MTEF4_ARATH
ID MTEF4_ARATH Reviewed; 541 AA.
AC Q9ZT96; Q93WJ2;
DT 11-MAY-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Transcription termination factor MTERF4, chloroplastic {ECO:0000305};
DE AltName: Full=Mitochondrial transcription termination factor 4 {ECO:0000303|PubMed:23087700};
DE AltName: Full=Protein BELAYA SMERT' {ECO:0000303|PubMed:21464319};
DE AltName: Full=Protein RUGOSA2 {ECO:0000303|PubMed:21790815};
DE Flags: Precursor;
GN Name=MTERF4 {ECO:0000303|PubMed:23087700};
GN Synonyms=BSM {ECO:0000303|PubMed:21464319},
GN RUG2 {ECO:0000303|PubMed:21790815}, TERF3 {ECO:0000303|PubMed:21464319};
GN OrderedLocusNames=At4g02990 {ECO:0000312|Araport:AT4G02990};
GN ORFNames=T4I9.13 {ECO:0000312|EMBL:AAC79107.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14993207; DOI=10.1101/gr.1515604;
RA Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA Weissenbach J., Salanoubat M.;
RT "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT combined approach to evaluate and improve Arabidopsis genome annotation.";
RL Genome Res. 14:406-413(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 296-541 AND 384-541.
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP FUNCTION, AND MUTAGENESIS OF PRO-420.
RX PubMed=21790815; DOI=10.1111/j.1365-313x.2011.04726.x;
RA Quesada V., Sarmiento-Manus R., Gonzalez-Bayon R., Hricova A.,
RA Perez-Marcos R., Gracia-Martinez E., Medina-Ruiz L., Leyva-Diaz E.,
RA Ponce M.R., Micol J.L.;
RT "Arabidopsis RUGOSA2 encodes an mTERF family member required for
RT mitochondrion, chloroplast and leaf development.";
RL Plant J. 68:738-753(2011).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=21464319; DOI=10.1073/pnas.1103442108;
RA Babiychuk E., Vandepoele K., Wissing J., Garcia-Diaz M., De Rycke R.,
RA Akbari H., Joubes J., Beeckman T., Jaensch L., Frentzen M.,
RA Van Montagu M.C., Kushnir S.;
RT "Plastid gene expression and plant development require a plastidic protein
RT of the mitochondrial transcription termination factor family.";
RL Proc. Natl. Acad. Sci. U.S.A. 108:6674-6679(2011).
RN [7]
RP GENE FAMILY.
RX PubMed=23087700; DOI=10.3389/fpls.2012.00233;
RA Kleine T.;
RT "Arabidopsis thaliana mTERF proteins: evolution and functional
RT classification.";
RL Front. Plant Sci. 3:233-233(2012).
CC -!- FUNCTION: Transcription termination factor required for processing and
CC steady-state levels of plastid transcripts. Required for splicing of
CC the chloroplastic Clp protease (ClpP) group IIa intron. Required for
CC maturation of 16S rRNA and 23S rRNA in the chloroplast. Essential for
CC embryogenesis (PubMed:21464319). Required for the maintenance of the
CC correct levels of transcripts in the mitochondria and chloroplasts.
CC {ECO:0000269|PubMed:21464319}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000269|PubMed:21464319, ECO:0000269|PubMed:21790815}.
CC Mitochondrion {ECO:0000269|PubMed:21790815}.
CC -!- DISRUPTION PHENOTYPE: Embryonic lethality when homozygous, due to
CC growth arrest at the late globular stage.
CC {ECO:0000269|PubMed:21464319}.
CC -!- MISCELLANEOUS: 'BELAYA SMERT' means white death in Russian.
CC {ECO:0000303|PubMed:21464319}.
CC -!- SIMILARITY: Belongs to the mTERF family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAK55704.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=BX827924; Type=Miscellaneous discrepancy; Note=Sequencing errors.; Evidence={ECO:0000305};
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DR EMBL; AF069442; AAC79107.1; -; Genomic_DNA.
DR EMBL; AL161495; CAB77784.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE82257.1; -; Genomic_DNA.
DR EMBL; CP002687; ANM67864.1; -; Genomic_DNA.
DR EMBL; BX827924; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AF378901; AAK55704.1; ALT_INIT; mRNA.
DR EMBL; AY052742; AAK96456.1; -; mRNA.
DR PIR; T01394; T01394.
DR RefSeq; NP_001329662.1; NM_001340410.1.
