ID   MTFA_ECOLI              Reviewed;         265 AA.
AC   P76346; Q2MAZ4;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-1998, sequence version 2.
DT   03-AUG-2022, entry version 134.
DE   RecName: Full=Protein MtfA;
DE   AltName: Full=Mlc titration factor A;
GN   Name=mtfA; Synonyms=yeeI; OrderedLocusNames=b1976, JW1958;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [3]
RP   FUNCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH MLC.
RC   STRAIN=K12;
RX   PubMed=16855233; DOI=10.1128/jb.00219-06;
RA   Becker A.-K., Zeppenfeld T., Staab A., Seitz S., Boos W., Morita T.,
RA   Aiba H., Mahr K., Titgemeyer F., Jahreis K.;
RT   "YeeI, a novel protein involved in modulation of the activity of the
RT   glucose-phosphotransferase system in Escherichia coli K-12.";
RL   J. Bacteriol. 188:5439-5449(2006).
CC   -!- FUNCTION: Involved in the regulation of ptsG expression by binding and
CC       inactivating Mlc. {ECO:0000269|PubMed:16855233}.
CC   -!- SUBUNIT: Interacts with Mlc with high affinity.
CC       {ECO:0000269|PubMed:16855233}.
CC   -!- INTERACTION:
CC       P76346; P50456: mlc; NbExp=2; IntAct=EBI-1126682, EBI-1116104;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16855233}.
CC   -!- SIMILARITY: Belongs to the MtfA family. {ECO:0000305}.
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DR   EMBL; U00096; AAC75041.2; -; Genomic_DNA.
DR   EMBL; AP009048; BAE76562.1; -; Genomic_DNA.
DR   RefSeq; NP_416484.2; NC_000913.3.
DR   RefSeq; WP_001302302.1; NZ_SSZK01000059.1.
DR   AlphaFoldDB; P76346; -.
DR   SMR; P76346; -.
DR   BioGRID; 4261319; 14.
DR   BioGRID; 850840; 5.
DR   IntAct; P76346; 19.
DR   STRING; 511145.b1976; -.
DR   MEROPS; M90.001; -.
DR   jPOST; P76346; -.
DR   PaxDb; P76346; -.
DR   PRIDE; P76346; -.
DR   EnsemblBacteria; AAC75041; AAC75041; b1976.
DR   EnsemblBacteria; BAE76562; BAE76562; BAE76562.
DR   GeneID; 946489; -.
DR   KEGG; ecj:JW1958; -.
DR   KEGG; eco:b1976; -.
DR   PATRIC; fig|1411691.4.peg.274; -.
DR   EchoBASE; EB3159; -.
DR   eggNOG; COG3228; Bacteria.
DR   HOGENOM; CLU_063037_2_0_6; -.
DR   OMA; EHSGEAW; -.
DR   PhylomeDB; P76346; -.
DR   BioCyc; EcoCyc:G7063-MON; -.
DR   BioCyc; MetaCyc:G7063-MON; -.
DR   PRO; PR:P76346; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0005829; C:cytosol; IDA:EcoCyc.
DR   GO; GO:0004177; F:aminopeptidase activity; IDA:EcoCyc.
DR   GO; GO:0008237; F:metallopeptidase activity; IEA:InterPro.
DR   GO; GO:0043433; P:negative regulation of DNA-binding transcription factor activity; IDA:EcoCyc.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProt.
DR   GO; GO:0051090; P:regulation of DNA-binding transcription factor activity; IBA:GO_Central.
DR   CDD; cd20169; Peptidase_M90_mtfA; 1.
DR   Gene3D; 1.10.472.150; -; 1.
DR   Gene3D; 3.40.390.10; -; 1.
DR   HAMAP; MF_01593; MtfA; 1.
DR   InterPro; IPR024079; MetalloPept_cat_dom_sf.
DR   InterPro; IPR010384; MtfA_fam.
DR   InterPro; IPR042252; MtfA_N.
DR   PANTHER; PTHR30164; PTHR30164; 1.
DR   Pfam; PF06167; Peptidase_M90; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Reference proteome.
FT   CHAIN           1..265
FT                   /note="Protein MtfA"
FT                   /id="PRO_0000169108"
SQ   SEQUENCE   265 AA;  30279 MW;  198E366587CCF8A5 CRC64;
     MIKWPWKVQE SAHQTALPWQ EALSIPLLTC LTEQEQSKLV TLAERFLQQK RLVPLQGFEL
     DSLRSCRIAL LFCLPVLELG LEWLDGFHEV LIYPAPFVVD DEWEDDIGLV HNQRIVQSGQ
     SWQQGPIVLN WLDIQDSFDA SGFNLIIHEV AHKLDTRNGD RASGVPFIPL REVAGWEHDL
     HAAMNNIQEE IELVGENAAS IDAYAASDPA ECFAVLSEYF FSAPELFAPR FPSLWQRFCQ
     FYQQDPLQRL HHANDTDSFS ATNVH