ID   MTN5_NOSS7              Reviewed;         480 AA.
AC   P35678; K9QPL7;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   29-MAY-2013, sequence version 2.
DT   03-AUG-2022, entry version 73.
DE   RecName: Full=Type II methyltransferase M.NspV {ECO:0000303|PubMed:12654995};
DE            Short=M.NspV {ECO:0000303|PubMed:8367311};
DE            EC=2.1.1.-;
DE   AltName: Full=Methyltransferase NspV;
DE   AltName: Full=Modification methylase NspV;
GN   Name=nspVM {ECO:0000303|PubMed:8367311}; OrderedLocusNames=Nos7524_1224;
OS   Nostoc sp. (strain ATCC 29411 / PCC 7524).
OC   Bacteria; Cyanobacteria; Nostocales; Nostocaceae; Nostoc.
OX   NCBI_TaxID=28072;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 29411 / PCC 7524;
RX   PubMed=8367311; DOI=10.1093/nar/21.16.3899;
RA   Ueno T., Ito H., Kotani H., Nakajima K.;
RT   "Cloning and expression of the NspV restriction-modification genes of
RT   Nostoc sp. strain PCC7524.";
RL   Nucleic Acids Res. 21:3899-3899(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29411 / PCC 7524;
RX   PubMed=23277585; DOI=10.1073/pnas.1217107110;
RA   Shih P.M., Wu D., Latifi A., Axen S.D., Fewer D.P., Talla E., Calteau A.,
RA   Cai F., Tandeau de Marsac N., Rippka R., Herdman M., Sivonen K.,
RA   Coursin T., Laurent T., Goodwin L., Nolan M., Davenport K.W., Han C.S.,
RA   Rubin E.M., Eisen J.A., Woyke T., Gugger M., Kerfeld C.A.;
RT   "Improving the coverage of the cyanobacterial phylum using diversity-driven
RT   genome sequencing.";
RL   Proc. Natl. Acad. Sci. U.S.A. 110:1053-1058(2013).
RN   [3]
RP   NOMENCLATURE, AND SUBTYPE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: A gamma subtype methylase that recognizes the double-stranded
CC       sequence 5'-TTCGAA-3', and methylates it on an unknown base to protect
CC       it against the NspV endonuclease. {ECO:0000303|PubMed:12654995}.
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily.
CC       {ECO:0000305}.
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DR   EMBL; D14719; BAA03539.1; -; Genomic_DNA.
DR   EMBL; CP003552; AFY47111.1; -; Genomic_DNA.
DR   RefSeq; WP_015137567.1; NC_019684.1.
DR   AlphaFoldDB; P35678; -.
DR   STRING; 28072.Nos7524_1224; -.
DR   REBASE; 3475; M.NspV.
DR   REBASE; 57744; M.Nsp7524ORF1224P.
DR   PRIDE; P35678; -.
DR   EnsemblBacteria; AFY47111; AFY47111; Nos7524_1224.
DR   KEGG; nop:Nos7524_1224; -.
DR   PATRIC; fig|28072.8.peg.1339; -.
DR   eggNOG; COG0827; Bacteria.
DR   HOGENOM; CLU_044835_0_0_3; -.
DR   OMA; IIEPTCG; -.
DR   OrthoDB; 1178320at2; -.
DR   PRO; PR:P35678; -.
DR   Proteomes; UP000010378; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   SUPFAM; SSF53335; SSF53335; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Methyltransferase; Reference proteome; Restriction system;
KW   S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..480
FT                   /note="Type II methyltransferase M.NspV"
FT                   /id="PRO_0000087969"
FT   CONFLICT        314..315
FT                   /note="FP -> LG (in Ref. 1; BAA03539)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        409
FT                   /note="I -> V (in Ref. 1; BAA03539)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        423..480
FT                   /note="VYFLSFEDEEIAFKTVALLNSSLARDFYSSLVFWDEKRPIKSSILNSLDLEV
FT                   LAKVIL -> FTFSVLRMKKSPLKPLRF (in Ref. 1; BAA03539)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   480 AA;  55503 MW;  C56AFB0B52FB72EF CRC64;
     MIHNVEKTKL EYGDFQTPLE LAEKVCEKLV QLGVNPDIIV EPTCGLGNFI EAASYSFTRA
     KKIFGIEINS NYLDKIKEKQ IFLNDEKFDI RCGDFFQVDW SSIIGQLNKE ILIIGNLPWV
     TNSQQGSIDG ENLPIKNNFQ NYHGLDAITG KSNFDISEWM LIRLVQCLQK HNGYLAMLCK
     TSVSRKILNY IHSQKLNLAY SATYKIDTRK YFRVNVDACL FLCKFDLISK NYFCDIFDNI
     ETSKYYRIGY HNNVLIKDLV ALKRLKNLYT NKSNIKWRSG IKHDCASVME LQKINDTFIN
     GLGEIVDIEE TYLFPLIKGS YVAQNKINAT DRYILVTQKI IGEPTDNIRD LAPKTWQYLE
     KHEKLLDTRK SKIYQNKPRF SIFGVGSYSF SPWKIAICGL YKKLEFRLIG KISEKPTIFD
     DTVYFLSFED EEIAFKTVAL LNSSLARDFY SSLVFWDEKR PIKSSILNSL DLEVLAKVIL