ID   MTD_METST               Reviewed;         276 AA.
AC   Q2NHQ2;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   07-FEB-2006, sequence version 1.
DT   25-MAY-2022, entry version 72.
DE   RecName: Full=F420-dependent methylenetetrahydromethanopterin dehydrogenase {ECO:0000255|HAMAP-Rule:MF_00058};
DE            Short=MTD {ECO:0000255|HAMAP-Rule:MF_00058};
DE            EC=1.5.98.1 {ECO:0000255|HAMAP-Rule:MF_00058};
DE   AltName: Full=Coenzyme F420-dependent N5,N10-methylenetetrahydromethanopterin dehydrogenase {ECO:0000255|HAMAP-Rule:MF_00058};
GN   Name=mtd {ECO:0000255|HAMAP-Rule:MF_00058}; OrderedLocusNames=Msp_0163;
OS   Methanosphaera stadtmanae (strain ATCC 43021 / DSM 3091 / JCM 11832 /
OS   MCB-3).
OC   Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC   Methanobacteriales; Methanobacteriaceae; Methanosphaera.
OX   NCBI_TaxID=339860;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3;
RX   PubMed=16385054; DOI=10.1128/jb.188.2.642-658.2006;
RA   Fricke W.F., Seedorf H., Henne A., Kruer M., Liesegang H., Hedderich R.,
RA   Gottschalk G., Thauer R.K.;
RT   "The genome sequence of Methanosphaera stadtmanae reveals why this human
RT   intestinal archaeon is restricted to methanol and H2 for methane formation
RT   and ATP synthesis.";
RL   J. Bacteriol. 188:642-658(2006).
CC   -!- FUNCTION: Catalyzes the oxidation of methylene-H(4)MPT to methenyl-
CC       H(4)MPT(+). {ECO:0000255|HAMAP-Rule:MF_00058}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5,10-methylenetetrahydromethanopterin + 2 H(+) + oxidized
CC         coenzyme F420-(gamma-L-Glu)(n) = 5,10-methenyl-5,6,7,8-
CC         tetrahydromethanopterin + reduced coenzyme F420-(gamma-L-Glu)(n);
CC         Xref=Rhea:RHEA:16721, Rhea:RHEA-COMP:12939, Rhea:RHEA-COMP:14378,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57818, ChEBI:CHEBI:58337,
CC         ChEBI:CHEBI:133980, ChEBI:CHEBI:139511; EC=1.5.98.1;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00058};
CC   -!- SIMILARITY: Belongs to the MTD family. {ECO:0000255|HAMAP-
CC       Rule:MF_00058}.
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DR   EMBL; CP000102; ABC56581.1; -; Genomic_DNA.
DR   RefSeq; WP_011405780.1; NC_007681.1.
DR   AlphaFoldDB; Q2NHQ2; -.
DR   SMR; Q2NHQ2; -.
DR   STRING; 339860.Msp_0163; -.
DR   EnsemblBacteria; ABC56581; ABC56581; Msp_0163.
DR   GeneID; 41324736; -.
DR   KEGG; mst:Msp_0163; -.
DR   eggNOG; arCOG04382; Archaea.
DR   HOGENOM; CLU_1006890_0_0_2; -.
DR   OMA; KGCFMTK; -.
DR   OrthoDB; 46875at2157; -.
DR   Proteomes; UP000001931; Chromosome.
DR   GO; GO:0008901; F:ferredoxin hydrogenase activity; IEA:InterPro.
DR   GO; GO:0030268; F:methylenetetrahydromethanopterin dehydrogenase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0015948; P:methanogenesis; IEA:UniProtKB-UniRule.
DR   GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00058; MTD; 1.
DR   InterPro; IPR002844; MTD.
DR   InterPro; IPR036080; MTD_sf.
DR   Pfam; PF01993; MTD; 1.
DR   PIRSF; PIRSF005627; MTD; 1.
DR   SUPFAM; SSF102324; SSF102324; 1.
PE   3: Inferred from homology;
KW   One-carbon metabolism; Oxidoreductase; Reference proteome.
FT   CHAIN           1..276
FT                   /note="F420-dependent methylenetetrahydromethanopterin
FT                   dehydrogenase"
FT                   /id="PRO_1000117815"
SQ   SEQUENCE   276 AA;  30221 MW;  A144CF2B90D1EB5E CRC64;
     MVVKIGIVKV GNIGTSVLID MILDERADRE DIDVRTISSG AKMGKNQMDD VLPKIDEYNP
     DVIIFISPDP GAKSPSSARE TLSKKNIPTI VIGDGPGERA IGSMKEQGLG YIIVKADSMI
     GARREFLDAT EMAVFNSDVL MVLSTTGVFR LIHKTLDKVI RDIDEDKLYS LPELVITAEN
     AVDAADFKNP YAKSKAIAAY SIACQVSSMN VRACFKTKDY HVYIPLVSAA HEMMSAASKL
     AYEAREIEKS NDTVVRTAHR RNGSILYGTS LNDNKE