ID   MTD_METTH               Reviewed;         276 AA.
AC   Q50501;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   25-MAY-2022, entry version 119.
DE   RecName: Full=F420-dependent methylenetetrahydromethanopterin dehydrogenase;
DE            Short=MTD;
DE            EC=1.5.98.1;
DE   AltName: Full=Coenzyme F420-dependent N5,N10-methylenetetrahydromethanopterin dehydrogenase;
GN   Name=mtd; OrderedLocusNames=MTH_1464;
OS   Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM
OS   10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum).
OC   Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC   Methanobacteriales; Methanobacteriaceae; Methanothermobacter.
OX   NCBI_TaxID=187420;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX   PubMed=7730278; DOI=10.1128/jb.177.9.2460-2468.1995;
RA   Noelling J., Pihl T.D., Vriesema A., Reeve J.N.;
RT   "Organization and growth phase-dependent transcription of methane genes in
RT   two regions of the Methanobacterium thermoautotrophicum genome.";
RL   J. Bacteriol. 177:2460-2468(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX   PubMed=9371463; DOI=10.1128/jb.179.22.7135-7155.1997;
RA   Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J.,
RA   Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D.,
RA   Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R.,
RA   Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D.,
RA   Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A.,
RA   Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J.,
RA   Reeve J.N.;
RT   "Complete genome sequence of Methanobacterium thermoautotrophicum deltaH:
RT   functional analysis and comparative genomics.";
RL   J. Bacteriol. 179:7135-7155(1997).
CC   -!- FUNCTION: Catalyzes the reversible reduction of methenyl-H(4)MPT(+) to
CC       methylene-H(4)MPT. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5,10-methylenetetrahydromethanopterin + 2 H(+) + oxidized
CC         coenzyme F420-(gamma-L-Glu)(n) = 5,10-methenyl-5,6,7,8-
CC         tetrahydromethanopterin + reduced coenzyme F420-(gamma-L-Glu)(n);
CC         Xref=Rhea:RHEA:16721, Rhea:RHEA-COMP:12939, Rhea:RHEA-COMP:14378,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57818, ChEBI:CHEBI:58337,
CC         ChEBI:CHEBI:133980, ChEBI:CHEBI:139511; EC=1.5.98.1;
CC   -!- PATHWAY: One-carbon metabolism; methanogenesis from CO(2); 5,10-
CC       methylene-5,6,7,8-tetrahydromethanopterin from 5,10-methenyl-5,6,7,8-
CC       tetrahydromethanopterin (coenzyme F420 route): step 1/1.
CC   -!- SIMILARITY: Belongs to the MTD family. {ECO:0000305}.
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DR   EMBL; U19362; AAA87412.1; -; Genomic_DNA.
DR   EMBL; AE000666; AAB85939.1; -; Genomic_DNA.
DR   PIR; A69062; A69062.
DR   RefSeq; WP_010877074.1; NC_000916.1.
DR   AlphaFoldDB; Q50501; -.
DR   SMR; Q50501; -.
DR   STRING; 187420.MTH_1464; -.
DR   EnsemblBacteria; AAB85939; AAB85939; MTH_1464.
DR   GeneID; 24854574; -.
DR   KEGG; mth:MTH_1464; -.
DR   PATRIC; fig|187420.15.peg.1426; -.
DR   HOGENOM; CLU_1006890_0_0_2; -.
DR   OMA; KGCFMTK; -.
DR   UniPathway; UPA00640; UER00695.
DR   Proteomes; UP000005223; Chromosome.
DR   GO; GO:0008901; F:ferredoxin hydrogenase activity; IEA:InterPro.
DR   GO; GO:0030268; F:methylenetetrahydromethanopterin dehydrogenase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0019386; P:methanogenesis, from carbon dioxide; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00058; MTD; 1.
DR   InterPro; IPR002844; MTD.
DR   InterPro; IPR036080; MTD_sf.
DR   Pfam; PF01993; MTD; 1.
DR   PIRSF; PIRSF005627; MTD; 1.
DR   SUPFAM; SSF102324; SSF102324; 1.
PE   3: Inferred from homology;
KW   Methanogenesis; One-carbon metabolism; Oxidoreductase; Reference proteome.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250"
FT   CHAIN           2..276
FT                   /note="F420-dependent methylenetetrahydromethanopterin
FT                   dehydrogenase"
FT                   /id="PRO_0000075041"
FT   REGION          257..276
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   276 AA;  29601 MW;  18445E608FEE8457 CRC64;
     MVVKIGIIRC GNIGTSPVLD LLLDERADRP NIDVCVVGSG AKMNPDEIER AVPTMLEMDR
     DFVIFISPNP GAPGPAKARE LLSEADVPAM IIGDAPGLRV KDEIEEQGLG YIIVKADPMI
     GARREFLDPT EMASFNSDVI KVLAFTGAYR VVQNTIDAMI ADVEAGKAPE LPQVVIDTDV
     AVEAAGYENP YAKAKAMAAY EIATKVADID VKGCFMVQDP DKYIPIVASA HEMLAAAAKL
     AVEAREIEKA NDTVLRTPHG KEGETLSKTD LLAKPE