MTLR_BACSU
ID MTLR_BACSU Reviewed; 694 AA.
AC P96574; Q797N1;
DT 26-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1997, sequence version 1.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Transcriptional regulator MtlR;
DE AltName: Full=Mannitol operon transcriptional activator;
DE Short=Mtl operon transcriptional activator;
DE Includes:
DE RecName: Full=Putative phosphotransferase enzyme IIB component;
DE EC=2.7.1.197;
DE AltName: Full=Putative PTS system EIIB component;
DE Includes:
DE RecName: Full=Putative phosphotransferase enzyme IIA component;
DE AltName: Full=Putative PTS system EIIA component;
GN Name=mtlR; Synonyms=ydaA; OrderedLocusNames=BSU04160;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RA Kasahara Y., Nakai S., Lee S., Sadaie Y., Ogasawara N.;
RT "A 148 kbp sequence of the region between 35 and 47 degree of the Bacillus
RT subtilis genome.";
RL Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=168;
RX PubMed=12897001; DOI=10.1128/jb.185.16.4816-4824.2003;
RA Watanabe S., Hamano M., Kakeshita H., Bunai K., Tojo S., Yamaguchi H.,
RA Fujita Y., Wong S.-L., Yamane K.;
RT "Mannitol-1-phosphate dehydrogenase (MtlD) is required for mannitol and
RT glucitol assimilation in Bacillus subtilis: possible cooperation of mtl and
RT gut operons.";
RL J. Bacteriol. 185:4816-4824(2003).
CC -!- FUNCTION: Positively regulates the expression of the mtlAFD operon
CC involved in the uptake and catabolism of mannitol.
CC {ECO:0000269|PubMed:12897001}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=D-mannitol(out) + N(pros)-phospho-L-histidyl-[protein] = D-
CC mannitol 1-phosphate(in) + L-histidyl-[protein];
CC Xref=Rhea:RHEA:33363, Rhea:RHEA-COMP:9745, Rhea:RHEA-COMP:9746,
CC ChEBI:CHEBI:16899, ChEBI:CHEBI:29979, ChEBI:CHEBI:61381,
CC ChEBI:CHEBI:64837; EC=2.7.1.197;
CC -!- ACTIVITY REGULATION: The regulatory activity of MtlR is modulated by
CC phosphorylation and dephosphorylation of the various MtlR domains. It
CC becomes activated via phosphoryl group transfer from PEP, EI and HPr on
CC the two conserved histidine residues in the PRD 2 domain, whereas
CC phosphorylation of the EIIA-like domain on His-599 by the PTS EIIB-Mtl
CC domain of MtlA inactivates MtlR (By similarity). {ECO:0000250}.
CC -!- INDUCTION: Up-regulated by mannitol or glucitol.
CC {ECO:0000269|PubMed:12897001}.
CC -!- DISRUPTION PHENOTYPE: Cells lacking this gene cannot grow on either
CC mannitol or glucitol as sole carbon source.
CC {ECO:0000269|PubMed:12897001}.
CC -!- SIMILARITY: Belongs to the transcriptional antiterminator BglG family.
CC {ECO:0000305}.
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DR EMBL; AB001488; BAA19254.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB12223.1; -; Genomic_DNA.
DR PIR; A69768; A69768.
DR RefSeq; NP_388297.1; NC_000964.3.
DR RefSeq; WP_003246695.1; NZ_JNCM01000031.1.
DR AlphaFoldDB; P96574; -.
DR SMR; P96574; -.
DR STRING; 224308.BSU04160; -.
DR PaxDb; P96574; -.
DR PRIDE; P96574; -.
DR EnsemblBacteria; CAB12223; CAB12223; BSU_04160.
DR GeneID; 938253; -.
DR KEGG; bsu:BSU04160; -.
DR PATRIC; fig|224308.179.peg.442; -.
DR eggNOG; COG3711; Bacteria.
DR InParanoid; P96574; -.
DR OMA; YMTAREQ; -.
DR PhylomeDB; P96574; -.
DR BioCyc; BSUB:BSU04160-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0008982; F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity; IEA:InterPro.
DR GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:InterPro.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR Gene3D; 1.10.10.10; -; 2.
DR Gene3D; 3.40.930.10; -; 1.
