ID   MTMB2_METBA             Reviewed;         458 AA.
AC   Q9P9L4;
DT   20-JUN-2002, integrated into UniProtKB/Swiss-Prot.
DT   26-FEB-2008, sequence version 4.
DT   23-FEB-2022, entry version 52.
DE   RecName: Full=Monomethylamine methyltransferase MtmB2;
DE            Short=MMA methyltransferase 2;
DE            Short=MMAMT 2;
DE            EC=2.1.1.248;
GN   Name=mtmB2;
OS   Methanosarcina barkeri.
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=2208;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 43569 / MS / DSM 800 / JCM 10043 / NBRC 100474;
RA   Srinivasan G., Burke S.A., Lo S.L., Krzycki J.A.;
RT   "Expression of two nearly identical copies of genes for monomethylamine
RT   methyltransferase and their cognate corrinoid proteins in Methanosarcina
RT   barkeri.";
RL   Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the transfer of the methyl group from
CC       monomethylamine to the corrinoid cofactor of MtmC (MtmC1 or MtmC2).
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Co(I)-[methylamine-specific corrinoid protein] + H(+) +
CC         methylamine = methyl-Co(III)-[methylamine-specific corrinoid protein]
CC         + NH4(+); Xref=Rhea:RHEA:26059, Rhea:RHEA-COMP:11120, Rhea:RHEA-
CC         COMP:11121, ChEBI:CHEBI:15378, ChEBI:CHEBI:28938, ChEBI:CHEBI:59338,
CC         ChEBI:CHEBI:85033, ChEBI:CHEBI:85035; EC=2.1.1.248;
CC   -!- PATHWAY: One-carbon metabolism; methanogenesis from methylamine.
CC   -!- SUBUNIT: Can form a complex with MtmC (MtmC1 or MtmC2). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the monomethylamine methyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; AF230870; AAF68952.2; -; Genomic_DNA.
DR   PRIDE; Q9P9L4; -.
DR   BRENDA; 2.1.1.248; 3250.
DR   UniPathway; UPA00643; -.
DR   GO; GO:0043852; F:monomethylamine methyltransferase activity; IDA:MENGO.
DR   GO; GO:0015948; P:methanogenesis; IEA:UniProtKB-KW.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   Gene3D; 3.20.20.460; -; 1.
DR   InterPro; IPR008031; MtmB_MeTrfase.
DR   InterPro; IPR036655; MtmB_sf.
DR   Pfam; PF05369; MtmB; 1.
DR   SUPFAM; SSF75098; SSF75098; 1.
PE   3: Inferred from homology;
KW   Methanogenesis; Methyltransferase; Pyrrolysine; Transferase.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250"
FT   CHAIN           2..458
FT                   /note="Monomethylamine methyltransferase MtmB2"
FT                   /id="PRO_0000216551"
FT   NON_STD         202
FT                   /note="Pyrrolysine"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   458 AA;  50213 MW;  4E2370F091B7E5D2 CRC64;
     MTFRKYFDCY DFYDRAKVGE KCTLDDWDLM KIPMKAMELK QKYGLDFKGE FIPTDKDMME
     KLFKAGFEML LECGIYCTDT HRIVKYTEDE IWDAINNVQK EFVLGTGRDA VNVRKRSVGD
     KAKPIVQGGP TGSPISEDVF MPVHMSYALE KEVDTIVNGV MTTVRGKAPI PKSPYEVLAA
     KTETRLIKNA CAMAGRPGMG VOGPETSLSA QGNISADCAG GMTCTDSHEV SQLCELKIDL
     DAISVIAHYT ANSDIIMDEQ MPIFGGYAGG IEETTIVDVA THINAVLMSS ASWHLDGPVH
     IRWGSTNTRE TLMIAGWACA TISEFTDILS GNQYYPCAGP CTEMCLLEAS AQSITDTASG
     REILSGVASA KGVVTDKTTG MEARMMGEVA RATAGVEISE VNVILDKLVA LYEKNYASAP
     AGKTFQECYD VKTVTPTDEY MQVYDGARKK LEDLGLVF