MTNA_TOBAC
ID MTNA_TOBAC Reviewed; 374 AA.
AC Q948X8;
DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 25-MAY-2022, entry version 66.
DE RecName: Full=Methylthioribose-1-phosphate isomerase {ECO:0000255|HAMAP-Rule:MF_03119};
DE Short=M1Pi {ECO:0000255|HAMAP-Rule:MF_03119};
DE Short=MTR-1-P isomerase {ECO:0000255|HAMAP-Rule:MF_03119};
DE EC=5.3.1.23 {ECO:0000255|HAMAP-Rule:MF_03119};
DE AltName: Full=S-methyl-5-thioribose-1-phosphate isomerase {ECO:0000255|HAMAP-Rule:MF_03119};
DE AltName: Full=Translation initiation factor eIF-2B subunit alpha/beta/delta-like protein {ECO:0000255|HAMAP-Rule:MF_03119};
GN Name=CIG2;
OS Nicotiana tabacum (Common tobacco).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC Nicotiana.
OX NCBI_TaxID=4097;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND INDUCTION.
RX PubMed=11471724; DOI=10.1271/bbb.65.1275;
RA Kimura T., Nakano T., Taki N., Ishikawa M., Asami T., Yoshida S.;
RT "Cytokinin-induced gene expression in cultured green cells of Nicotiana
RT tabacum identified by fluorescent differential display.";
RL Biosci. Biotechnol. Biochem. 65:1275-1283(2001).
CC -!- FUNCTION: Catalyzes the interconversion of methylthioribose-1-phosphate
CC (MTR-1-P) into methylthioribulose-1-phosphate (MTRu-1-P).
CC {ECO:0000255|HAMAP-Rule:MF_03119}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-methyl-5-thio-alpha-D-ribose 1-phosphate = S-methyl-5-thio-
CC D-ribulose 1-phosphate; Xref=Rhea:RHEA:19989, ChEBI:CHEBI:58533,
CC ChEBI:CHEBI:58548; EC=5.3.1.23; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_03119};
CC -!- PATHWAY: Amino-acid biosynthesis; L-methionine biosynthesis via salvage
CC pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate:
CC step 1/6. {ECO:0000255|HAMAP-Rule:MF_03119}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03119}.
CC Nucleus {ECO:0000255|HAMAP-Rule:MF_03119}.
CC -!- INDUCTION: By cytokinin (kinetin) in suspension cell culture.
CC {ECO:0000269|PubMed:11471724}.
CC -!- SIMILARITY: Belongs to the eIF-2B alpha/beta/delta subunits family.
CC MtnA subfamily. {ECO:0000255|HAMAP-Rule:MF_03119}.
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DR EMBL; AB031321; BAB63262.1; -; mRNA.
DR RefSeq; NP_001312120.1; NM_001325191.1.
DR AlphaFoldDB; Q948X8; -.
DR SMR; Q948X8; -.
DR STRING; 4097.Q948X8; -.
DR GeneID; 107774973; -.
DR KEGG; nta:107774973; -.
DR OMA; RLWVDET; -.
DR PhylomeDB; Q948X8; -.
DR UniPathway; UPA00904; UER00874.
DR Proteomes; UP000084051; Unplaced.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0046523; F:S-methyl-5-thioribose-1-phosphate isomerase activity; IBA:GO_Central.
DR GO; GO:0019509; P:L-methionine salvage from methylthioadenosine; IBA:GO_Central.
DR GO; GO:0019284; P:L-methionine salvage from S-adenosylmethionine; IEA:UniProtKB-UniRule.
DR Gene3D; 1.20.120.420; -; 1.
DR Gene3D; 3.40.50.10470; -; 1.
DR HAMAP; MF_01678; Salvage_MtnA; 1.
DR InterPro; IPR000649; IF-2B-related.
DR InterPro; IPR005251; IF-M1Pi.
DR InterPro; IPR042529; IF_2B-like_C.
DR InterPro; IPR011559; Initiation_fac_2B_a/b/d.
DR InterPro; IPR027363; M1Pi_N.
DR InterPro; IPR037171; NagB/RpiA_transferase-like.
DR Pfam; PF01008; IF-2B; 1.
DR SUPFAM; SSF100950; SSF100950; 1.
DR TIGRFAMs; TIGR00524; eIF-2B_rel; 1.
DR TIGRFAMs; TIGR00512; salvage_mtnA; 1.
PE 2: Evidence at transcript level;
KW Amino-acid biosynthesis; Cytoplasm; Isomerase; Methionine biosynthesis;
KW Nucleus; Reference proteome.
FT CHAIN 1..374
FT /note="Methylthioribose-1-phosphate isomerase"
FT /id="PRO_0000401991"
FT ACT_SITE 255
FT /note="Proton donor"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03119"
FT SITE 175
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03119"
SQ SEQUENCE 374 AA; 39570 MW; 155DD34ACBB68B17 CRC64;
MANGNEGDKT LLSICYNRGS LQLLDQRKLP LETIYLDIQD TKDGWDAIKE MVVRGAPAIA
IAAALSLAVE VSNLSSFDGT SDDASDFLSN KLDYLVSSRP TAVNLSDAAV KLKEVIKKAA
TTAKDANSVF QAYIEAAEVM LEDDVTSNKA IGSYGASFLK EHLTDSQKIT ILTHCNTGSL
ATAGYGTALG VIRALYTDGV LERAFCTETR PFNQGSRLTA FELVHENIPA TLIADSAAAA
LMNAGRVKAV IVGADRVAAN GDTANKIGTY NLAVSAMHHG IPFYVAAPLT SIDLSLSSGQ
EIVIEERSPK ELLHTRGGLG EQVAASGISV WNPAFDVTPA NLISGIITEK GVITKKGAET
FDIAEFVRKT SGNL
