ID   MTM4_METJA              Reviewed;         298 AA.
AC   Q58724;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 76.
DE   RecName: Full=Type II methyltransferase M.MjaIV {ECO:0000303|PubMed:12654995};
DE            Short=M.MjaIV {ECO:0000303|PubMed:12654995};
DE            EC=2.1.1.72;
GN   Name=mjaIVMP; OrderedLocusNames=MJ1328;
OS   Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS   10045 / NBRC 100440) (Methanococcus jannaschii).
OC   Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC   Methanocaldococcaceae; Methanocaldococcus.
OX   NCBI_TaxID=243232;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX   PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA   Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA   Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA   Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA   Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA   Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA   Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA   Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA   Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT   "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT   jannaschii.";
RL   Science 273:1058-1073(1996).
RN   [2]
RP   NOMENCLATURE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: A methylase that recognizes the double-stranded sequence 5'-
CC       GTNNAC-3', methylates A-5 on both strands, and protects the DNA from
CC       cleavage by the MjaIV endonuclease. {ECO:0000303|PubMed:12654995}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC         N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC         homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC         COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
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DR   EMBL; L77117; AAB99337.1; -; Genomic_DNA.
DR   PIR; G64465; G64465.
DR   AlphaFoldDB; Q58724; -.
DR   SMR; Q58724; -.
DR   STRING; 243232.MJ_1328; -.
DR   REBASE; 3895; M.MjaIV.
DR   EnsemblBacteria; AAB99337; AAB99337; MJ_1328.
DR   KEGG; mja:MJ_1328; -.
DR   eggNOG; arCOG03521; Archaea.
DR   HOGENOM; CLU_932594_0_0_2; -.
DR   PhylomeDB; Q58724; -.
DR   PRO; PR:Q58724; -.
DR   Proteomes; UP000000805; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:RHEA.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   Gene3D; 3.90.220.20; -; 1.
DR   InterPro; IPR044946; Restrct_endonuc_typeI_TRD_sf.
PE   4: Predicted;
KW   DNA-binding; Methyltransferase; Reference proteome; Restriction system;
KW   Transferase.
FT   CHAIN           1..298
FT                   /note="Type II methyltransferase M.MjaIV"
FT                   /id="PRO_0000088020"
SQ   SEQUENCE   298 AA;  35328 MW;  9BE52F35B001822B CRC64;
     MQKNQTIFLN TWKFLITLHQ KPWSTFFTKI PDFPHVLLKD IAKVGVGLVS GFDEAFLLNE
     DDISKLNEDE KQLIKNFVKA KNCKRFVVEG FVQYILIEDN LKDEEIFKTK YPNIYKKLLK
     FKDRMENRYL PKNKKWFNWQ ALRNYKFLIK NLNKKRIYVP TLDRKPYNRF SLGDDELLPS
     GDVIFIQPYN DDDIYFLLGY LNSSFFRNYY LANGGRRGGR VAFTQKLLEN AKIPTFSDEV
     KEKIKNIVKD IIYNLKNGKD IENLERQIDY IIVSAINNNQ FKGYQTTLKN LLKPKLKG