7DLGT_CATRO
ID 7DLGT_CATRO Reviewed; 482 AA.
AC U5NH37;
DT 10-APR-2019, integrated into UniProtKB/Swiss-Prot.
DT 22-JAN-2014, sequence version 1.
DT 03-AUG-2022, entry version 31.
DE RecName: Full=7-deoxyloganetic acid glucosyl transferase {ECO:0000303|PubMed:24104568, ECO:0000303|PubMed:24710322};
DE Short=7-DLGT {ECO:0000303|PubMed:24104568, ECO:0000303|PubMed:24710322};
DE Short=Cr7DLGT {ECO:0000303|PubMed:24104568, ECO:0000303|PubMed:24710322};
DE EC=2.4.1.323 {ECO:0000269|PubMed:24104568, ECO:0000269|PubMed:24710322};
DE AltName: Full=UDP glycosyltransferase 709C2 {ECO:0000303|PubMed:24710322};
DE AltName: Full=UDP-sugar glycosyltransferase 8 {ECO:0000303|PubMed:24104568};
DE Short=Cr-UGT8 {ECO:0000303|PubMed:24104568};
GN Name=7DLGT {ECO:0000303|PubMed:24104568, ECO:0000303|PubMed:24710322};
GN Synonyms=UGT709C2 {ECO:0000303|PubMed:24710322},
GN UGT8 {ECO:0000303|PubMed:24104568};
GN ORFNames=Caros009839 {ECO:0000303|PubMed:24710322};
OS Catharanthus roseus (Madagascar periwinkle) (Vinca rosea).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Gentianales; Apocynaceae; Rauvolfioideae; Vinceae;
OC Catharanthinae; Catharanthus.
OX NCBI_TaxID=4058;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, CATALYTIC
RP ACTIVITY, PATHWAY, TISSUE SPECIFICITY, BIOPHYSICOCHEMICAL PROPERTIES, AND
RP DEVELOPMENTAL STAGE.
RC STRAIN=cv. Little Delicata;
RX PubMed=24104568; DOI=10.1105/tpc.113.115154;
RA Asada K., Salim V., Masada-Atsumi S., Edmunds E., Nagatoshi M.,
RA Terasaka K., Mizukami H., De Luca V.;
RT "A 7-deoxyloganetic Acid glucosyltransferase contributes a key step in
RT secologanin biosynthesis in madagascar periwinkle.";
RL Plant Cell 25:4123-4134(2013).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR
RP LOCATION, INDUCTION BY JASMONIC ACID, PATHWAY, AND BIOPHYSICOCHEMICAL
RP PROPERTIES.
RC STRAIN=cv. Little Bright Eyes;
RX PubMed=24710322; DOI=10.1038/ncomms4606;
RA Miettinen K., Dong L., Navrot N., Schneider T., Burlat V., Pollier J.,
RA Woittiez L., van der Krol S., Lugan R., Ilc T., Verpoorte R.,
RA Oksman-Caldentey K.M., Martinoia E., Bouwmeester H., Goossens A.,
RA Memelink J., Werck-Reichhart D.;
RT "The seco-iridoid pathway from Catharanthus roseus.";
RL Nat. Commun. 5:3606-3606(2014).
CC -!- FUNCTION: Component of the seco-iridoid and derivatives monoterpenoid
CC indole alkaloids (MIAs, e.g. vincristine, quinine, and strychnine)
CC biosynthesis pathway (PubMed:24710322). Catalyzes the glucosylation of
CC 7-deoxyloganetic acid to form 7-deoxyloganic acid using UDP-glucose as
CC the sugar donor (PubMed:24710322, PubMed:24104568). Inactive with
CC loganetic acid, loganetin, iridodial, iridotrial, 8-OH-geraniol,
CC jasmonic acid, gibberellic acid, indole acetic acid, salicylic acid,
CC abscisic acid, zeatin and luteolin (PubMed:24710322).
CC {ECO:0000269|PubMed:24104568, ECO:0000269|PubMed:24710322}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=7-deoxyloganetate + UDP-alpha-D-glucose = 7-deoxyloganate +
CC H(+) + UDP; Xref=Rhea:RHEA:39895, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:58223, ChEBI:CHEBI:58885, ChEBI:CHEBI:76844,
CC ChEBI:CHEBI:76846; EC=2.4.1.323;
CC Evidence={ECO:0000269|PubMed:24104568, ECO:0000269|PubMed:24710322};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=0.088 mM for 7-deoxyloganetic acid {ECO:0000269|PubMed:24104568};
CC KM=5.38 mM for UDP-glucose {ECO:0000269|PubMed:24104568};
CC Note=kcat is 0.130 sec(-1) with 7-deoxyloganetic acid as substrate.
