MTL5_MOUSE
ID MTL5_MOUSE Reviewed; 475 AA.
AC Q9WTJ6; Q548K4; Q8VIE0; Q8VIE1;
DT 26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT 02-SEP-2008, sequence version 2.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Tesmin {ECO:0000303|PubMed:10191092};
DE AltName: Full=Metallothionein-like 5, testis-specific;
DE AltName: Full=Testis-specific metallothionein-like protein;
GN Name=Tesmin; Synonyms=Mtl5;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [MRNA] OF
RP 91-475 (ISOFORM 1), TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=ICR; TISSUE=Testis;
RX PubMed=10191092; DOI=10.1006/geno.1999.5756;
RA Sugihara T., Wadhwa R., Kaul S.C., Mitsui Y.;
RT "A novel testis-specific metallothionein-like protein, tesmin, is an early
RT marker of male germ cell differentiation.";
RL Genomics 57:130-136(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, AND
RP DEVELOPMENTAL STAGE.
RC STRAIN=BALB/cJ;
RX PubMed=11803038; DOI=10.1016/s0162-0134(01)00377-4;
RA Matsuura T., Kawasaki Y., Miwa K., Sutou S., Ohinata Y., Yoshida F.,
RA Mitsui Y.;
RT "Germ cell-specific nucleocytoplasmic shuttling protein, tesmin, responsive
RT to heavy metal stress in mouse testes.";
RL J. Inorg. Biochem. 88:183-191(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1), SUBCELLULAR LOCATION, AND
RP TISSUE SPECIFICITY.
RC STRAIN=129;
RX PubMed=12606435; DOI=10.1095/biolreprod.102.005603;
RA Sutou S., Miwa K., Matsuura T., Kawasaki Y., Ohinata Y., Mitsui Y.;
RT "Native tesmin is a 60-kilodalton protein that undergoes dynamic changes in
RT its localization during spermatogenesis in mice.";
RL Biol. Reprod. 68:1861-1869(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), AND TISSUE SPECIFICITY.
RC STRAIN=C57BL/6 X DBA/2; TISSUE=Testis;
RX PubMed=14648882; DOI=10.1002/mrd.20007;
RA Olesen C., Moeller M., Byskov A.G.;
RT "Tesmin transcription is regulated differently during male and female
RT meiosis.";
RL Mol. Reprod. Dev. 67:116-126(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-34, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [7]
RP FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION (ISOFORMS 1 AND 2),
RP AND TISSUE SPECIFICITY (ISOFORMS 1 AND 2).
RX PubMed=31916570; DOI=10.1093/biolre/ioaa002;
RA Oji A., Isotani A., Fujihara Y., Castaneda J.M., Oura S., Ikawa M.;
RT "Tesmin, Metallothionein-Like 5, is Required for Spermatogenesis in Mice.";
RL Biol. Reprod. 102:975-983(2020).
CC -!- FUNCTION: Essential for normal spermatogenesis and male fertility
CC (PubMed:31916570). Required for the completion of meiosis in male germ
CC cells (PubMed:31916570). {ECO:0000269|PubMed:31916570}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:11803038,
CC ECO:0000269|PubMed:12606435}. Nucleus {ECO:0000269|PubMed:11803038,
CC ECO:0000269|PubMed:12606435}. Note=Predominantly localized to the
CC cytoplasm (PubMed:11803038, PubMed:12606435). Translocates from the
CC cytoplasm to the nucleus in the G2/M transition upon treatment with
CC cadmium, cobalt or zinc (PubMed:11803038, PubMed:12606435).
CC {ECO:0000269|PubMed:11803038, ECO:0000269|PubMed:12606435}.
CC -!- SUBCELLULAR LOCATION: [Isoform 1]: Cytoplasm
CC {ECO:0000269|PubMed:31916570}.
CC -!- SUBCELLULAR LOCATION: [Isoform 2]: Nucleus
CC {ECO:0000269|PubMed:31916570}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=Long {ECO:0000303|PubMed:31916570}, Tesmin-L
CC {ECO:0000303|PubMed:31916570};
CC IsoId=Q9WTJ6-1; Sequence=Displayed;
CC Name=2; Synonyms=Short {ECO:0000303|PubMed:31916570}, Tesmin-S
CC {ECO:0000303|PubMed:31916570};
CC IsoId=Q9WTJ6-2; Sequence=VSP_035300;
CC -!- TISSUE SPECIFICITY: Expressed in spermatocytes and adult heart. Also
CC expressed in fetal brain, heart, kidney and ovary.
CC {ECO:0000269|PubMed:10191092, ECO:0000269|PubMed:12606435,
CC ECO:0000269|PubMed:14648882}.
CC -!- TISSUE SPECIFICITY: [Isoform 1]: Testis (at protein level).
CC {ECO:0000269|PubMed:31916570}.
CC -!- TISSUE SPECIFICITY: [Isoform 2]: Testis. {ECO:0000269|PubMed:31916570}.
CC -!- DEVELOPMENTAL STAGE: Expressed as early as day 8, coincident with the
CC entry of germ cells into meiosis. Expression then progressively
CC increases. Expressed in the pachytene stage.
CC {ECO:0000269|PubMed:10191092, ECO:0000269|PubMed:11803038}.
CC -!- DISRUPTION PHENOTYPE: Male mice are infertile due to arrested
CC spermatogenesis at the pachytene stage. {ECO:0000269|PubMed:31916570}.
CC -!- SIMILARITY: Belongs to the lin-54 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD24667.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; U67176; AAD24666.1; -; mRNA.
DR EMBL; U77383; AAD24667.1; ALT_INIT; mRNA.
