MTEF5_DROME
ID MTEF5_DROME Reviewed; 560 AA.
AC Q9VEB4;
DT 15-FEB-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Transcription termination factor 5, mitochondrial {ECO:0000305};
DE AltName: Full=Mitochondrial transcription termination factor 5 {ECO:0000303|PubMed:24068965};
DE Short=mTerf5 {ECO:0000303|PubMed:24068965};
DE Flags: Precursor;
GN Name=mTerf5 {ECO:0000303|PubMed:24068965, ECO:0000312|FlyBase:FBgn0038584};
GN ORFNames=CG7175 {ECO:0000312|FlyBase:FBgn0038584};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAL28649.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAL28649.1};
RC TISSUE=Embryo {ECO:0000312|EMBL:AAL28649.1};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4] {ECO:0000305}
RP FUNCTION, SUBUNIT, AND SUBCELLULAR LOCATION.
RX PubMed=22784680; DOI=10.1016/j.mito.2012.06.010;
RA Bruni F., Manzari C., Filice M., Loguercio Polosa P., Colella M.,
RA Carmone C., Hambardjieva E., Garcia-Diaz M., Cantatore P., Roberti M.;
RT "D-MTERF5 is a novel factor modulating transcription in Drosophila
RT mitochondria.";
RL Mitochondrion 12:492-499(2012).
RN [5] {ECO:0000305}
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=24068965; DOI=10.1371/journal.pgen.1003800;
RA Joers P., Lewis S.C., Fukuoh A., Parhiala M., Ellilae S., Holt I.J.,
RA Jacobs H.T.;
RT "Mitochondrial transcription terminator family members mTTF and mTerf5 have
RT opposing roles in coordination of mtDNA synthesis.";
RL PLoS Genet. 9:E1003800-E1003800(2013).
CC -!- FUNCTION: Binds promoter DNA and regulates initiation of transcription
CC (PubMed:22784680, PubMed:24068965). Regulates mitochondrial replication
CC and transcription (PubMed:22784680, PubMed:24068965). Required for
CC normal topology and maintenance of mitochondrial DNA (mtDNA) levels
CC (PubMed:22784680, PubMed:24068965). Regulates mtDNA replication by re-
CC activating replication after replication pausing (PubMed:24068965).
CC Likely to regulate replication pausing by coordinating with the
CC mitochondrial termination factor mTTF which promotes replication
CC pausing (PubMed:24068965). Their function in replication pausing
CC prevents unregulated replication that may occur for example by
CC collisions between the machineries of DNA replication and transcription
CC during mtDNA synthesis (PubMed:24068965). This ensures the
CC incorporation of RNA transcripts into replication intermediates at the
CC replication fork and allows for proper fork progression
CC (PubMed:24068965). Possibly functions downstream of Dref which
CC activates genes involved in mtDNA replication and maintenance
CC (PubMed:24068965). {ECO:0000269|PubMed:22784680,
CC ECO:0000269|PubMed:24068965}.
CC -!- SUBUNIT: Probably binds to the mTTF-DNA complex.
CC {ECO:0000269|PubMed:22784680}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:22784680}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown is lethal. Most mutants
CC fail to develop past the L3 larval stage, and the few pupal escapers do
CC not develop past the late pupal stages. {ECO:0000269|PubMed:24068965}.
CC -!- SIMILARITY: Belongs to the mTERF family. {ECO:0000305}.
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DR EMBL; AE014297; AAF55513.1; -; Genomic_DNA.
DR EMBL; AY061101; AAL28649.1; -; mRNA.
DR RefSeq; NP_650693.1; NM_142436.4.
DR AlphaFoldDB; Q9VEB4; -.
DR STRING; 7227.FBpp0083029; -.
DR PaxDb; Q9VEB4; -.
DR PRIDE; Q9VEB4; -.
DR DNASU; 42182; -.
DR EnsemblMetazoa; FBtr0083609; FBpp0083029; FBgn0038584.
DR GeneID; 42182; -.
DR KEGG; dme:Dmel_CG7175; -.
DR UCSC; CG7175-RA; d. melanogaster.
DR CTD; 42182; -.
DR FlyBase; FBgn0038584; mTerf5.
DR VEuPathDB; VectorBase:FBgn0038584; -.
DR eggNOG; ENOG502RXWB; Eukaryota.
DR HOGENOM; CLU_040703_0_0_1; -.
DR InParanoid; Q9VEB4; -.
DR OMA; RHPNWCH; -.
DR OrthoDB; 959676at2759; -.
DR PhylomeDB; Q9VEB4; -.
DR BioGRID-ORCS; 42182; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 42182; -.
DR PRO; PR:Q9VEB4; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0038584; Expressed in egg chamber and 15 other tissues.
DR GO; GO:0005759; C:mitochondrial matrix; IBA:GO_Central.
DR GO; GO:0005739; C:mitochondrion; IDA:FlyBase.
DR GO; GO:0003690; F:double-stranded DNA binding; IEA:InterPro.
DR GO; GO:0003676; F:nucleic acid binding; IBA:GO_Central.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR GO; GO:0006390; P:mitochondrial transcription; IMP:FlyBase.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR GO; GO:0006393; P:termination of mitochondrial transcription; IBA:GO_Central.
DR Gene3D; 1.25.70.10; -; 2.
DR InterPro; IPR003690; MTERF.
DR InterPro; IPR038538; MTERF_sf.
DR PANTHER; PTHR15437; PTHR15437; 1.
DR SMART; SM00733; Mterf; 4.
PE 1: Evidence at protein level;
KW DNA replication; Mitochondrion; Reference proteome; Transcription;
KW Transcription regulation; Transit peptide.
FT TRANSIT 1..23
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 24..560
FT /note="Transcription termination factor 5, mitochondrial"
FT /evidence="ECO:0000255"
FT /id="PRO_0000438964"
FT REGION 23..47
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 27..41
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 560 AA; 64830 MW; AAC96DC4C6750A9A CRC64;
MLRNGQNQAQ LLARSLGQLA RGMASSKRVS SKKEDLKPKL PKPPTVEIPM EEPLNASYLS
KTLGSSYRSW AAALEKHPEL KTLKRKDLLS SYDTLKSLDY SVDDIIAKPM IIYYGATTLA
NRHSVLQECG FHNVTVQTLA KYVTVVNKPI EVLKAHNYIP FDVKVAERLA GYFKDIKLPV
DLRELESETL TLKSLRQSLI NAYLRERLQM DDNDLQKLWR VYTRIRHKSF RAVQDTIELL
TKEFNFSAER LRKNSFLLYS EADNVRRILR EVPTIDSQDI REIGFRRPKI LMSTCDSLKQ
TLQHVHAFGI SEDAVLRCLE VLTLGPDTVL ERLRDLQEIE EFQVLGTNPR ILRLVHYQNK
ARLRLDYLNQ LRVRCASLHI LSCGSEAFAK FARDGSDRTK GRDIVVYLSN VLGKDVQVLR
NLLSRHPNWC HIPLLHVKQC LEYLRSKKFK LNEIFANIHL LLYPIKRIEE KMLLLQSPDA
QEDLQLPVAN FDSLSNNEIL TLILYLIESE FHFTGDGIWT EQHTHHVENF NNLLPDFPES
LNKVYKYGVK PAEKMIMERL