CC kcat is 0.325 sec(-1) with UDP-glucose as substrate
CC (PubMed:24104568). kcat is 1.25 sec(-1) with 7-deoxyloganetic acid as
CC substrate (PubMed:24710322). {ECO:0000269|PubMed:24104568,
CC ECO:0000269|PubMed:24710322};
CC -!- PATHWAY: Alkaloid biosynthesis. {ECO:0000269|PubMed:24104568,
CC ECO:0000269|PubMed:24710322}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:24710322}. Cytoplasm,
CC cytosol {ECO:0000269|PubMed:24710322}.
CC -!- TISSUE SPECIFICITY: Expressed in the leaf internal phloem-associated
CC parenchyma (IPAP) inside the mesophyll. Mostly observed in leaves,
CC roots and stems, and, to a lower extent, in flowers.
CC {ECO:0000269|PubMed:24104568}.
CC -!- DEVELOPMENTAL STAGE: Highly expressed in the youngest leaves, and
CC levels gradually decrease in the older leaves.
CC {ECO:0000269|PubMed:24104568}.
CC -!- INDUCTION: By jasmonic acid (MeJA). {ECO:0000269|PubMed:24710322}.
CC -!- DISRUPTION PHENOTYPE: Large decline in secologanin and monoterpene
CC indole alkaloids (MIAs) accumulation. {ECO:0000269|PubMed:24104568}.
CC -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC {ECO:0000305}.
CC -!- WEB RESOURCE: Name=ORCAE database;
CC URL="https://bioinformatics.psb.ugent.be/orcae/overview/Catro";
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DR EMBL; KF415118; AGX93065.1; -; mRNA.
DR EMBL; KF302068; AHK60835.1; -; mRNA.
DR AlphaFoldDB; U5NH37; -.
DR SMR; U5NH37; -.
DR GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0102970; F:7-deoxyloganetic acid glucosyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0008194; F:UDP-glycosyltransferase activity; IEA:InterPro.
DR CDD; cd03784; GT1_Gtf-like; 1.
DR InterPro; IPR002213; UDP_glucos_trans.
DR InterPro; IPR035595; UDP_glycos_trans_CS.
DR Pfam; PF00201; UDPGT; 1.
DR PROSITE; PS00375; UDPGT; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Glycosyltransferase; Nucleus; Transferase.
FT CHAIN 1..482
FT /note="7-deoxyloganetic acid glucosyl transferase"
FT /id="PRO_0000446418"
FT BINDING 303
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250|UniProtKB:Q9M156"
FT BINDING 361..362
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250|UniProtKB:Q9M156"
FT BINDING 379..387
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250|UniProtKB:Q9M156"
FT BINDING 401..404
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250|UniProtKB:Q9M156"
SQ SEQUENCE 482 AA; 54271 MW; 12FB0F5397A25164 CRC64;
MGSQETNLPP HVLIFPLPIQ GHVNSMLRLA ELLCLAELDI TFIVSEFSHS RLIKHTNVAS
RFARYPGFQF QPISDGLPDD HPRAGERVMD ILPSTKNVTG PLFKQMMVEN KCFSSATRRP
ITCIIADGVL SFAGDFAQEK GIPLIYFRTV SACSFWACFC MPELIESGDI PIKGNGMDLI
VKSVPGMETF LRRRDLPGFC RVNDINEPKL QILKTETRQT TRAQAAILNT FEDLEGPILS
QIRKHMPRLF TIGPSHSHLT SRLETKNIKT LISSGSFWEE DRSCVDWLDA QPPRSVLYVS
FGSITVVTRD QLLEFWYGLV NSGQRFLWVM RPDSIMGKDG QSQIPADLEE GTKARGYMVG
WAPQEEVLNH PAIGGFLTHS GWNSTLESIV AGVPMICWPY FADQMINSRF VSEIWKIGLD
MKDTCDRETI VKMVRELMEI RKDEFLQRAD HMAKLAKEAV SEGGSSYSNL DGLVDYIKSL
II
