MTH8_DROME
ID MTH8_DROME Reviewed; 492 AA.
AC Q9W0V7; Q9W0V8;
DT 02-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 2.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Probable G-protein coupled receptor Mth-like 8;
DE AltName: Full=Protein methuselah-like 8;
DE Flags: Precursor;
GN Name=mthl8; ORFNames=CG32475;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Larva, and Pupae;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP REVIEW.
RX PubMed=10908591; DOI=10.1083/jcb.150.2.f83;
RA Brody T., Cravchik A.;
RT "Drosophila melanogaster G protein-coupled receptors.";
RL J. Cell Biol. 150:F83-F88(2000).
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 2 family. Mth
CC subfamily. {ECO:0000305}.
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DR EMBL; AE014296; AAF47322.2; -; Genomic_DNA.
DR EMBL; AY069723; AAL39868.1; -; mRNA.
DR RefSeq; NP_001261182.1; NM_001274253.1.
DR RefSeq; NP_611998.1; NM_138154.3.
DR AlphaFoldDB; Q9W0V7; -.
DR BioGRID; 63579; 3.
DR DIP; DIP-19668N; -.
DR STRING; 7227.FBpp0302930; -.
DR GlyGen; Q9W0V7; 8 sites.
DR PaxDb; Q9W0V7; -.
DR DNASU; 38013; -.
DR EnsemblMetazoa; FBtr0072462; FBpp0072364; FBgn0052475.
DR EnsemblMetazoa; FBtr0329896; FBpp0302930; FBgn0052475.
DR GeneID; 38013; -.
DR KEGG; dme:Dmel_CG32475; -.
DR CTD; 38013; -.
DR FlyBase; FBgn0052475; mthl8.
DR VEuPathDB; VectorBase:FBgn0052475; -.
DR eggNOG; ENOG502T9C6; Eukaryota.
DR GeneTree; ENSGT00940000170903; -.
DR HOGENOM; CLU_002753_3_0_1; -.
DR InParanoid; Q9W0V7; -.
DR OMA; HPCAFID; -.
DR OrthoDB; 1153592at2759; -.
DR PhylomeDB; Q9W0V7; -.
DR BioGRID-ORCS; 38013; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 38013; -.
DR PRO; PR:Q9W0V7; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0052475; Expressed in eye disc (Drosophila) and 18 other tissues.
DR ExpressionAtlas; Q9W0V7; baseline and differential.
DR Genevisible; Q9W0V7; DM.
DR GO; GO:0016021; C:integral component of membrane; ISS:FlyBase.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004930; F:G protein-coupled receptor activity; ISS:FlyBase.
DR GO; GO:0008340; P:determination of adult lifespan; ISS:UniProtKB.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; ISS:FlyBase.
DR GO; GO:0042594; P:response to starvation; ISS:UniProtKB.
DR CDD; cd00251; Mth_Ecto; 1.
DR Gene3D; 2.170.180.11; -; 1.
DR Gene3D; 2.30.160.11; -; 1.
DR InterPro; IPR044860; Methusela_ecto_dom_1.
DR InterPro; IPR023311; Methusela_ecto_dom_2.
DR InterPro; IPR010596; Methuselah_N_dom.
DR InterPro; IPR036272; Methuselah_N_sf.
DR Pfam; PF06652; Methuselah_N; 1.
DR SUPFAM; SSF63877; SSF63877; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW Membrane; Receptor; Reference proteome; Signal; Transducer; Transmembrane;
KW Transmembrane helix.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..492
FT /note="Probable G-protein coupled receptor Mth-like 8"
FT /id="PRO_0000013029"
FT TOPO_DOM 22..218
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 219..239
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 240..245
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 246..266
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 267..282
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 283..303
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 304..317
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 318..338
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 339..362
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 363..383
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 384..411
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 412..432
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 433..441
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 442..462
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 463..492
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 37
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 51
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 129
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 169
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 192
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 275
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 360
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 438
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 30..82
FT /evidence="ECO:0000250|UniProtKB:O97148"
FT DISULFID 84..89
FT /evidence="ECO:0000250|UniProtKB:O97148"
FT DISULFID 93..184
FT /evidence="ECO:0000250|UniProtKB:O97148"
FT DISULFID 94..107
FT /evidence="ECO:0000250|UniProtKB:O97148"
SQ SEQUENCE 492 AA; 56330 MW; 931F166A9C6AD1DE CRC64;
MAQFCILGVL LILSGTHCSW GFHEETHYPC AFIDTANITG SYGLDGPFVH NWTVIPRHFV
AVYDFVIENG IRIPASRHLR ACVCKTKPCV RICCLRGEIY DLEKRQCLVP VAGVSSLPSH
SHMEVELGNG SLRLVKLQPR FSIHVETPCE HMKAVTKGSE YVHWTLHENG TISHRGHIFS
KHYCFTPLLH GNSTWEWQPL ACAPEKLYFV LGVREWTYAI CLLIAILSMF IVLMVYLMCS
EMRNSFYGVA IKAYAICMIL GYALLAYLTL HNPANLSNAA CRILPSLALM NLVLSFYILS
FIAFKLYLSF YGVVFTKLMF WLIFTPIVLV AVGWSFFVGF SYYGSRLIFG GDTCWFDPRN
WSVMIYFYAP VFVACAISGF FYVLSQIYIR DQPDIETEKS FESIEKNRFK SFWKYFGYTA
VVWVVCICSF AFNYYWENRS HLNYAVSFCM AFHGFAALYA LIGKNQQIQN FLRRIDNGED
TCENSVPLSS FG
