MTH14_DROME
ID MTH14_DROME Reviewed; 533 AA.
AC Q8SYV9;
DT 11-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Probable G-protein coupled receptor Mth-like 14;
DE AltName: Full=Protein methuselah-like 14;
DE Flags: Precursor;
GN Name=mthl14; ORFNames=CG32476;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|EMBL:AAL48909.1};
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000269|PubMed:10731132};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000305}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000269|PubMed:12537572};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000269|PubMed:12537569};
RC TISSUE=Embryo {ECO:0000269|PubMed:12537569};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 2 family. Mth
CC subfamily. {ECO:0000305}.
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DR EMBL; AY071287; AAL48909.1; -; mRNA.
DR EMBL; AE014296; AAN11432.1; -; Genomic_DNA.
DR RefSeq; NP_728509.1; NM_167821.3.
DR AlphaFoldDB; Q8SYV9; -.
DR STRING; 7227.FBpp0072402; -.
DR GlyGen; Q8SYV9; 8 sites.
DR PaxDb; Q8SYV9; -.
DR PRIDE; Q8SYV9; -.
DR DNASU; 318046; -.
DR EnsemblMetazoa; FBtr0072501; FBpp0072402; FBgn0052476.
DR GeneID; 318046; -.
DR KEGG; dme:Dmel_CG32476; -.
DR CTD; 318046; -.
DR FlyBase; FBgn0052476; mthl14.
DR VEuPathDB; VectorBase:FBgn0052476; -.
DR eggNOG; ENOG502TB76; Eukaryota.
DR GeneTree; ENSGT00940000162103; -.
DR HOGENOM; CLU_035859_0_0_1; -.
DR InParanoid; Q8SYV9; -.
DR OMA; DRGSHEI; -.
DR OrthoDB; 1288149at2759; -.
DR PhylomeDB; Q8SYV9; -.
DR BioGRID-ORCS; 318046; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 318046; -.
DR PRO; PR:Q8SYV9; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0052476; Expressed in Malpighian tubule and 20 other tissues.
DR Genevisible; Q8SYV9; DM.
DR GO; GO:0016021; C:integral component of membrane; ISS:FlyBase.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004930; F:G protein-coupled receptor activity; ISS:FlyBase.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; ISS:FlyBase.
DR Gene3D; 2.170.180.11; -; 1.
DR InterPro; IPR023311; Methusela_ecto_dom_2.
DR InterPro; IPR036272; Methuselah_N_sf.
DR SUPFAM; SSF63877; SSF63877; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW Membrane; Receptor; Reference proteome; Signal; Transducer; Transmembrane;
KW Transmembrane helix.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..533
FT /note="Probable G-protein coupled receptor Mth-like 14"
FT /id="PRO_0000013036"
FT TOPO_DOM 24..242
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 243..263
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 264..279
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 280..300
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 301..303
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 304..324
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 325..347
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 348..368
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 369..395
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 396..416
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 417..451
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 452..472
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 473..480
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 481..501
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 502..533
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 86..108
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 20
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 29
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 30
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 36
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 47
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 133
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 178
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 206
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 120..216
FT /evidence="ECO:0000250|UniProtKB:O97148"
SQ SEQUENCE 533 AA; 60134 MW; 6D13D70ECCBEAC49 CRC64;
MNLGHWNFLL ALISLQTFFN ASAQISTVNN SSKGSNNSNI FHEDTFNSTS IDVLDASIHS
TLVVPQTYST EAATISGARF ATSVPVQSPV DNPLDPADCS QREKYRKQPV ATPSSRLRKC
CPHGENLNIY RENQSDSMCD NGLLSFEPTI ISAVLFDNCI EDLEVETTLD YDIGNPCNSS
LLYDDKDDVF FVLQDGSLLI IDKFGNESYT VKEHYCLDID KSGHLFAFTC VTQVEEQIAF
AKVVFVAVLM LISMPCLLLV SYLHMTLRLL RNLHGLSLSL MSLCLASGYF VHSVVHIYGI
PNQGFIGYVI QFCILSYFFW YLCICFNVLL NVWYKLPCCI QCSKSWATFN FACYAVFAFS
GPATIVALTV QKGLPGMPSY FLQGLTESIR DSQRYFIPPV STILFLSFLL NIISFFGFQR
ISGYAKAEKN IQERKCLFDQ QKYEDVKKDA KCVSLLGIIM VVSWLLEIIT FYSGSNSNYL
ILCDMVNGLQ GVWVLLIFLV VRRRRTIILR WWYDRGSHEI EGTELQALSN SPT
