MTH12_DROME
ID MTH12_DROME Reviewed; 488 AA.
AC P83119; Q8INH4;
DT 02-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT 11-JUL-2003, sequence version 2.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Probable G-protein coupled receptor Mth-like 12;
DE AltName: Full=Protein methuselah-like 12;
DE Flags: Precursor;
GN Name=mthl12; Synonyms=Mth-like-12; ORFNames=CG32853;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP IDENTIFICATION.
RX PubMed=11274391; DOI=10.1073/pnas.051625298;
RA West A.P. Jr., Llamas L.L., Snow P.M., Benzer S., Bjorkman P.J.;
RT "Crystal structure of the ectodomain of Methuselah, a Drosophila G protein-
RT coupled receptor associated with extended lifespan.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:3744-3749(2001).
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 2 family. Mth
CC subfamily. {ECO:0000305}.
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DR EMBL; AE014297; AAN13556.2; -; Genomic_DNA.
DR RefSeq; NP_731791.2; NM_169508.2.
DR AlphaFoldDB; P83119; -.
DR STRING; 7227.FBpp0082214; -.
DR GlyGen; P83119; 5 sites.
DR PaxDb; P83119; -.
DR PRIDE; P83119; -.
DR EnsemblMetazoa; FBtr0082746; FBpp0082214; FBgn0045442.
DR GeneID; 261599; -.
DR KEGG; dme:Dmel_CG32853; -.
DR CTD; 261599; -.
DR FlyBase; FBgn0045442; mthl12.
DR VEuPathDB; VectorBase:FBgn0045442; -.
DR eggNOG; KOG4193; Eukaryota.
DR GeneTree; ENSGT00940000166745; -.
DR HOGENOM; CLU_002753_3_0_1; -.
DR InParanoid; P83119; -.
DR OMA; FMEYFIW; -.
DR OrthoDB; 1153592at2759; -.
DR PhylomeDB; P83119; -.
DR BioGRID-ORCS; 261599; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 261599; -.
DR PRO; PR:P83119; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Genevisible; P83119; DM.
DR GO; GO:0016021; C:integral component of membrane; ISS:FlyBase.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004930; F:G protein-coupled receptor activity; ISS:FlyBase.
DR GO; GO:0048856; P:anatomical structure development; IEA:UniProt.
DR GO; GO:0007166; P:cell surface receptor signaling pathway; IEA:InterPro.
DR GO; GO:0008340; P:determination of adult lifespan; ISS:UniProtKB.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; ISS:FlyBase.
DR GO; GO:0042594; P:response to starvation; ISS:UniProtKB.
DR Gene3D; 2.170.180.11; -; 1.
DR Gene3D; 2.30.160.11; -; 1.
DR InterPro; IPR022343; GCR1-cAMP_receptor.
DR InterPro; IPR017981; GPCR_2-like.
DR InterPro; IPR044860; Methusela_ecto_dom_1.
DR InterPro; IPR023311; Methusela_ecto_dom_2.
DR InterPro; IPR010596; Methuselah_N_dom.
DR InterPro; IPR036272; Methuselah_N_sf.
DR Pfam; PF06652; Methuselah_N; 1.
DR PRINTS; PR02001; GCR1CAMPR.
DR SUPFAM; SSF63877; SSF63877; 1.
DR PROSITE; PS50261; G_PROTEIN_RECEP_F2_4; 1.
PE 3: Inferred from homology;
KW Cell membrane; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW Membrane; Receptor; Reference proteome; Signal; Transducer; Transmembrane;
KW Transmembrane helix.
FT SIGNAL 1..17
FT /evidence="ECO:0000255"
FT CHAIN 18..488
FT /note="Probable G-protein coupled receptor Mth-like 12"
FT /id="PRO_0000013035"
FT TOPO_DOM 18..215
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 216..236
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 237..247
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 248..268
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 269..283
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 284..304
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 305..315
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 316..336
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 337..373
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 374..394
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 395..416
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 417..437
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 438..454
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 455..475
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 476..488
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 19
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 34
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 55
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 141
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 365
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 27..81
FT /evidence="ECO:0000250|UniProtKB:O97148"
FT DISULFID 83..88
FT /evidence="ECO:0000250|UniProtKB:O97148"
FT DISULFID 92..189
FT /evidence="ECO:0000250|UniProtKB:O97148"
FT DISULFID 93..104
FT /evidence="ECO:0000250|UniProtKB:O97148"
FT DISULFID 155..209
FT /evidence="ECO:0000250|UniProtKB:O97148"
SQ SEQUENCE 488 AA; 56781 MW; 3CE6B05F8D1DC103 CRC64;
MFLWLKCFCT LIIVTIAKNS SAKIPHCKYD ETINISHFKR LNDAYIYEHF EIPANLTGEF
DYKELMDGSK VPTEFPNLRG CICKVRPCIR ICCARKNILS NGECSDGVKN EIKLTMLDLT
MQDILLTDPT LAELNMIPQY NSTELLILRE QFQPCDEIVS LKRDEYTILK DGSILLHTSA
EILSNDQYCL YPEIYSDFPE TIRIINRRCY RNVMPGIAQL SVISVVGFIL TLAVYLSVEK
LRNLLGKCLI CSLFSMFMEY FIWTMDYFRL LQSICSAAGY MKYFFSMSSY LWFSVVSFHL
WELFTSLNRH EPQYRFLIYN TFVWCTAAIP TVVIFSMNQM WENDPGKSEW LPLVGYFGCS
VKDWNSSSWF YSHIPIVILN SFNVIMFVLT AIYIWKVKKG VKSFAQHDER NTTCLEFNVQ
TYIQFVRLFL IMGASWLLDQ LTRLAEDSHL LLDTIVLNLT VYLNAAFGIL IFVLLILKGS
TFKMIMER