DR RefSeq; NP_192208.1; NM_116533.3.
DR AlphaFoldDB; Q9ZT96; -.
DR SMR; Q9ZT96; -.
DR IntAct; Q9ZT96; 2.
DR STRING; 3702.AT4G02990.1; -.
DR iPTMnet; Q9ZT96; -.
DR PaxDb; Q9ZT96; -.
DR PRIDE; Q9ZT96; -.
DR ProteomicsDB; 250809; -.
DR EnsemblPlants; AT4G02990.1; AT4G02990.1; AT4G02990.
DR EnsemblPlants; AT4G02990.2; AT4G02990.2; AT4G02990.
DR GeneID; 828116; -.
DR Gramene; AT4G02990.1; AT4G02990.1; AT4G02990.
DR Gramene; AT4G02990.2; AT4G02990.2; AT4G02990.
DR KEGG; ath:AT4G02990; -.
DR Araport; AT4G02990; -.
DR TAIR; locus:2139394; AT4G02990.
DR eggNOG; KOG1267; Eukaryota.
DR HOGENOM; CLU_024229_1_1_1; -.
DR InParanoid; Q9ZT96; -.
DR OMA; TIDMEEM; -.
DR OrthoDB; 523198at2759; -.
DR PhylomeDB; Q9ZT96; -.
DR PRO; PR:Q9ZT96; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q9ZT96; baseline and differential.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR GO; GO:0009536; C:plastid; IDA:TAIR.
DR GO; GO:0003690; F:double-stranded DNA binding; IEA:InterPro.
DR GO; GO:0003729; F:mRNA binding; IDA:TAIR.
DR GO; GO:0032502; P:developmental process; IMP:TAIR.
DR GO; GO:0006353; P:DNA-templated transcription, termination; IEA:UniProtKB-KW.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IMP:TAIR.
DR GO; GO:0042794; P:plastid rRNA transcription; IMP:TAIR.
DR GO; GO:0042793; P:plastid transcription; IMP:TAIR.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR GO; GO:0008380; P:RNA splicing; IMP:TAIR.
DR Gene3D; 1.25.70.10; -; 1.
DR InterPro; IPR003690; MTERF.
DR InterPro; IPR038538; MTERF_sf.
DR PANTHER; PTHR13068; PTHR13068; 1.
DR Pfam; PF02536; mTERF; 1.
DR SMART; SM00733; Mterf; 10.
PE 1: Evidence at protein level;
KW Chloroplast; Mitochondrion; Plastid; Reference proteome; Transcription;
KW Transcription regulation; Transcription termination; Transit peptide.
FT TRANSIT 1..45
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 46..541
FT /note="Transcription termination factor MTERF4,
FT chloroplastic"
FT /id="PRO_0000436197"
FT REGION 66..103
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 503..541
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 67..100
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 523..541
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 420
FT /note="P->L: In rug2-1; variegated leaves, reduced growth
FT and altered development of chloroplasts and mitochondria."
FT /evidence="ECO:0000269|PubMed:21790815"
SQ SEQUENCE 541 AA; 61549 MW; 44BF67C4BA3B8804 CRC64;
MKIRFCNGFT KPGFLLVHFE PPSFFAVRSR SLSDSTYGNL CNHKKRPGTG IGLTVQCAIA
NRRFSSRSLD SPRRERSSRS SSSSGRDRDR DKDKGRDSKS LYSRPSLLDM NKEKAANRAK
VYEFLRGIGI VPDELDGLEL PVTADVMKER VEFLHKLGLT IEDINNYPLV LGCSVKKNMV
PVLDYLGKLG VRKSTFTEFL RRYPQVLHSS VVIDLAPVVK YLQGLDIKPS DVPRVLERYP
EVLGFKLEGT MSTSVAYLVG IGVARREIGG ILTRYPEILG MRVARIIKPL VEYLEVLGIP
RLAAARLIEK RPHILGFELD DTVKPNVQIL QDFNVRETSL PSIIAQYPEI IGIDLKPKLD
TQRKLLCSAI HLNPEDLGSL IERMPQFVSL SESPMLKHID FLTKCGFSID QTREMVIGCP
QVLALNLGIM KLSFEYFQKE MKRPLQDLVD FPAFFTYGLE STVKPRHKKI IKKGIKCSLA
WMLNCSDEKF EQRMSYDTID IEEVETDPSS FDMNTLMQPE REEESDSEYE EEEDDDDEEF
A
