MTH4_DROME
ID MTH4_DROME Reviewed; 517 AA.
AC Q9V817; A1ZAT1;
DT 02-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT 18-APR-2012, sequence version 5.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=Probable G-protein coupled receptor Mth-like 4;
DE AltName: Full=Protein methuselah-like 4;
DE Flags: Precursor;
GN Name=mthl4; ORFNames=CG6536;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP REVIEW.
RX PubMed=10908591; DOI=10.1083/jcb.150.2.f83;
RA Brody T., Cravchik A.;
RT "Drosophila melanogaster G protein-coupled receptors.";
RL J. Cell Biol. 150:F83-F88(2000).
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 2 family. Mth
CC subfamily. {ECO:0000305}.
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DR EMBL; AE013599; AAO41361.1; -; Genomic_DNA.
DR RefSeq; NP_788394.1; NM_176214.3.
DR AlphaFoldDB; Q9V817; -.
DR SMR; Q9V817; -.
DR BioGRID; 62654; 1.
DR STRING; 7227.FBpp0086113; -.
DR GlyGen; Q9V817; 4 sites.
DR PaxDb; Q9V817; -.
DR PRIDE; Q9V817; -.
DR EnsemblMetazoa; FBtr0086957; FBpp0086113; FBgn0034219.
DR GeneID; 36960; -.
DR KEGG; dme:Dmel_CG6536; -.
DR UCSC; CG6536-RB; d. melanogaster.
DR CTD; 36960; -.
DR FlyBase; FBgn0034219; mthl4.
DR VEuPathDB; VectorBase:FBgn0034219; -.
DR eggNOG; KOG4193; Eukaryota.
DR GeneTree; ENSGT00940000168274; -.
DR HOGENOM; CLU_002753_3_0_1; -.
DR InParanoid; Q9V817; -.
DR OMA; THTACEG; -.
DR OrthoDB; 1153592at2759; -.
DR PhylomeDB; Q9V817; -.
DR BioGRID-ORCS; 36960; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 36960; -.
DR PRO; PR:Q9V817; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0034219; Expressed in oviduct (Drosophila) and 19 other tissues.
DR ExpressionAtlas; Q9V817; baseline and differential.
DR Genevisible; Q9V817; DM.
DR GO; GO:0016021; C:integral component of membrane; ISS:FlyBase.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004930; F:G protein-coupled receptor activity; ISS:FlyBase.
DR GO; GO:0007166; P:cell surface receptor signaling pathway; IEA:InterPro.
DR GO; GO:0008340; P:determination of adult lifespan; ISS:UniProtKB.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; ISS:FlyBase.
DR GO; GO:0042594; P:response to starvation; ISS:UniProtKB.
DR CDD; cd00251; Mth_Ecto; 1.
DR Gene3D; 2.170.180.11; -; 1.
DR Gene3D; 2.30.160.11; -; 1.
DR InterPro; IPR017981; GPCR_2-like.
DR InterPro; IPR000832; GPCR_2_secretin-like.
DR InterPro; IPR044860; Methusela_ecto_dom_1.
DR InterPro; IPR023311; Methusela_ecto_dom_2.
DR InterPro; IPR010596; Methuselah_N_dom.
DR InterPro; IPR036272; Methuselah_N_sf.
DR Pfam; PF00002; 7tm_2; 1.
DR Pfam; PF06652; Methuselah_N; 1.
DR SUPFAM; SSF63877; SSF63877; 1.
DR PROSITE; PS50261; G_PROTEIN_RECEP_F2_4; 1.
PE 3: Inferred from homology;
KW Cell membrane; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW Membrane; Receptor; Reference proteome; Signal; Transducer; Transmembrane;
KW Transmembrane helix.
FT SIGNAL 1..18
FT /evidence="ECO:0000255"
FT CHAIN 19..517
FT /note="Probable G-protein coupled receptor Mth-like 4"
FT /id="PRO_0000013026"
FT TOPO_DOM 19..212
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 213..233
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 234..242
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 243..263
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 264..272
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 273..293
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 294..319
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 320..340
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 341..363
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 364..384
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 385..414
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 415..435
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 436..459
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 460..480
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 481..517
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 39
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 117
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 165
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 456
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 23..77
FT /evidence="ECO:0000250|UniProtKB:O97148"
FT DISULFID 79..84
FT /evidence="ECO:0000250|UniProtKB:O97148"
FT DISULFID 88..183
FT /evidence="ECO:0000250|UniProtKB:O97148"
FT DISULFID 89..100
FT /evidence="ECO:0000250|UniProtKB:O97148"
FT DISULFID 145..201
FT /evidence="ECO:0000250|UniProtKB:O97148"
SQ SEQUENCE 517 AA; 59240 MW; 0AE6D94520534EF9 CRC64;
MRILLIAVLF LLMPKSNAEI PGCDFFDTVD ISKAPRFSNG SYLYEGLLIP AHLTAEYDYK
LLADDSKEKV ASHVRGCACH LRPCIRFCCP QYQKMQKSKC YGDMSEDELN KHDPFVNVTL
SDGSVVRRHF KEDLIVQSDL AKPGCPRMYF LNHELPGNEF TLFENGSLLR HWDKVELSKR
EYCVQHLSFK DDSIRIAPHF CPLSSEHSRT WKTVAIVISL ICIILTISVY LYVEKLRNLH
GKCFICYLAS LFLGYFFLVL NVWKYSSGFC VTAGFLGYFS VMAAFFWLSV IGIHLRIKFS
LASNCLHRLL PENPFRAYNL YAWGIPLIMT AITYTADQVV KNEKLRPRVG VGKNCWIYTG
DMTVMIYFYG PMLLLIAFNI IMFVLSAIYI YNIKKNVKGL VHKQQTNQQI NDQQMFAIFL
RLFILMGLSW SFEILSFLLT KQQAWARALM VADYFNWSQG TIIFVLFILK PSILKLIIAG
GRQNLPGSHH NSRSKAARYN STHTACEGSI ADPNAYC