DR InterPro; IPR013196; HTH_11.
DR InterPro; IPR007737; Mga_HTH.
DR InterPro; IPR011608; PRD.
DR InterPro; IPR036634; PRD_sf.
DR InterPro; IPR016152; PTrfase/Anion_transptr.
DR InterPro; IPR002178; PTS_EIIA_type-2_dom.
DR InterPro; IPR036095; PTS_EIIB-like_sf.
DR InterPro; IPR013011; PTS_EIIB_2.
DR InterPro; IPR003501; PTS_EIIB_2/3.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF08279; HTH_11; 1.
DR Pfam; PF05043; Mga; 1.
DR Pfam; PF00874; PRD; 2.
DR Pfam; PF00359; PTS_EIIA_2; 1.
DR Pfam; PF02302; PTS_IIB; 1.
DR SUPFAM; SSF46785; SSF46785; 2.
DR SUPFAM; SSF52794; SSF52794; 1.
DR SUPFAM; SSF55804; SSF55804; 1.
DR SUPFAM; SSF63520; SSF63520; 2.
DR PROSITE; PS51372; PRD_2; 2.
DR PROSITE; PS51094; PTS_EIIA_TYPE_2; 1.
DR PROSITE; PS51099; PTS_EIIB_TYPE_2; 1.
PE 2: Evidence at transcript level;
KW Activator; DNA-binding; Kinase; Phosphoprotein; Reference proteome; Repeat;
KW Transcription; Transcription regulation; Transferase.
FT CHAIN 1..694
FT /note="Transcriptional regulator MtlR"
FT /id="PRO_0000376081"
FT DOMAIN 195..300
FT /note="PRD 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00704"
FT DOMAIN 305..410
FT /note="PRD 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00704"
FT DOMAIN 413..502
FT /note="PTS EIIB type-2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00422"
FT DOMAIN 536..683
FT /note="PTS EIIA type-2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00417"
FT MOD_RES 230
FT /note="Phosphohistidine; by HPr"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00704"
FT MOD_RES 289
FT /note="Phosphohistidine; by HPr"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00704"
FT MOD_RES 342
FT /note="Phosphohistidine; by HPr"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00704"
FT MOD_RES 399
FT /note="Phosphohistidine; by HPr"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00704"
FT MOD_RES 419
FT /note="Phosphocysteine; by EIIA"
FT /evidence="ECO:0000255"
FT MOD_RES 599
FT /note="Phosphohistidine; by EIIB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00704"
SQ SEQUENCE 694 AA; 78853 MW; 540D906A19318343 CRC64;
MYMTAREQKL LKHLLLQNRY ITVTELAELM QVSTRTIHRE LKSIKPLMET VGLTLDKQPG
KGLKAVGSPE GKQKLLTDLS YEQHEYSADE RKLLILCSLL ESQEPVKLYT LAHDLQVTNA
TVSYDLDELE KWISPFGLTL IRKRGFGIQL IGPENAKRKI VGNLIVNRLD IQMFLEAVEL
NIKGKTDSSE KMFGVVSKGE LLKMERILFQ LKEKIAFSLS DSSYIALVVH LTYAIERIKL
GETITMEQNE LEELMNAKEY SSALEIAGEL ERAFGVTIPE AEVGYITIHL RSANRKYKTE
YKAQEIELET ALQTKRLIAF ISDKIRMDLT KNYSLYEGLI AHLEPAVSRI KENIEIYNPM
KEQIKRDYFL LYMAIEEGVE KYFPGMSFSD DEIAFIVLHF GSALEIKKEE AKVKALVVCS
SGIGSSKMLA SRLKKELPEI ESFDMSSLIE LKGKDVQAYD MIVSTVPIPY ENIDYIMVSP
LLNEEDANQV KQYIKRKIPL ILNKKRSSKE EAQQADVPDM LEAAESIGRY MEVIQDVLRH
FTLAQLKTNP DHSMLLLELF QQLKKDGLIR DPEKAAVCLA EREKQGGLGI PGTNMALYHL
KNDEIVLPFF KMFDLSTPYE VDGMDGNTLR MTRILVMMAP GSLSAEGSEI LSAISSAIIE
SGESMAGFQE EGGQELYQRL NRIFFTWMKE KNIL