DR EMBL; AB057423; BAB64935.1; -; mRNA.
DR EMBL; AB057422; BAB64934.1; -; Genomic_DNA.
DR EMBL; AF329359; AAM54491.1; -; mRNA.
DR EMBL; AF329360; AAM54492.1; -; mRNA.
DR EMBL; BC024377; AAH24377.1; -; mRNA.
DR CCDS; CCDS37880.1; -. [Q9WTJ6-1]
DR CCDS; CCDS70912.1; -. [Q9WTJ6-2]
DR RefSeq; NP_001034746.1; NM_001039657.2. [Q9WTJ6-1]
DR RefSeq; NP_001273486.1; NM_001286557.1. [Q9WTJ6-2]
DR RefSeq; NP_001273487.1; NM_001286558.1. [Q9WTJ6-2]
DR AlphaFoldDB; Q9WTJ6; -.
DR SMR; Q9WTJ6; -.
DR STRING; 10090.ENSMUSP00000025840; -.
DR iPTMnet; Q9WTJ6; -.
DR PhosphoSitePlus; Q9WTJ6; -.
DR PaxDb; Q9WTJ6; -.
DR PRIDE; Q9WTJ6; -.
DR ProteomicsDB; 291453; -. [Q9WTJ6-1]
DR ProteomicsDB; 291454; -. [Q9WTJ6-2]
DR Antibodypedia; 30600; 131 antibodies from 20 providers.
DR DNASU; 17771; -.
DR Ensembl; ENSMUST00000025840; ENSMUSP00000025840; ENSMUSG00000024905. [Q9WTJ6-1]
DR Ensembl; ENSMUST00000127142; ENSMUSP00000122687; ENSMUSG00000024905. [Q9WTJ6-2]
DR Ensembl; ENSMUST00000142193; ENSMUSP00000114171; ENSMUSG00000024905. [Q9WTJ6-2]
DR GeneID; 17771; -.
DR KEGG; mmu:17771; -.
DR UCSC; uc008fwg.2; mouse. [Q9WTJ6-1]
DR CTD; 9633; -.
DR MGI; MGI:1340029; Tesmin.
DR VEuPathDB; HostDB:ENSMUSG00000024905; -.
DR eggNOG; KOG1171; Eukaryota.
DR GeneTree; ENSGT00940000161379; -.
DR HOGENOM; CLU_025199_0_0_1; -.
DR InParanoid; Q9WTJ6; -.
DR OMA; ILRIEFK; -.
DR PhylomeDB; Q9WTJ6; -.
DR TreeFam; TF313189; -.
DR BioGRID-ORCS; 17771; 2 hits in 73 CRISPR screens.
DR PRO; PR:Q9WTJ6; -.
DR Proteomes; UP000000589; Chromosome 19.
DR RNAct; Q9WTJ6; protein.
DR Bgee; ENSMUSG00000024905; Expressed in spermatocyte and 25 other tissues.
DR ExpressionAtlas; Q9WTJ6; baseline and differential.
DR Genevisible; Q9WTJ6; MM.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0001673; C:male germ cell nucleus; IDA:MGI.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0007140; P:male meiotic nuclear division; IMP:UniProtKB.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR GO; GO:0007283; P:spermatogenesis; IMP:UniProtKB.
DR InterPro; IPR005172; CRC.
DR InterPro; IPR028307; Lin-54_fam.
DR InterPro; IPR033467; Tesmin/TSO1-like_CXC.
DR PANTHER; PTHR12446; PTHR12446; 1.
DR Pfam; PF03638; TCR; 1.
DR SMART; SM01114; CXC; 1.
DR PROSITE; PS51634; CRC; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Developmental protein; Differentiation;
KW Meiosis; Nucleus; Phosphoprotein; Reference proteome; Spermatogenesis.
FT CHAIN 1..475
FT /note="Tesmin"
FT /id="PRO_0000096627"
FT DOMAIN 263..372
FT /note="CRC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00971"
FT MOD_RES 34
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 67
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q5XHX9"
FT VAR_SEQ 1..180
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:10191092,
FT ECO:0000303|PubMed:14648882"
FT /id="VSP_035300"
FT CONFLICT 433
FT /note="Q -> H (in Ref. 3; BAB64934)"
FT /evidence="ECO:0000305"
FT CONFLICT 439
FT /note="Q -> H (in Ref. 3; BAB64934)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 475 AA; 50616 MW; 4373ED1188DCF79F CRC64;
MEDALLGAMT GPEDELGAEL FGSERVFADG LALSPAGGAA DRDELPVLAD AYLGATEPGE
PLLRALSPPP GAEVPAALLG DFPGLPELRS PDDAAPPPAY SVHVLSSLLP GARGPALLPL
SAGVRVIPVE IKEAGGSVPG GSPEDAAFQA PLAQESCCKF PSSQEAEEAS SCPRKKDSSP
MVICQLKGGA QMLCIDNCGA RELKALHLLP QYDDQSSFPQ SELPKPMTTL VGRLLPVPAK
LNLITQVDNG ALPSAVNGAA FPSGPALQGP PKITLSGYCD CFSSGDFCNS CSCNNLRHEL
ERFKAIKACL DRNPEAFQPK MGKGRLGAAK LRHSKGCNCK RSGCLKNYCE CYEAKIMCSS
ICKCIACKNY EESPERKMLM STPHYMEPGD FESSHYLSPA KFSGPPKLRK NRQAFSCISW
EVVEATCACL LAQGEEAEQE HCSPSLAEQM ILEEFGRCLS QILHIEFKSK GLKIE
