MTD_METS3
ID MTD_METS3 Reviewed; 275 AA.
AC A5UMI1;
DT 28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 10-JUL-2007, sequence version 1.
DT 25-MAY-2022, entry version 69.
DE RecName: Full=F420-dependent methylenetetrahydromethanopterin dehydrogenase {ECO:0000255|HAMAP-Rule:MF_00058};
DE Short=MTD {ECO:0000255|HAMAP-Rule:MF_00058};
DE EC=1.5.98.1 {ECO:0000255|HAMAP-Rule:MF_00058};
DE AltName: Full=Coenzyme F420-dependent N5,N10-methylenetetrahydromethanopterin dehydrogenase {ECO:0000255|HAMAP-Rule:MF_00058};
GN Name=mtd {ECO:0000255|HAMAP-Rule:MF_00058}; OrderedLocusNames=Msm_1204;
OS Methanobrevibacter smithii (strain ATCC 35061 / DSM 861 / OCM 144 / PS).
OC Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC Methanobacteriales; Methanobacteriaceae; Methanobrevibacter.
OX NCBI_TaxID=420247;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 35061 / DSM 861 / OCM 144 / PS;
RX PubMed=17563350; DOI=10.1073/pnas.0704189104;
RA Samuel B.S., Hansen E.E., Manchester J.K., Coutinho P.M., Henrissat B.,
RA Fulton R., Latreille P., Kim K., Wilson R.K., Gordon J.I.;
RT "Genomic and metabolic adaptations of Methanobrevibacter smithii to the
RT human gut.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:10643-10648(2007).
CC -!- FUNCTION: Catalyzes the reversible reduction of methenyl-H(4)MPT(+) to
CC methylene-H(4)MPT. {ECO:0000255|HAMAP-Rule:MF_00058}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=5,10-methylenetetrahydromethanopterin + 2 H(+) + oxidized
CC coenzyme F420-(gamma-L-Glu)(n) = 5,10-methenyl-5,6,7,8-
CC tetrahydromethanopterin + reduced coenzyme F420-(gamma-L-Glu)(n);
CC Xref=Rhea:RHEA:16721, Rhea:RHEA-COMP:12939, Rhea:RHEA-COMP:14378,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57818, ChEBI:CHEBI:58337,
CC ChEBI:CHEBI:133980, ChEBI:CHEBI:139511; EC=1.5.98.1;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00058};
CC -!- PATHWAY: One-carbon metabolism; methanogenesis from CO(2); 5,10-
CC methylene-5,6,7,8-tetrahydromethanopterin from 5,10-methenyl-5,6,7,8-
CC tetrahydromethanopterin (coenzyme F420 route): step 1/1.
CC {ECO:0000255|HAMAP-Rule:MF_00058}.
CC -!- SIMILARITY: Belongs to the MTD family. {ECO:0000255|HAMAP-
CC Rule:MF_00058}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP000678; ABQ87409.1; -; Genomic_DNA.
DR RefSeq; WP_011954353.1; NC_009515.1.
DR AlphaFoldDB; A5UMI1; -.
DR SMR; A5UMI1; -.
DR STRING; 420247.Msm_1204; -.
DR PRIDE; A5UMI1; -.
DR EnsemblBacteria; ABQ87409; ABQ87409; Msm_1204.
DR GeneID; 5217296; -.
DR KEGG; msi:Msm_1204; -.
DR PATRIC; fig|420247.28.peg.1203; -.
DR eggNOG; arCOG04382; Archaea.
DR HOGENOM; CLU_1006890_0_0_2; -.
DR OMA; KGCFMTK; -.
DR BRENDA; 1.5.98.1; 11234.
DR UniPathway; UPA00640; UER00695.
DR Proteomes; UP000001992; Chromosome.
DR GO; GO:0008901; F:ferredoxin hydrogenase activity; IEA:InterPro.
DR GO; GO:0030268; F:methylenetetrahydromethanopterin dehydrogenase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0019386; P:methanogenesis, from carbon dioxide; IEA:UniProtKB-UniPathway.
DR GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-UniRule.
DR HAMAP; MF_00058; MTD; 1.
DR InterPro; IPR002844; MTD.
DR InterPro; IPR036080; MTD_sf.
DR Pfam; PF01993; MTD; 1.
DR PIRSF; PIRSF005627; MTD; 1.
DR SUPFAM; SSF102324; SSF102324; 1.
PE 3: Inferred from homology;
KW Methanogenesis; One-carbon metabolism; Oxidoreductase.
FT CHAIN 1..275
FT /note="F420-dependent methylenetetrahydromethanopterin
FT dehydrogenase"
FT /id="PRO_1000117814"
SQ SEQUENCE 275 AA; 29231 MW; 44A26F8351D6EEDD CRC64;
MVVKIGIIKS GNIGTSPVLD LLLDERADRP NIDVRVFGSG AKMNPEQVED VVPKVDQFDP
DFCIFISPNP GAPGPAKARE LLSEKDIPAI IIGDAPGKGK KDEMDEQGLG YIIVMSDPMI
GAKREWLDPT EMAIFNADIL KVLAETGALR LVQNTIDGVI DGAAAGNIEL PKLIITAEKA
VEAAGFENPY AKAKAIAAYE MAGAVANLDM KGCFMTKGFE NFIPLVAAAH EMAASAAALA
DEAREIEKGN DSVLRTPHMK EGNTGCKTDL ISKPE
