MTD_METTM
ID MTD_METTM Reviewed; 276 AA.
AC P55300; D9PYW5;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 25-MAY-2022, entry version 106.
DE RecName: Full=F420-dependent methylenetetrahydromethanopterin dehydrogenase;
DE Short=MTD {ECO:0000303|PubMed:7852356};
DE EC=1.5.98.1 {ECO:0000269|PubMed:1765081};
DE AltName: Full=Coenzyme F420-dependent N5,N10-methylenetetrahydromethanopterin dehydrogenase;
GN Name=mtd {ECO:0000303|PubMed:7852356}; OrderedLocusNames=MTBMA_c00500;
OS Methanothermobacter marburgensis (strain ATCC BAA-927 / DSM 2133 / JCM
OS 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium
OS thermoautotrophicum).
OC Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC Methanobacteriales; Methanobacteriaceae; Methanothermobacter.
OX NCBI_TaxID=79929;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-18, FUNCTION,
RP CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, PATHWAY, AND SUBUNIT.
RC STRAIN=ATCC BAA-927 / DSM 2133 / JCM 14651 / NBRC 100331 / OCM 82 /
RC Marburg;
RX PubMed=7852356; DOI=10.1074/jbc.270.6.2827;
RA Mukhopadhyay B., Purwantini E., Pihl T.D., Reeve J.N., Daniels L.;
RT "Cloning, sequencing, and transcriptional analysis of the coenzyme F420-
RT dependent methylene-5,6,7,8-tetrahydromethanopterin dehydrogenase gene from
RT Methanobacterium thermoautotrophicum strain Marburg and functional
RT expression in Escherichia coli.";
RL J. Biol. Chem. 270:2827-2832(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-927 / DSM 2133 / JCM 14651 / NBRC 100331 / OCM 82 /
RC Marburg;
RX PubMed=20802048; DOI=10.1128/jb.00844-10;
RA Liesegang H., Kaster A.K., Wiezer A., Goenrich M., Wollherr A., Seedorf H.,
RA Gottschalk G., Thauer R.K.;
RT "Complete genome sequence of Methanothermobacter marburgensis, a
RT methanoarchaeon model organism.";
RL J. Bacteriol. 192:5850-5851(2010).
RN [3]
RP FUNCTION, CATALYTIC ACTIVITY, ACTIVITY REGULATION, BIOPHYSICOCHEMICAL
RP PROPERTIES, AND PATHWAY.
RC STRAIN=ATCC BAA-927 / DSM 2133 / JCM 14651 / NBRC 100331 / OCM 82 /
RC Marburg;
RX PubMed=1765081; DOI=10.1111/j.1432-1033.1991.tb16491.x;
RA Von Buenau R., Zirngibl C., Thauer R.K., Klein A.;
RT "Hydrogen-forming and coenzyme-F420-reducing methylene
RT tetrahydromethanopterin dehydrogenase are genetically distinct enzymes in
RT Methanobacterium thermoautotrophicum (Marburg).";
RL Eur. J. Biochem. 202:1205-1208(1991).
CC -!- FUNCTION: Catalyzes the reversible reduction of methenyl-H(4)MPT(+) to
CC methylene-H(4)MPT. {ECO:0000269|PubMed:1765081,
CC ECO:0000269|PubMed:7852356}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=5,10-methylenetetrahydromethanopterin + 2 H(+) + oxidized
CC coenzyme F420-(gamma-L-Glu)(n) = 5,10-methenyl-5,6,7,8-
CC tetrahydromethanopterin + reduced coenzyme F420-(gamma-L-Glu)(n);
CC Xref=Rhea:RHEA:16721, Rhea:RHEA-COMP:12939, Rhea:RHEA-COMP:14378,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57818, ChEBI:CHEBI:58337,
CC ChEBI:CHEBI:133980, ChEBI:CHEBI:139511; EC=1.5.98.1;
CC Evidence={ECO:0000269|PubMed:1765081, ECO:0000269|PubMed:7852356};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16722;
CC Evidence={ECO:0000269|PubMed:1765081};
CC -!- ACTIVITY REGULATION: Activity requires salt; 100 mM sodium or potassium
CC salts of chloride, phosphate or sulfate are equally effective. Not
CC inactivated by O(2). Inhibited by hydrogen-producing 5,10-
CC methenyltetrahydromethanopterin hydrogenase which has a higher affinity
CC for their shared substrate (PubMed:1765081). Enzyme is O(2)-stable and
CC strictly dependent on coenzyme F420 (PubMed:7852356).
