MTH3_DROME
ID MTH3_DROME Reviewed; 511 AA.
AC Q9V818;
DT 02-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT 02-AUG-2002, sequence version 3.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Probable G-protein coupled receptor Mth-like 3;
DE AltName: Full=Protein methuselah-like 3;
DE Flags: Precursor;
GN Name=mthl3; Synonyms=BEST:GM02553; ORFNames=CG6530;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP REVIEW.
RX PubMed=10908591; DOI=10.1083/jcb.150.2.f83;
RA Brody T., Cravchik A.;
RT "Drosophila melanogaster G protein-coupled receptors.";
RL J. Cell Biol. 150:F83-F88(2000).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-495 AND SER-500, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 2 family. Mth
CC subfamily. {ECO:0000305}.
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DR EMBL; AE013599; AAF57863.2; -; Genomic_DNA.
DR RefSeq; NP_001286526.1; NM_001299597.1.
DR RefSeq; NP_001286527.1; NM_001299598.1.
DR RefSeq; NP_663308.1; NM_145335.3.
DR AlphaFoldDB; Q9V818; -.
DR BioGRID; 62655; 2.
DR DIP; DIP-23972N; -.
DR IntAct; Q9V818; 3.
DR STRING; 7227.FBpp0086111; -.
DR GlyGen; Q9V818; 3 sites.
DR iPTMnet; Q9V818; -.
DR PaxDb; Q9V818; -.
DR DNASU; 36961; -.
DR EnsemblMetazoa; FBtr0086955; FBpp0086111; FBgn0028956.
DR EnsemblMetazoa; FBtr0340211; FBpp0309186; FBgn0028956.
DR EnsemblMetazoa; FBtr0340212; FBpp0309187; FBgn0028956.
DR GeneID; 36961; -.
DR KEGG; dme:Dmel_CG6530; -.
DR CTD; 36961; -.
DR FlyBase; FBgn0028956; mthl3.
DR VEuPathDB; VectorBase:FBgn0028956; -.
DR eggNOG; KOG4193; Eukaryota.
DR GeneTree; ENSGT00940000168274; -.
DR HOGENOM; CLU_002753_3_0_1; -.
DR InParanoid; Q9V818; -.
DR OMA; LSKNQAW; -.
DR OrthoDB; 1153592at2759; -.
DR PhylomeDB; Q9V818; -.
DR BioGRID-ORCS; 36961; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 36961; -.
DR PRO; PR:Q9V818; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0028956; Expressed in oviduct (Drosophila) and 23 other tissues.
DR ExpressionAtlas; Q9V818; baseline and differential.
DR Genevisible; Q9V818; DM.
DR GO; GO:0016021; C:integral component of membrane; ISS:FlyBase.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004930; F:G protein-coupled receptor activity; ISS:FlyBase.
DR GO; GO:0007166; P:cell surface receptor signaling pathway; IEA:InterPro.
DR GO; GO:0008340; P:determination of adult lifespan; ISS:UniProtKB.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; ISS:FlyBase.
DR GO; GO:0042594; P:response to starvation; ISS:UniProtKB.
DR CDD; cd00251; Mth_Ecto; 1.
DR Gene3D; 2.170.180.11; -; 1.
DR Gene3D; 2.30.160.11; -; 1.
DR InterPro; IPR017981; GPCR_2-like.
DR InterPro; IPR000832; GPCR_2_secretin-like.
DR InterPro; IPR044860; Methusela_ecto_dom_1.
DR InterPro; IPR023311; Methusela_ecto_dom_2.
DR InterPro; IPR010596; Methuselah_N_dom.
DR InterPro; IPR036272; Methuselah_N_sf.
DR Pfam; PF00002; 7tm_2; 1.
DR Pfam; PF06652; Methuselah_N; 1.
DR SUPFAM; SSF63877; SSF63877; 1.
DR PROSITE; PS50261; G_PROTEIN_RECEP_F2_4; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW Membrane; Phosphoprotein; Receptor; Reference proteome; Signal; Transducer;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..511
FT /note="Probable G-protein coupled receptor Mth-like 3"
FT /id="PRO_0000013025"
FT TOPO_DOM 22..215
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 216..236
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 237..245
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 246..266
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 267..273
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 274..294
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 295..317
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 318..338
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 339..366
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 367..387
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 388..422
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 423..443
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 444..452
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 453..473
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 474..511
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT MOD_RES 495
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 500
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT CARBOHYD 42
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 120
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 168
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000250|UniProtKB:O97148"
FT DISULFID 26..80
FT /evidence="ECO:0000250|UniProtKB:O97148"
FT DISULFID 82..87
FT /evidence="ECO:0000250|UniProtKB:O97148"
FT DISULFID 91..186
FT /evidence="ECO:0000250|UniProtKB:O97148"
FT DISULFID 92..103
FT /evidence="ECO:0000250|UniProtKB:O97148"
FT DISULFID 148..204
FT /evidence="ECO:0000250|UniProtKB:O97148"
SQ SEQUENCE 511 AA; 58757 MW; A525811B3188A0A0 CRC64;
MRIVIGSFTA FLLLLLQNSN AEIPGCDFFD TVDISKAPRF SNGSYLYEGL LIPAHLTAEY
DYKLLADDSK EKVASHVRGC ACHLRPCIRF CCPQYQKMQK SKCYGDMSED ELNKHDPFVN
VTLSDGSVVR RHFKEDLIVQ SDLAKPGCPR MYFLNHELPG NEFTLFENGS LLRHWDKVEL
SKREYCVQHL SFKDDSIRIA PHFCPLSSEH SRTWKTVAIV ISLICIILTI SVYLYVEKLR
NLHGKCFICY LASLFLGYFF LVLNVWKYSS GFCVTAGFLG YFSVIAAFFW LSVISLTLWN
SFSGNSSWLN RFLPQNRFLS YNLYAWGMAL LLTAITYIAD QVVKNEKLRP RVGVGKNCWI
YTGDMTVMIY FYGPMLLIIV FNITMFVLTA FRIMKVKKEA QNFTQQQKTT NRLNSDKQTY
ALFLRLFIIM GLSWSLEIIS FLLSKNQAWA KAFMVADYFN WSQGTVIFLL FVLRPSTLKL
LKERIKGGRD EAGASDEHIS LQNTKIDPSV F
