MTL5_HUMAN
ID MTL5_HUMAN Reviewed; 508 AA.
AC Q9Y4I5; A8K8J3; Q4G182; Q6P2E2; Q8NCC8;
DT 26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT 02-SEP-2008, sequence version 2.
DT 03-AUG-2022, entry version 154.
DE RecName: Full=Tesmin {ECO:0000303|PubMed:10191092};
DE AltName: Full=Metallothionein-like 5, testis-specific;
DE AltName: Full=Testis-specific metallothionein-like protein;
GN Name=TESMIN {ECO:0000312|HGNC:HGNC:7446}; Synonyms=MTL5;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3), AND VARIANT
RP ARG-223.
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Brain, and Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 79-508 (ISOFORM 1).
RC TISSUE=Testis;
RX PubMed=10191092; DOI=10.1006/geno.1999.5756;
RA Sugihara T., Wadhwa R., Kaul S.C., Mitsui Y.;
RT "A novel testis-specific metallothionein-like protein, tesmin, is an early
RT marker of male germ cell differentiation.";
RL Genomics 57:130-136(1999).
RN [5]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-40, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19608861; DOI=10.1126/science.1175371;
RA Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C.,
RA Olsen J.V., Mann M.;
RT "Lysine acetylation targets protein complexes and co-regulates major
RT cellular functions.";
RL Science 325:834-840(2009).
CC -!- FUNCTION: Essential for normal spermatogenesis and male fertility (By
CC similarity). Required for the completion of meiosis in male germ cells
CC (By similarity). {ECO:0000250|UniProtKB:Q9WTJ6}.
CC -!- INTERACTION:
CC Q9Y4I5-2; Q53EP0-3: FNDC3B; NbExp=3; IntAct=EBI-12840664, EBI-10242151;
CC Q9Y4I5-2; Q16656-4: NRF1; NbExp=3; IntAct=EBI-12840664, EBI-11742836;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9WTJ6}. Nucleus
CC {ECO:0000250|UniProtKB:Q9WTJ6}. Note=Predominantly localized to the
CC cytoplasm. Translocates from the cytoplasm to the nucleus in the G2/M
CC transition upon treatment with cadmium, cobalt or zinc.
CC {ECO:0000250|UniProtKB:Q9WTJ6}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q9Y4I5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9Y4I5-2; Sequence=VSP_035299;
CC Name=3;
CC IsoId=Q9Y4I5-3; Sequence=VSP_035297, VSP_035298;
CC -!- TISSUE SPECIFICITY: Expressed specifically in testis.
CC -!- SIMILARITY: Belongs to the lin-54 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD24668.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AK074817; BAC11228.1; -; mRNA.
DR EMBL; AK292358; BAF85047.1; -; mRNA.
DR EMBL; CH471076; EAW74719.1; -; Genomic_DNA.
DR EMBL; BC027593; AAH27593.1; -; mRNA.
DR EMBL; BC064579; AAH64579.1; -; mRNA.
DR EMBL; U86074; AAD24668.1; ALT_FRAME; mRNA.
DR CCDS; CCDS44661.1; -. [Q9Y4I5-2]
DR CCDS; CCDS8184.1; -. [Q9Y4I5-1]
DR RefSeq; NP_001034745.1; NM_001039656.1. [Q9Y4I5-2]
DR RefSeq; NP_004914.2; NM_004923.3. [Q9Y4I5-1]
DR AlphaFoldDB; Q9Y4I5; -.
DR SMR; Q9Y4I5; -.
DR BioGRID; 114992; 26.
DR IntAct; Q9Y4I5; 3.
DR STRING; 9606.ENSP00000255087; -.
DR DrugBank; DB09130; Copper.
DR DrugBank; DB12965; Silver.
DR GlyGen; Q9Y4I5; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q9Y4I5; -.
DR PhosphoSitePlus; Q9Y4I5; -.
DR BioMuta; TESMIN; -.
DR DMDM; 205371773; -.
DR EPD; Q9Y4I5; -.
DR jPOST; Q9Y4I5; -.
DR MassIVE; Q9Y4I5; -.
DR PaxDb; Q9Y4I5; -.
DR PeptideAtlas; Q9Y4I5; -.
DR PRIDE; Q9Y4I5; -.
DR ProteomicsDB; 86210; -. [Q9Y4I5-1]
DR ProteomicsDB; 86211; -. [Q9Y4I5-2]
DR ProteomicsDB; 86212; -. [Q9Y4I5-3]
DR Antibodypedia; 30600; 131 antibodies from 20 providers.
DR DNASU; 9633; -.
DR Ensembl; ENST00000255087.10; ENSP00000255087.5; ENSG00000132749.11. [Q9Y4I5-1]
DR Ensembl; ENST00000443940.6; ENSP00000403086.2; ENSG00000132749.11. [Q9Y4I5-3]
DR Ensembl; ENST00000544963.1; ENSP00000440968.1; ENSG00000132749.11. [Q9Y4I5-2]
DR GeneID; 9633; -.
DR KEGG; hsa:9633; -.
