ID   MTO1_SCHPO              Reviewed;         666 AA.
AC   O13670;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 140.
DE   RecName: Full=Protein MTO1 homolog, mitochondrial;
DE   Flags: Precursor;
GN   Name=mto1; ORFNames=pi079, SPBC30B4.06c;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=10620777;
RX   DOI=10.1002/(sici)1097-0061(20000115)16:1<71::aid-yea505>3.0.co;2-5;
RA   Machida M., Yamazaki S., Kunihiro S., Tanaka T., Kushida N., Jinno K.,
RA   Haikawa Y., Yamazaki J., Yamamoto S., Sekine M., Oguchi A., Nagai Y.,
RA   Sakai M., Aoki K., Ogura K., Kudoh Y., Kikuchi H., Zhang M.Q., Yanagida M.;
RT   "A 38 kb segment containing the cdc2 gene from the left arm of fission
RT   yeast chromosome II: sequence analysis and characterization of the genomic
RT   DNA and cDNAs encoded on the segment.";
RL   Yeast 16:71-80(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
CC   -!- FUNCTION: Involved in the 5-carboxymethylaminomethyl modification
CC       (mnm(5)s(2)U34) of the wobble uridine base in mitochondrial tRNAs.
CC       {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000250};
CC   -!- INTERACTION:
CC       O13670; Q9USP7: SPBC902.06; NbExp=11; IntAct=EBI-1562568, EBI-1562556;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the MnmG family. {ECO:0000305}.
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DR   EMBL; AB004539; BAA21461.1; -; Genomic_DNA.
DR   EMBL; CU329671; CAA20319.1; -; Genomic_DNA.
DR   PIR; T40172; T40172.
DR   RefSeq; NP_595531.1; NM_001021441.2.
DR   AlphaFoldDB; O13670; -.
DR   SMR; O13670; -.
DR   BioGRID; 276943; 45.
DR   IntAct; O13670; 4.
DR   STRING; 4896.SPBC30B4.06c.1; -.
DR   MaxQB; O13670; -.
DR   PaxDb; O13670; -.
DR   EnsemblFungi; SPBC30B4.06c.1; SPBC30B4.06c.1:pep; SPBC30B4.06c.
DR   GeneID; 2540415; -.
DR   KEGG; spo:SPBC30B4.06c; -.
DR   PomBase; SPBC30B4.06c; -.
DR   VEuPathDB; FungiDB:SPBC30B4.06c; -.
DR   eggNOG; KOG2311; Eukaryota.
DR   HOGENOM; CLU_007831_2_2_1; -.
DR   InParanoid; O13670; -.
DR   OMA; FRPGYAI; -.
DR   PhylomeDB; O13670; -.
DR   PRO; PR:O13670; -.
DR   Proteomes; UP000002485; Chromosome II.
DR   GO; GO:0005759; C:mitochondrial matrix; IC:PomBase.
DR   GO; GO:0005739; C:mitochondrion; HDA:PomBase.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IBA:GO_Central.
DR   GO; GO:0032543; P:mitochondrial translation; ISO:PomBase.
DR   GO; GO:0070899; P:mitochondrial tRNA wobble uridine modification; IBA:GO_Central.
DR   GO; GO:0030488; P:tRNA methylation; IBA:GO_Central.
DR   GO; GO:0002098; P:tRNA wobble uridine modification; IBA:GO_Central.
DR   Gene3D; 1.10.150.570; -; 1.
DR   Gene3D; 3.50.50.60; -; 2.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR004416; MnmG.
DR   InterPro; IPR002218; MnmG-rel.
DR   InterPro; IPR020595; MnmG-rel_CS.
DR   InterPro; IPR026904; MnmG_C.
DR   InterPro; IPR044920; MnmG_C_subdom.
DR   InterPro; IPR040131; MnmG_N.
DR   PANTHER; PTHR11806; PTHR11806; 1.
DR   PANTHER; PTHR11806:SF0; PTHR11806:SF0; 1.
DR   Pfam; PF01134; GIDA; 1.
DR   Pfam; PF13932; GIDA_C; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
DR   TIGRFAMs; TIGR00136; gidA; 1.
DR   PROSITE; PS01280; GIDA_1; 1.
DR   PROSITE; PS01281; GIDA_2; 1.
PE   1: Evidence at protein level;
KW   FAD; Flavoprotein; Mitochondrion; Reference proteome; Transit peptide;
KW   tRNA processing.
FT   TRANSIT         1..16
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           ?..666
FT                   /note="Protein MTO1 homolog, mitochondrial"
FT                   /id="PRO_0000042790"
FT   BINDING         23..28
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   666 AA;  74230 MW;  703D4529BDEEA8B3 CRC64;
     MFRTTKRYFC TSFNRRKNVV VIGGGHAGVE AAAAASRLGA KTTLLTKSFD NIGQMSCNPA
     FGGIGKGTLM REIDALGGVV SGVCDESAIQ FHMLNRSNGP AVWSPRAQMD RSVFKKNMQK
     TISTYRKNLQ VREGAAVSIN VLTEDDGKQV CDSIVLEDGT AIPASCIVIT TGTFLGGQIN
     VGLTQLAAGR YGERPSLPLS KCLSNLGFKM GRLKTGTPPR LSSPINISKM TEQTGDEIPE
     TFSFLNLERD FSPALPQRSC YRTYTTELTH EIVRKNLAFA PHMLAGDILS PRYCPSLEAK
     VTRFPHKARH LIWLEPEGLD PNSWWYPNGL SNSMPEEIQH NIIRSIPGLE NCNIVRPAYG
     VMYDYVIPTQ LKATLETKKI QGLYLAGQIN GTTGYEEAAA QGILAGLNAG LSALGREPVD
     IPRNTALLGV MVDDLITKGV KEPYRVFTSR SEYRLTTRAD NADLRLTPLA QSIGLLDDQT
     HWESFQRTKS LLDFSNKIAK EFILSPQQWS KLGMPIPNDG KYRSAWDLLS FTNLDLFCVI
     SCIPKLKDIP KRVLQRLIIE GKYTYYIKRQ GTQNKQLNCR DESTVIPSDF DFDTLHSVSA
     EELMLLKTIR PATIGQLKRI QGIKPGTIIR LLRHTYYNPA KEAWLRKVYM PMYDMHSSEE
     NQSTNL