MTH_DROSI
ID MTH_DROSI Reviewed; 515 AA.
AC P83120; B4QEZ6;
DT 02-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT 14-OCT-2008, sequence version 2.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=G-protein coupled receptor Mth;
DE AltName: Full=Protein methuselah;
DE Flags: Precursor;
GN Name=mth; ORFNames=GD25710;
OS Drosophila simulans (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7240;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS PHE-15; PRO-27; ALA-60;
RP ARG-277; LEU-345; ALA-442; VAL-491 AND ASN-496.
RC STRAIN=CT2.1S, CT5.1S, CT6.1S, DPF3.1S, GA2.1S, GA4.1S, GA5.1S, HFL1.1S,
RC HFL2.1S, HFL4.1S, VA6.1S, VA7.1S, and VA8.1S;
RX PubMed=10995474; DOI=10.1073/pnas.190338897;
RA Schmidt P.S., Duvernell D.D., Eanes W.F.;
RT "Adaptive evolution of a candidate gene for aging in Drosophila.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:10861-10865(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=17994087; DOI=10.1038/nature06341;
RG Drosophila 12 genomes consortium;
RT "Evolution of genes and genomes on the Drosophila phylogeny.";
RL Nature 450:203-218(2007).
CC -!- FUNCTION: Involved in biological aging and stress response. Essential
CC for adult survival (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 2 family. Mth
CC subfamily. {ECO:0000305}.
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DR EMBL; AF280590; AAK49857.1; -; Genomic_DNA.
DR EMBL; AF280591; AAK49858.1; -; Genomic_DNA.
DR EMBL; AF280592; AAK49859.1; -; Genomic_DNA.
DR EMBL; AF280593; AAK49860.1; -; Genomic_DNA.
DR EMBL; AF280594; AAK49861.1; -; Genomic_DNA.
DR EMBL; AF280595; AAK49862.1; -; Genomic_DNA.
DR EMBL; AF280596; AAK49863.1; -; Genomic_DNA.
DR EMBL; AF280597; AAK49864.1; -; Genomic_DNA.
DR EMBL; AF280598; AAK49865.1; -; Genomic_DNA.
DR EMBL; AF280599; AAK49866.1; -; Genomic_DNA.
DR EMBL; AF280600; AAK49867.1; -; Genomic_DNA.
DR EMBL; AF280601; AAK49868.1; -; Genomic_DNA.
DR EMBL; AF280602; AAK49869.1; -; Genomic_DNA.
DR EMBL; CM000362; EDX07023.1; -; Genomic_DNA.
DR RefSeq; XP_016029709.1; XM_016182032.1.
DR AlphaFoldDB; P83120; -.
DR SMR; P83120; -.
DR STRING; 7240.P83120; -.
DR EnsemblMetazoa; FBtr0225620; FBpp0224112; FBgn0043394.
DR GeneID; 6734418; -.
DR HOGENOM; CLU_002753_3_0_1; -.
DR OMA; DFCITAG; -.
DR PhylomeDB; P83120; -.
DR Proteomes; UP000000304; Chromosome 2r.
DR Bgee; FBgn0043394; Expressed in embryo and 3 other tissues.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IEA:UniProtKB-KW.
DR GO; GO:0007166; P:cell surface receptor signaling pathway; IEA:InterPro.
DR GO; GO:0008340; P:determination of adult lifespan; IMP:UniProtKB.
DR GO; GO:0042594; P:response to starvation; ISS:UniProtKB.
DR CDD; cd00251; Mth_Ecto; 1.
DR Gene3D; 2.170.180.11; -; 1.
DR Gene3D; 2.30.160.11; -; 1.
DR InterPro; IPR017981; GPCR_2-like.
DR InterPro; IPR000832; GPCR_2_secretin-like.
DR InterPro; IPR044860; Methusela_ecto_dom_1.
DR InterPro; IPR023311; Methusela_ecto_dom_2.
DR InterPro; IPR010596; Methuselah_N_dom.
DR InterPro; IPR036272; Methuselah_N_sf.
DR Pfam; PF00002; 7tm_2; 1.
DR Pfam; PF06652; Methuselah_N; 1.
DR SUPFAM; SSF63877; SSF63877; 1.