DR MANE-Select; ENST00000255087.10; ENSP00000255087.5; NM_004923.3; NP_004914.2.
DR UCSC; uc001ooc.4; human. [Q9Y4I5-1]
DR CTD; 9633; -.
DR DisGeNET; 9633; -.
DR GeneCards; TESMIN; -.
DR HGNC; HGNC:7446; TESMIN.
DR HPA; ENSG00000132749; Tissue enriched (testis).
DR MIM; 604374; gene.
DR neXtProt; NX_Q9Y4I5; -.
DR OpenTargets; ENSG00000132749; -.
DR PharmGKB; PA31249; -.
DR VEuPathDB; HostDB:ENSG00000132749; -.
DR eggNOG; KOG1171; Eukaryota.
DR GeneTree; ENSGT00940000161379; -.
DR HOGENOM; CLU_025199_0_0_1; -.
DR InParanoid; Q9Y4I5; -.
DR OMA; ILRIEFK; -.
DR OrthoDB; 389707at2759; -.
DR PhylomeDB; Q9Y4I5; -.
DR TreeFam; TF313189; -.
DR PathwayCommons; Q9Y4I5; -.
DR SignaLink; Q9Y4I5; -.
DR BioGRID-ORCS; 9633; 13 hits in 1083 CRISPR screens.
DR ChiTaRS; TESMIN; human.
DR GenomeRNAi; 9633; -.
DR Pharos; Q9Y4I5; Tbio.
DR PRO; PR:Q9Y4I5; -.
DR Proteomes; UP000005640; Chromosome 11.
DR RNAct; Q9Y4I5; protein.
DR Bgee; ENSG00000132749; Expressed in right testis and 105 other tissues.
DR ExpressionAtlas; Q9Y4I5; baseline and differential.
DR Genevisible; Q9Y4I5; HS.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0001673; C:male germ cell nucleus; IEA:Ensembl.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0046872; F:metal ion binding; NAS:UniProtKB.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0006875; P:cellular metal ion homeostasis; NAS:UniProtKB.
DR GO; GO:0007140; P:male meiotic nuclear division; ISS:UniProtKB.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR GO; GO:0010038; P:response to metal ion; NAS:UniProtKB.
DR GO; GO:0007283; P:spermatogenesis; ISS:UniProtKB.
DR InterPro; IPR005172; CRC.
DR InterPro; IPR028307; Lin-54_fam.
DR InterPro; IPR033467; Tesmin/TSO1-like_CXC.
DR PANTHER; PTHR12446; PTHR12446; 1.
DR Pfam; PF03638; TCR; 1.
DR SMART; SM01114; CXC; 2.
DR PROSITE; PS51634; CRC; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Cytoplasm; Developmental protein;
KW Differentiation; Meiosis; Nucleus; Reference proteome; Spermatogenesis.
FT CHAIN 1..508
FT /note="Tesmin"
FT /id="PRO_0000096626"
FT DOMAIN 292..405
FT /note="CRC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00971"
FT MOD_RES 40
FT /note="N6-acetyllysine"
FT /evidence="ECO:0007744|PubMed:19608861"
FT VAR_SEQ 251
FT /note="D -> A (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_035297"
FT VAR_SEQ 252..508
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_035298"
FT VAR_SEQ 307..508
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039,
FT ECO:0000303|PubMed:15489334"
FT /id="VSP_035299"
FT VARIANT 223
FT /note="C -> R (in dbSNP:rs12365708)"
FT /evidence="ECO:0000269|PubMed:14702039"
FT /id="VAR_054067"
FT VARIANT 260
FT /note="V -> A (in dbSNP:rs35773957)"
FT /id="VAR_062236"
FT CONFLICT 191
FT /note="S -> W (in Ref. 4; AAD24668)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 508 AA; 55025 MW; 44DA371A81E012C0 CRC64;
MEEGPLPGGL PSPEDAMVTE LLSPEGPFAS ENIGLKAPVK YEEDEFHVFK EAYLGPADPK
EPVLHAFNPA LGADCKGQVK AKLAGGDSDG GELLGEYPGI PELSALEDVA LLQAPQPPAC
NVHFLSSLLP AHRSPAVLPL GAWVLEGASH PGVRMIPVEI KEAGGTTTSN NPEEATLQNL
LAQESCCKFP SSQELEDASC CSLKKDSNPM VICQLKGGTQ MLCIDNSRTR ELKALHLVPQ
YQDQNNYLQS DVPKPMTALV GRFLPASTKL NLITQQLEGA LPSVVNGSAF PSGSTLPGPP
KITLAGYCDC FASGDFCNNC NCNNCCNNLH HDIERFKAIK ACLGRNPEAF QPKIGKGQLG
NVKPQHNKGC NCRRSGCLKN YCECYEAQIM CSSICKCIGC KNYEESPERK TLMSMPNYMQ
TGGLEGSHYL PPTKFSGLPR FSHDRRPSSC ISWEVVEATC ACLLAQGEEA EKEHCSKCLA
EQMILEEFGR CLSQILHTEF KSKGLKME
