRSF005627; MTD; 1.
DR SUPFAM; SSF102324; SSF102324; 1.
PE 3: Inferred from homology;
KW Methanogenesis; One-carbon metabolism; Oxidoreductase; Reference proteome.
FT CHAIN 1..280
FT /note="F420-dependent methylenetetrahydromethanopterin
FT dehydrogenase"
FT /id="PRO_1000117813"
SQ SEQUENCE 280 AA; 30351 MW; 3CF335D70A3BCE57 CRC64;
MVVKVGIAKL GNIASGVMAE LLLDERADRE DMQTFMATSG TKLQPEDIDR VVSTMKAWEP
DFCIVVSPNG VLPGPVGARD ELLKAGIPCV VITDDITTKK EGWEALKASS FGYIIMKADA
MIGARREFLD PVEMADFNGN LVKVLALTGA FRKLQTELDK VIDQVKEGKK GADLVLPKVV
MTSDKAVDGE FSNPYALAKA RAAYEIAQAV AGVNVKGCFM TKEWEKYIPI VASAHEMMRQ
AAALGDEARE IEKAGNGIIR KPHKKDGTIV SKITLISKPE
