ID   MTNA_HORVU              Reviewed;         367 AA.
AC   Q9AYT7;
DT   30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   25-MAY-2022, entry version 70.
DE   RecName: Full=Methylthioribose-1-phosphate isomerase {ECO:0000255|HAMAP-Rule:MF_03119};
DE            Short=M1Pi {ECO:0000255|HAMAP-Rule:MF_03119};
DE            Short=MTR-1-P isomerase {ECO:0000255|HAMAP-Rule:MF_03119};
DE            EC=5.3.1.23 {ECO:0000255|HAMAP-Rule:MF_03119};
DE   AltName: Full=Protein IRON DEFICIENCY INDUCIBLE 2;
DE            Short=HvIDI2;
DE   AltName: Full=S-methyl-5-thioribose-1-phosphate isomerase {ECO:0000255|HAMAP-Rule:MF_03119};
DE   AltName: Full=Translation initiation factor eIF-2B subunit alpha/beta/delta-like protein {ECO:0000255|HAMAP-Rule:MF_03119};
GN   Name=IDI2;
OS   Hordeum vulgare (Barley).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
OX   NCBI_TaxID=4513;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND INDUCTION.
RC   STRAIN=cv. Ehimehadaka No.1; TISSUE=Root;
RX   PubMed=11141174; DOI=10.1093/jexbot/51.353.2001;
RA   Yamaguchi H., Nakanishi H., Nishizawa N.K., Mori S.;
RT   "Isolation and characterization of IDI2, a new Fe-deficiency-induced cDNA
RT   from barley roots, which encodes a protein related to the alpha subunit of
RT   eukaryotic initiation factor 2B (eIF2Balpha).";
RL   J. Exp. Bot. 51:2001-2007(2000).
CC   -!- FUNCTION: Catalyzes the interconversion of methylthioribose-1-phosphate
CC       (MTR-1-P) into methylthioribulose-1-phosphate (MTRu-1-P).
CC       {ECO:0000255|HAMAP-Rule:MF_03119}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-methyl-5-thio-alpha-D-ribose 1-phosphate = S-methyl-5-thio-
CC         D-ribulose 1-phosphate; Xref=Rhea:RHEA:19989, ChEBI:CHEBI:58533,
CC         ChEBI:CHEBI:58548; EC=5.3.1.23; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_03119};
CC   -!- PATHWAY: Amino-acid biosynthesis; L-methionine biosynthesis via salvage
CC       pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate:
CC       step 1/6. {ECO:0000255|HAMAP-Rule:MF_03119}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03119}.
CC       Nucleus {ECO:0000255|HAMAP-Rule:MF_03119}.
CC   -!- INDUCTION: By iron deficiency in roots. {ECO:0000269|PubMed:11141174}.
CC   -!- SIMILARITY: Belongs to the eIF-2B alpha/beta/delta subunits family.
CC       MtnA subfamily. {ECO:0000255|HAMAP-Rule:MF_03119}.
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DR   EMBL; AB038775; BAB21393.1; -; mRNA.
DR   AlphaFoldDB; Q9AYT7; -.
DR   SMR; Q9AYT7; -.
DR   UniPathway; UPA00904; UER00874.
DR   ExpressionAtlas; Q9AYT7; baseline and differential.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0046523; F:S-methyl-5-thioribose-1-phosphate isomerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0019509; P:L-methionine salvage from methylthioadenosine; IEA:UniProtKB-UniPathway.
DR   GO; GO:0019284; P:L-methionine salvage from S-adenosylmethionine; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.20.120.420; -; 1.
DR   Gene3D; 3.40.50.10470; -; 1.
DR   HAMAP; MF_01678; Salvage_MtnA; 1.
DR   InterPro; IPR000649; IF-2B-related.
DR   InterPro; IPR005251; IF-M1Pi.
DR   InterPro; IPR042529; IF_2B-like_C.
DR   InterPro; IPR011559; Initiation_fac_2B_a/b/d.
DR   InterPro; IPR027363; M1Pi_N.
DR   InterPro; IPR037171; NagB/RpiA_transferase-like.
DR   Pfam; PF01008; IF-2B; 1.
DR   SUPFAM; SSF100950; SSF100950; 1.
DR   TIGRFAMs; TIGR00524; eIF-2B_rel; 1.
DR   TIGRFAMs; TIGR00512; salvage_mtnA; 1.
PE   2: Evidence at transcript level;
KW   Amino-acid biosynthesis; Cytoplasm; Isomerase; Methionine biosynthesis;
KW   Nucleus.
FT   CHAIN           1..367
FT                   /note="Methylthioribose-1-phosphate isomerase"
FT                   /id="PRO_0000401990"
FT   ACT_SITE        250
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03119"
FT   SITE            170
FT                   /note="Transition state stabilizer"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03119"
SQ   SEQUENCE   367 AA;  38575 MW;  7A28005A3273B8D2 CRC64;
     MGESSALQSI LYGRGALRLL DQRKLPLEEV YVDVKDSADG WNAIRDMVVR GAPAIAIAAA
     LSLAVEVNDH NFIGTPAEAA SFVSKKLEYL VSSRPTAVNL SDAATKLQNL VSTTAETAKD
     AKSIFQVFIE AAETMLVDDV ADNKAIGLHG AEFLQRQLGS SKNISVLTHC NTGSLATAGY
     GTALGVIRAL HSGGVLEKAF CTETRPFNQG SRLTAFELVH DKIPATLIAD SAAAALMNNG
     QVQAVIVGAD RIAANGDTAN KIGTYNLSIA AKHHGVQFYV AAPVTSIDLS LPSGKQIVIE
     ERSPKELLNS EGGLGKQVAA SGISVWNPAF DVTPADLITA IITEKGVITK SDPDGTFDIK
     SFIECAK