MTLR_ECOLI
ID MTLR_ECOLI Reviewed; 195 AA.
AC P0AF10; P36563; Q2M7R4;
DT 20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 20-DEC-2005, sequence version 1.
DT 25-MAY-2022, entry version 102.
DE RecName: Full=Mannitol operon repressor {ECO:0000303|PubMed:8300537};
DE AltName: Full=Mannitol repressor protein {ECO:0000303|PubMed:8300537};
GN Name=mtlR {ECO:0000303|PubMed:8300537}; OrderedLocusNames=b3601, JW3575;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=K12;
RX PubMed=8300537; DOI=10.1128/jb.176.3.840-847.1994;
RA Figge R.M., Ramseier T.M., Saier M.H. Jr.;
RT "The mannitol repressor (MtlR) of Escherichia coli.";
RL J. Bacteriol. 176:840-847(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=1964486; DOI=10.1111/j.1365-2958.1990.tb02050.x;
RA Jaiang W., Wu L.F., Tomich J., Saier M.H. Jr., Nichaus W.G.;
RT "Corrected sequence of the mannitol (mtl) operon in Escherichia coli.";
RL Mol. Microbiol. 4:2003-2006(1990).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=8041620; DOI=10.1093/nar/22.13.2576;
RA Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.;
RT "Analysis of the Escherichia coli genome. V. DNA sequence of the region
RT from 76.0 to 81.5 minutes.";
RL Nucleic Acids Res. 22:2576-2586(1994).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [6]
RP FUNCTION, AND SUBUNIT.
RX PubMed=19840941; DOI=10.1074/jbc.m109.062679;
RA Tan K., Clancy S., Borovilos M., Zhou M., Hoerer S., Moy S., Volkart L.L.,
RA Sassoon J., Baumann U., Joachimiak A.;
RT "The mannitol operon repressor MtlR belongs to a new class of transcription
RT regulators in bacteria.";
RL J. Biol. Chem. 284:36670-36679(2009).
CC -!- FUNCTION: Involved in the repression of the expression of the mannitol
CC mtlADR operon (PubMed:8300537). Does not bind the operator/promoter
CC regulatory region of this operon (PubMed:19840941). Therefore, seems to
CC belong to a new class of transcription factors in bacteria that may
CC regulate gene expression indirectly, perhaps as a part of a larger
CC transcriptional complex. {ECO:0000269|PubMed:19840941,
CC ECO:0000269|PubMed:8300537}.
CC -!- SUBUNIT: Homodimer. Can also form higher level multimer aggregates.
CC {ECO:0000269|PubMed:19840941}.
CC -!- DISRUPTION PHENOTYPE: Inactivation of this gene leads to the
CC constitutive expression of the mannitol operon.
CC {ECO:0000269|PubMed:8300537}.
CC -!- SIMILARITY: Belongs to the MtlR/FumE family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U03845; AAA92662.1; -; Genomic_DNA.
DR EMBL; X51359; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; U00039; AAB18578.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76625.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77692.1; -; Genomic_DNA.
DR PIR; B36970; B36970.
DR RefSeq; NP_418058.1; NC_000913.3.
DR RefSeq; WP_000228269.1; NZ_SSZK01000022.1.
DR PDB; 6KCR; X-ray; 3.50 A; A/C=1-195.
DR PDBsum; 6KCR; -.
DR AlphaFoldDB; P0AF10; -.
DR SMR; P0AF10; -.
DR BioGRID; 4263545; 10.
DR BioGRID; 852423; 3.
DR IntAct; P0AF10; 11.
DR STRING; 511145.b3601; -.
DR PaxDb; P0AF10; -.
DR PRIDE; P0AF10; -.
DR EnsemblBacteria; AAC76625; AAC76625; b3601.
DR EnsemblBacteria; BAE77692; BAE77692; BAE77692.
DR GeneID; 58389551; -.
DR GeneID; 948116; -.
DR KEGG; ecj:JW3575; -.
DR KEGG; eco:b3601; -.
DR PATRIC; fig|1411691.4.peg.3106; -.
DR EchoBASE; EB1908; -.
DR eggNOG; COG3722; Bacteria.
DR HOGENOM; CLU_099448_0_0_6; -.
DR OMA; KMGMVQL; -.
DR PhylomeDB; P0AF10; -.
DR BioCyc; EcoCyc:PD00369; -.
DR PRO; PR:P0AF10; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IMP:EcoCyc.
DR InterPro; IPR007761; MtlR-like.
DR InterPro; IPR038026; MtlR-like_sf.
DR PANTHER; PTHR37941; PTHR37941; 1.
DR Pfam; PF05068; MtlR; 1.
DR SUPFAM; SSF158668; SSF158668; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..195
FT /note="Mannitol operon repressor"
FT /id="PRO_0000096628"
FT HELIX 24..37
FT /evidence="ECO:0007829|PDB:6KCR"
FT HELIX 42..64
FT /evidence="ECO:0007829|PDB:6KCR"
FT HELIX 73..80
FT /evidence="ECO:0007829|PDB:6KCR"
FT STRAND 84..86
FT /evidence="ECO:0007829|PDB:6KCR"
FT HELIX 89..99
FT /evidence="ECO:0007829|PDB:6KCR"
FT HELIX 104..121
FT /evidence="ECO:0007829|PDB:6KCR"
FT HELIX 132..139
FT /evidence="ECO:0007829|PDB:6KCR"
FT HELIX 158..187
FT /evidence="ECO:0007829|PDB:6KCR"
SQ SEQUENCE 195 AA; 21990 MW; 0BB49156ACF44354 CRC64;
MVDQAQDTLR PNNRLSDMQA TMEQTQAFEN RVLERLNAGK TVRSFLITAV ELLTEAVNLL
VLQVFRKDDY AVKYAVEPLL DGDGPLGDLS VRLKLIYGLG VINRQEYEDA ELLMALREEL
NHDGNEYAFT DDEILGPFGE LHCVAALPPP PQFEPADSSL YAMQIQRYQQ AVRSTMVLSL
TELISKISLK KAFQK