CC {ECO:0000269|PubMed:1765081, ECO:0000269|PubMed:7852356}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=70 uM for 5,10-methylenetetrahydromethanopterin
CC {ECO:0000269|PubMed:1765081};
CC Note=KM for coenzyme F420-(gamma-Glu)(n) is greater than 100 uM.
CC {ECO:0000269|PubMed:1765081};
CC pH dependence:
CC Optimum pH is 6.0 (PubMed:1765081). Optimum pH is 4.0
CC (PubMed:7852356). {ECO:0000269|PubMed:1765081,
CC ECO:0000269|PubMed:7852356};
CC Temperature dependence:
CC Optimum temperature is 55-65 degrees Celsius (PubMed:1765081,
CC PubMed:7852356). Thermostable up to 40 degrees Celsius
CC (PubMed:7852356). {ECO:0000269|PubMed:1765081,
CC ECO:0000269|PubMed:7852356};
CC -!- PATHWAY: One-carbon metabolism; methanogenesis from CO(2); 5,10-
CC methylene-5,6,7,8-tetrahydromethanopterin from 5,10-methenyl-5,6,7,8-
CC tetrahydromethanopterin (coenzyme F420 route): step 1/1.
CC {ECO:0000269|PubMed:1765081, ECO:0000269|PubMed:7852356}.
CC -!- SUBUNIT: Found to be tightly associated with methyl-coenzyme M
CC methylreductase. {ECO:0000305|PubMed:7852356}.
CC -!- INDUCTION: Enzyme activity is not detectable when grown anaerobically.
CC {ECO:0000269|PubMed:1765081}.
CC -!- SIMILARITY: Belongs to the MTD family. {ECO:0000305}.
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DR EMBL; L37108; AAC41463.1; -; Genomic_DNA.
DR EMBL; CP001710; ADL57660.1; -; Genomic_DNA.
DR RefSeq; WP_013294889.1; NC_014408.1.
DR AlphaFoldDB; P55300; -.
DR SMR; P55300; -.
DR STRING; 79929.MTBMA_c00500; -.
DR EnsemblBacteria; ADL57660; ADL57660; MTBMA_c00500.
DR GeneID; 9703755; -.
DR KEGG; mmg:MTBMA_c00500; -.
DR PATRIC; fig|79929.8.peg.48; -.
DR HOGENOM; CLU_1006890_0_0_2; -.
DR OMA; KGCFMTK; -.
DR OrthoDB; 46875at2157; -.
DR UniPathway; UPA00640; UER00695.
DR Proteomes; UP000000345; Chromosome.
DR GO; GO:0008901; F:ferredoxin hydrogenase activity; IEA:InterPro.
DR GO; GO:0030268; F:methylenetetrahydromethanopterin dehydrogenase activity; IDA:MENGO.
DR GO; GO:0019386; P:methanogenesis, from carbon dioxide; IEA:UniProtKB-UniPathway.
DR GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-UniRule.
DR HAMAP; MF_00058; MTD; 1.
DR InterPro; IPR002844; MTD.
DR InterPro; IPR036080; MTD_sf.
DR Pfam; PF01993; MTD; 1.
DR PIRSF; PIRSF005627; MTD; 1.
DR SUPFAM; SSF102324; SSF102324; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Methanogenesis; One-carbon metabolism;
KW Oxidoreductase.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:7852356"
FT CHAIN 2..276
FT /note="F420-dependent methylenetetrahydromethanopterin
FT dehydrogenase"
FT /id="PRO_0000075042"
FT REGION 253..276
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 276 AA; 29614 MW; E32142964BF3BAC3 CRC64;
MVVKIGIIKC GNIGTSPVLD LLLDERADRP NIDVCVVGSG AKMNPDEIER AVPTMLEMER
DFVIFISPNP GAPGPAKARE LLSAADVPAM IIGDAPGLRV KDEIEEQGLG YIIVKADPMI
GARREFLDPT EMASFNSDVI KVLAFTGAYR VVQNTIDAMI ADVEAGKAPE LPQVVIDTDK
AVEAAGYTNP YAKAKAMAAY EIATKVADID VRGCFMVQDP DQYIPIVASA HEMLSAAAKL
AIEAREIEKA NDTVLRTPHG KEGKTLSKKD LLAKPE
