ID   MTMB2_METAC             Reviewed;         458 AA.
AC   P58866;
DT   20-JUN-2002, integrated into UniProtKB/Swiss-Prot.
DT   26-FEB-2008, sequence version 3.
DT   25-MAY-2022, entry version 88.
DE   RecName: Full=Monomethylamine methyltransferase MtmB2;
DE            Short=MMA methyltransferase 2;
DE            Short=MMAMT 2;
DE            EC=2.1.1.248;
GN   Name=mtmB2; OrderedLocusNames=MA_2972;
OS   Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 /
OS   C2A).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=188937;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35395 / DSM 2834 / JCM 12185 / C2A;
RX   PubMed=11932238; DOI=10.1101/gr.223902;
RA   Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W.,
RA   Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J.,
RA   Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P.,
RA   McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D.,
RA   Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R.,
RA   Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J.,
RA   Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A.,
RA   White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H.,
RA   Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V.,
RA   Zinder S.H., Lander E., Metcalf W.W., Birren B.;
RT   "The genome of Methanosarcina acetivorans reveals extensive metabolic and
RT   physiological diversity.";
RL   Genome Res. 12:532-542(2002).
CC   -!- FUNCTION: Catalyzes the transfer of the methyl group from
CC       monomethylamine to the corrinoid cofactor of MtmC. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Co(I)-[methylamine-specific corrinoid protein] + H(+) +
CC         methylamine = methyl-Co(III)-[methylamine-specific corrinoid protein]
CC         + NH4(+); Xref=Rhea:RHEA:26059, Rhea:RHEA-COMP:11120, Rhea:RHEA-
CC         COMP:11121, ChEBI:CHEBI:15378, ChEBI:CHEBI:28938, ChEBI:CHEBI:59338,
CC         ChEBI:CHEBI:85033, ChEBI:CHEBI:85035; EC=2.1.1.248;
CC   -!- PATHWAY: One-carbon metabolism; methanogenesis from methylamine.
CC   -!- SUBUNIT: Can form a complex with MtmC. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the monomethylamine methyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; AE010299; AAM06345.1; -; Genomic_DNA.
DR   STRING; 188937.MA_2972; -.
DR   KEGG; mac:MA_2972; -.
DR   HOGENOM; CLU_047925_0_0_2; -.
DR   OMA; MNMIAGW; -.
DR   UniPathway; UPA00643; -.
DR   Proteomes; UP000002487; Chromosome.
DR   GO; GO:0043852; F:monomethylamine methyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0015948; P:methanogenesis; IEA:UniProtKB-KW.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   Gene3D; 3.20.20.460; -; 1.
DR   InterPro; IPR008031; MtmB_MeTrfase.
DR   InterPro; IPR036655; MtmB_sf.
DR   Pfam; PF05369; MtmB; 1.
DR   SUPFAM; SSF75098; SSF75098; 1.
PE   3: Inferred from homology;
KW   Methanogenesis; Methyltransferase; Pyrrolysine; Reference proteome;
KW   Transferase.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250"
FT   CHAIN           2..458
FT                   /note="Monomethylamine methyltransferase MtmB2"
FT                   /id="PRO_0000216556"
FT   NON_STD         202
FT                   /note="Pyrrolysine"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   458 AA;  50344 MW;  CAB24BE4BA1D9D2B CRC64;
     MTFKKSFDCY DFYDRAKVGE KCTQDDWDLM KIPMKAMELK QKYGLDFKGE FVPTDRDMME
     KLFQAGFEML LECGIYCTDT HRIVKYTEDE IWDAINNVQK EFTLGTGRDA VNVRKRSVGD
     KRKPIVQGGP TGSPISEDVF MPVHMSYALE KEVDTIVNGV MTSVRGKPPV PKSPYEVLAA
     KTETRLIKQA CAMAGRPGMA VOGPETSLSA QGNISADCAG GMQSTDSHEV SQLNELKIDL
     DAIAVIAHYN ANSDIIMDEQ MPIFGGYAGG IEETTIVDVA THINAFVMSN ASWHLDGPVH
     IRWGSTNTRE TLTIAGWACA TISEFTDMLS GNQYYPCAGP GTEMCLLEAS AQSITDTASG
     REILSGVASA KGVVTDKTTG MEARMMGEVA RATAGAEITE INKILDKLVA LYEKNYASAP
     AGKTFQECYD VKTVTPTEEY MQIYDGARKK LEELGLVF