DR PROSITE; PS50261; G_PROTEIN_RECEP_F2_4; 1.
PE 3: Inferred from homology;
KW Cell membrane; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW Membrane; Receptor; Reference proteome; Signal; Transducer; Transmembrane;
KW Transmembrane helix.
FT SIGNAL 1..24
FT /evidence="ECO:0000250"
FT CHAIN 25..515
FT /note="G-protein coupled receptor Mth"
FT /id="PRO_0000013021"
FT TOPO_DOM 25..218
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 219..239
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 240..248
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 249..269
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 270..278
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 279..299
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 300..320
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 321..341
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 342..370
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 371..391
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 392..424
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 425..445
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 446..454
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 455..475
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 476..515
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 45
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000250"
FT CARBOHYD 109
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 123
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000250"
FT CARBOHYD 170
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000250"
FT CARBOHYD 198
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 449
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 29..83
FT /evidence="ECO:0000250|UniProtKB:O97148"
FT DISULFID 85..90
FT /evidence="ECO:0000250|UniProtKB:O97148"
FT DISULFID 94..188
FT /evidence="ECO:0000250|UniProtKB:O97148"
FT DISULFID 95..106
FT /evidence="ECO:0000250|UniProtKB:O97148"
FT DISULFID 150..209
FT /evidence="ECO:0000250|UniProtKB:O97148"
FT VARIANT 15
FT /note="V -> F (in strain: GA2.1S, HFL2.1S and HFL4.1S)"
FT /evidence="ECO:0000269|PubMed:10995474"
FT VARIANT 27
FT /note="L -> P (in strain: GA2.1S, HFL2.1S, HFL4.1S and
FT VA8.1S)"
FT /evidence="ECO:0000269|PubMed:10995474"
FT VARIANT 60
FT /note="T -> A (in strain: HFL2.1S, HFL4.1S and VA8.1S)"
FT /evidence="ECO:0000269|PubMed:10995474"
FT VARIANT 277
FT /note="K -> R (in strain: CT2.1S, CT5.1S, CT6.1S and
FT VA6.1S)"
FT /evidence="ECO:0000269|PubMed:10995474"
FT VARIANT 345
FT /note="V -> L (in strain: GA2.1S)"
FT /evidence="ECO:0000269|PubMed:10995474"
FT VARIANT 442
FT /note="G -> A (in strain: HFL2.1S, VA7.1S and VA8.1S)"
FT /evidence="ECO:0000269|PubMed:10995474"
FT VARIANT 491
FT /note="E -> V (in strain: HFL4.1S)"
FT /evidence="ECO:0000269|PubMed:10995474"
FT VARIANT 496
FT /note="D -> N (in strain: GA2.1S, GA4.1S, GA5.1S, HFL1.1S,
FT HFL2.1S, HFL4.1S, VA7.1S and VA8.1S)"
FT /evidence="ECO:0000269|PubMed:10995474"
SQ SEQUENCE 515 AA; 59601 MW; 234BD93FEF2BE32E CRC64;
MKTLFVLRIS TVILVVLVIQ KSYADILECD YFDTVDISAA QKLQNGSYLF EGLLVPAILT
GEYDFRILPD DSKQKVASHI RGCVCKLKPC VRFCCPHDHI MDNGVCNDNM SEEELTELDP
FLNVTLDDGS VSRRHFKNEL IVQWDLPMPC DGMFYLDNRE EQDQYTLFEN GTFFRHFDRV
TLSKREYCLQ HLTFADGNAT SIRIAPHNCL IVPSITGQTV VMITSLICMV LTIAVYLFVK
KLQNLHGKCF ICYMVCLFMG YLFLLFDLWQ ISISFCKPAG VLGYFFVMAA FLWLSVISLH
LWNTFRGSSH KASRFFSEHR FLAYNTYAWG MSMVLTGITV LADNVVEDQD WNPRVGEEGH
CWIYTQAWSA MLYFYGPMVF LIAFNITMFI LTARRIIGVK KDIQNFAHRQ ERKQKLNSDK
QTYTFFLRLF IIMGLSWSLE IGSYISQSNQ TWANVFLVAD YLNWSQGIII FILFILKRST
LRLLQESIRG EGEEVDNSEE EISLENTRFD RNVLS
