MTF2_YEAST
ID MTF2_YEAST Reviewed; 440 AA.
AC P10849; D6VRV2; Q05430;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1989, sequence version 1.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=Mitochondrial transcription factor 2;
DE AltName: Full=Protein NAM1;
DE Flags: Precursor;
GN Name=MTF2; Synonyms=NAM1; OrderedLocusNames=YDL044C; ORFNames=D2705;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2183001; DOI=10.1007/bf00260480;
RA Lisowsky T.;
RT "Molecular analysis of the mitochondrial transcription factor mtf2 of
RT Saccharomyces cerevisiae.";
RL Mol. Gen. Genet. 220:186-190(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=D273-10B/GR3;
RX PubMed=2651895; DOI=10.1007/bf00427051;
RA Asher B.E., Groudinsky O., Dujardin G., Altamura N., Kermorgant M.,
RA Slonimski P.P.;
RT "Novel class of nuclear genes involved in both mRNA splicing and protein
RT synthesis in Saccharomyces cerevisiae mitochondria.";
RL Mol. Gen. Genet. 215:517-528(1989).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9046088;
RX DOI=10.1002/(sici)1097-0061(199701)13:1<65::aid-yea50>3.0.co;2-t;
RA Saren A.-M., Laamanen P., Lejarcegui J.B., Paulin L.;
RT "The sequence of a 36.7 kb segment on the left arm of chromosome IV from
RT Saccharomyces cerevisiae reveals 20 non-overlapping open reading frames
RT (ORFs) including SIT4, FAD1, NAM1, RNA11, SIR2, NAT1, PRP9, ACT2 and MPS1
RT and 11 new ORFs.";
RL Yeast 13:65-71(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [6]
RP POSSIBLE FUNCTION.
RX PubMed=8413192; DOI=10.1007/bf00280396;
RA Groudinsky O., Bousquet I., Wallis M.G., Slonimski P.P., Dujardin G.;
RT "The NAM1/MTF2 nuclear gene product is selectively required for the
RT stability and/or processing of mitochondrial transcripts of the atp6 and of
RT the mosaic, cox1 and cytb genes in Saccharomyces cerevisiae.";
RL Mol. Gen. Genet. 240:419-427(1993).
RN [7]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: Required for the processing and/or for the stability of the
CC CYTB and COX1 intron-containing pre-mRNAs and of the ATP6 transcript.
CC Could be a stem-loop RNA-binding protein that plays a role in
CC determining RNA stability.
CC -!- INTERACTION:
CC P10849; P42900: SLS1; NbExp=3; IntAct=EBI-11823, EBI-17364;
CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix.
CC -!- MISCELLANEOUS: Present with 2060 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; X51665; CAA35977.1; -; Genomic_DNA.
DR EMBL; X14719; CAA32845.1; -; Genomic_DNA.
DR EMBL; Z74092; CAA98603.1; -; Genomic_DNA.
DR EMBL; Z71781; CAA96445.1; ALT_SEQ; Genomic_DNA.
DR EMBL; BK006938; DAA11812.1; -; Genomic_DNA.
DR PIR; JV0015; TWBYM1.
DR RefSeq; NP_010240.1; NM_001180103.1.
DR AlphaFoldDB; P10849; -.
DR SMR; P10849; -.
DR BioGRID; 32015; 64.
DR DIP; DIP-5017N; -.
DR IntAct; P10849; 14.
DR MINT; P10849; -.
DR STRING; 4932.YDL044C; -.
DR MaxQB; P10849; -.
DR PaxDb; P10849; -.
DR PRIDE; P10849; -.
DR EnsemblFungi; YDL044C_mRNA; YDL044C; YDL044C.
DR GeneID; 851517; -.
DR KEGG; sce:YDL044C; -.
DR SGD; S000002202; MTF2.
DR VEuPathDB; FungiDB:YDL044C; -.
DR eggNOG; ENOG502RXV4; Eukaryota.
DR HOGENOM; CLU_056437_0_0_1; -.
DR InParanoid; P10849; -.
DR OMA; CNVDFYN; -.
DR BioCyc; YEAST:G3O-29464-MON; -.
DR PRO; PR:P10849; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; P10849; protein.
DR GO; GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; IDA:SGD.
DR GO; GO:0003723; F:RNA binding; IMP:SGD.
DR GO; GO:0032543; P:mitochondrial translation; IMP:SGD.
DR GO; GO:0006397; P:mRNA processing; IMP:SGD.
DR InterPro; IPR043837; Mtf2-like_C.
DR InterPro; IPR040009; Mtf2/C5D6.12-like.
DR PANTHER; PTHR39468; PTHR39468; 1.
DR Pfam; PF19189; Mtf2; 1.
PE 1: Evidence at protein level;
KW Mitochondrion; mRNA processing; Reference proteome; RNA-binding;
KW Transit peptide.
FT TRANSIT 1..15
FT /note="Mitochondrion"
FT CHAIN 16..440
FT /note="Mitochondrial transcription factor 2"
FT /id="PRO_0000021790"
SQ SEQUENCE 440 AA; 51192 MW; 8E2BA022DF80BBD7 CRC64;
MIRTSSILKN CNYRYIHCIH RCLLNEANLK DRKTHNVERV SNEKTFEQAL EEERKVFGEL
FEAGARVENM RHTNASKIID KYYNGLQDNS EGTSVKKEKI VFNHSQRAQR KLPNKDHEFL
KETAGNDYVY ERAEPSAIST KTISEQTRTL LEKIFDEDNS INKSNRELLN LNLRKGSGME
ALRQPVAHSN VKFSEEVMQE IGNKIRYQTT LDQVLEPHID YLREAVKSDY DLLRYLKQSL
DIYKKRNKDL ELKMNAESSN IFEDIRSACI NKPAELPKPL AMTLPYIIVK SLRLGDFDFP
ADRKYTLISY VYNECKNNMD ASLYLTICNV DFYNLLVQLL WENFQEIRYL RRVVTEMSVN
GVIGNIETVD ILDKIVKEMR SLNEDVFLEA GEQLSADEEV SSSANKIVNV GVLWNKDTNN
DLLIVENYLK SLKKNLTRDR
